##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BbeI BcgI BciVI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BstAPI BstEII BstPI BstUI
CelII Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PinAI
PshAI PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 628 GTATAC GT^[AC][GT]AC
AclI 4244 AACGTT AA^CGTT
AflII 4461 CTTAAG C^TTAAG
AflIII 2492 ACACGT A^C[GA][CT]GT
AocI 3596 CCTAAGG CC^T[ACGT]AGG
AvaI 2392 CTCGAG C^[CT]CG[GA]G
BbrPI 2493 CACGTG CAC^GTG
BbvCI 62 CCTCAGC CCTCAGC(-5/-2)
BfrI 4461 CTTAAG C^TTAAG
BsaAI 2493 CACGTG [CT]AC^GT[GA]
BsaBI 1174 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 34 CGTCTC CGTCTC(1/5)
BsmI 553 GCATTC GAATGC(1/-1)
BsoBI 2392 CTCGAG C^[CT]CG[GA]G
BspMI 3825 ACCTGC ACCTGC(4/8)
BssSI 1771 CTCGTG CACGAG(-5/-1)
Bst1107I 628 GTATAC GTA^TAC
BstBI 292 TTCGAA TT^CGAA
BstZ17I 628 GTATAC GTA^TAC
Bsu36I 3596 CCTAAGG CC^T[ACGT]AGG
DrdI 1117 GACAAGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3596 CCTAAGG CC^T[ACGT]AGG
EcoNI 1912 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 2501 GGCGCT [GA]GCGC^[CT]
HgaI 1720 GCGTC GACGC(5/10)
HincII 904 GTTGAC GT[CT]^[GA]AC
HindII 904 GTTGAC GT[CT]^[GA]AC
MamI 1174 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3270 CAATTG C^AATTG
MunI 3270 CAATTG C^AATTG
NcoI 536 CCATGG C^CATGG
NdeI 4312 CATATG CA^TATG
NspV 292 TTCGAA TT^CGAA
PaeR7I 2392 CTCGAG C^TCGAG
PmaCI 2493 CACGTG CAC^GTG
PmeI 753 GTTTAAAC GTTT^AAAC
PmlI 2493 CACGTG CAC^GTG
Psp1406I 4244 AACGTT AA^CGTT
SapI 2284 GAAGAGC GCTCTTC(1/4)
ScaI 2327 AGTACT AGT^ACT
SexAI 157 ACCTGGT A^CC[AT]GGT
SfuI 292 TTCGAA TT^CGAA
SphI 3956 GCATGC GCATG^C
XbaI 1435 TCTAGA T^CTAGA
XhoI 2392 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AseI 3356 ATTAAT AT^TAAT
3473 ATTAAT
AsnI 3356 ATTAAT AT^TAAT
3473 ATTAAT
AspI 156 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4060 GACTCTGTC
BanI 1738 GGCACC G^G[CT][GA]CC
2387 GGTGCC
BclI 1052 TGATCA T^GATCA
2649 TGATCA
BcnI 1625 CCGGG CC^[GC]GG
2060 CCCGG
BglI 1684 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1730 GCCTTGCAGGC
CfoI 1676 GCGC GCG^C
2502 GCGC
DraIII 2382 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2653 CACATTGTG
EarI 3024 CTCTTC CTCTTC(1/4)
2284 GAAGAG
EcoT22I 2630 ATGCAT ATGCA^T
3673 ATGCAT
FauI 478 CCCGC CCCGC(4/6)
251 GCGGG
FbaI 1052 TGATCA T^GATCA
2649 TGATCA
HapII 1625 CCGG C^CGG
2061 CCGG
HhaI 1676 GCGC GCG^C
2502 GCGC
HinP1I 1676 GCGC G^CGC
2502 GCGC
HpaII 1625 CCGG C^CGG
2061 CCGG
Ksp632I 3024 CTCTTC CTCTTC(1/4)
2284 GAAGAG
MspI 1625 CCGG C^CGG
2061 CCGG
NciI 1625 CCGGG CC^[GC]GG
2060 CCCGG
NsiI 2630 ATGCAT ATGCA^T
3673 ATGCAT
PflFI 156 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4060 GACTCTGTC
PshBI 3356 ATTAAT AT^TAAT
3473 ATTAAT
Tth111I 156 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4060 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BamHI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI