##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AcsI AgeI
ApoI AscI AseI AsnI Asp700I
Asp718I AvrII BbeI BciVI BglII
BlnI BsiWI BsmBI BsmI BspDI
BspHI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoRI EcoRV FseI
HindIII KasI KpnI MfeI MluI
MunI NarI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3020 GTCGAC GT^[AC][GT]AC
AcyI 638 GGCGTC G[GA]^CG[CT]C
AflII 4554 CTTAAG C^TTAAG
AhdI 4603 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4603 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1601 TGCGCA TGC^GCA
BbrPI 2914 CACGTG CAC^GTG
BcgI 79 CGAGCGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2202 TGATCA T^GATCA
BfrI 4554 CTTAAG C^TTAAG
BsaAI 2914 CACGTG [CT]AC^GT[GA]
BsaBI 3609 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 638 GGCGTC G[GA]^CG[CT]C
BsiEI 1178 CGGTCG CG[GA][CT]^CG
Bsp1407I 4523 TGTACA T^GTACA
BspLU11I 336 ACATGT A^CATGT
BsrGI 4523 TGTACA T^GTACA
DraIII 3286 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4603 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2202 TGATCA T^GATCA
FspI 1601 TGCGCA TGC^GCA
Hin1I 638 GGCGTC G[GA]^CG[CT]C
KspI 1848 CCGCGG CCGC^GG
MamI 3609 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 497 GCCGGC GCC^GGC
NgoMIV 497 GCCGGC G^CCGGC
NheI 4095 GCTAGC G^CTAGC
PmaCI 2914 CACGTG CAC^GTG
PmlI 2914 CACGTG CAC^GTG
SacI 155 GAGCTC GAGCT^C
SacII 1848 CCGCGG CCGC^GG
SalI 3020 GTCGAC G^TCGAC
SmlI 4554 CTTAAG C^T[CT][GA]AG
SspBI 4523 TGTACA T^GTACA
SspI 5035 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 642 TCCGGA T^CCGGA
2496 TCCGGA
AflIII 336 ACATGT A^C[GA][CT]GT
2913 ACACGT
Alw44I 185 GTGCAC G^TGCAC
962 GTGCAC
ApaLI 185 GTGCAC G^TGCAC
962 GTGCAC
AspI 1206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3014 GACTGCGTC
BamHI 2195 GGATCC G^GATCC
2493 GGATCC
BbsI 710 GAAGAC GAAGAC(2/6)
4708 GAAGAC
BpuAI 710 GAAGAC GAAGAC(2/6)
4708 GAAGAC
BsaWI 642 TCCGGA [AT]^CCGG[AT]
2496 TCCGGA
BseAI 642 TCCGGA T^CCGGA
2496 TCCGGA
BspEI 642 TCCGGA T^CCGGA
2496 TCCGGA
DraI 4089 TTTAAA TTT^AAA
4119 TTTAAA
EcoT22I 3463 ATGCAT ATGCA^T
3771 ATGCAT
HpaI 4156 GTTAAC GTT^AAC
4303 GTTAAC
MroI 642 TCCGGA T^CCGGA
2496 TCCGGA
NsiI 3463 ATGCAT ATGCA^T
3771 ATGCAT
PflFI 1206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3014 GACTGCGTC
SapI 3386 GAAGAGC GCTCTTC(1/4)
5041 GAAGAGC
SphI 951 GCATGC GCATG^C
4182 GCATGC
Tth111I 1206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3014 GACTGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmaI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI