##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AccIII AflII AgeI AscI
AseI AsnI AviII BcgI BfrI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoO65I EcoRV FseI FspI HindIII
HpaI KspI MfeI MluI MroI
MunI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SfiI SfuI SgrAI SnaBI SpeI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1484 GACGTC GACGT^C
Acc65I 1074 GGTACC G^GTACC
AclI 4308 AACGTT AA^CGTT
AhdI 1448 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1101 AGCGCT AGC^GCT
Asp718I 1074 GGTACC G^GTACC
AspEI 1448 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1555 CCTAGG C^CTAGG
BbrPI 4282 CACGTG CAC^GTG
BbvCI 1156 GCTGAGG CCTCAGC(-5/-2)
BglII 2457 AGATCT A^GATCT
BlnI 1555 CCTAGG C^CTAGG
BlpI 1306 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1306 GCTCAGC GC^T[ACGT]AGC
BsiEI 1820 CGACCG CG[GA][CT]^CG
CelII 1306 GCTCAGC GC^T[ACGT]AGC
DraI 4181 TTTAAA TTT^AAA
DraIII 3226 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1448 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1101 AGCGCT AGC^GCT
EcoRI 2206 GAATTC G^AATTC
EcoT22I 4365 ATGCAT ATGCA^T
KpnI 1074 GGTACC GGTAC^C
NaeI 3306 GCCGGC GCC^GGC
NdeI 4387 CATATG CA^TATG
NgoMIV 3306 GCCGGC G^CCGGC
NsiI 4365 ATGCAT ATGCA^T
PmaCI 4282 CACGTG CAC^GTG
PmlI 4282 CACGTG CAC^GTG
Psp1406I 4308 AACGTT AA^CGTT
SacI 1295 GAGCTC GAGCT^C
SbfI 3010 CCTGCAGG CCTGCA^GG
SexAI 2214 ACCTGGT A^CC[AT]GGT
SmaI 3379 CCCGGG CCC^GGG
SphI 1218 GCATGC GCATG^C
Sse8387I 3010 CCTGCAGG CCTGCA^GG
SspI 98 AATATT AAT^ATT
XmaCI 3379 CCCGGG C^CCGGG
XmaI 3379 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 3205 GTGCAC G^TGCAC
4016 GTGCAC
ApaI 807 GGGCCC GGGCC^C
1068 GGGCCC
ApaLI 3205 GTGCAC G^TGCAC
4016 GTGCAC
Asp700I 1186 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3963 GAATGAGTTC
AspI 695 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
866 GACTGTGTC
BalI 120 TGGCCA TGG^CCA
216 TGGCCA
BamHI 3654 GGATCC G^GATCC
3926 GGATCC
BciVI 2155 GGATAC GTATCC(6/5)
4331 GGATAC
BglI 1038 GCCAGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3179 GCCGTGGTGGC
BsaAI 776 TACGTG [CT]AC^GT[GA]
4282 CACGTG
BsaBI 394 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2773 GATCCTGATC
Bsp1407I 2748 TGTACA T^GTACA
4286 TGTACA
BspHI 960 TCATGA T^CATGA
1785 TCATGA
BspLU11I 2646 ACATGT A^CATGT
4398 ACATGT
BsrDI 3124 CATTGC GCAATG(2/0)
2415 GCAATG
BsrFI 1141 ACCGGC [GA]^CCGG[CT]
3306 GCCGGC
BsrGI 2748 TGTACA T^GTACA
4286 TGTACA
BstXI 2376 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2565 CCAACTCCTTGG
Cfr10I 1141 ACCGGC [GA]^CCGG[CT]
3306 GCCGGC
EarI 983 CTCTTC CTCTTC(1/4)
194 GAAGAG
HincII 2387 GTCAAC GT[CT]^[GA]AC
4499 GTTGAC
HindII 2387 GTCAAC GT[CT]^[GA]AC
4499 GTTGAC
Ksp632I 983 CTCTTC CTCTTC(1/4)
194 GAAGAG
MamI 394 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2773 GATCCTGATC
MluNI 120 TGGCCA TGG^CCA
216 TGGCCA
MscI 120 TGGCCA TGG^CCA
216 TGGCCA
PflFI 695 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
866 GACTGTGTC
RcaI 960 TCATGA T^CATGA
1785 TCATGA
SapI 194 GAAGAGC GCTCTTC(1/4)
982 GCTCTTC
ScaI 201 AGTACT AGT^ACT
849 AGTACT
SspBI 2748 TGTACA T^GTACA
4286 TGTACA
Tth111I 695 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
866 GACTGTGTC
XmnI 1186 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3963 GAATGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII