##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AflII AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BamHI BciVI
BclI BfrI BglII BsiWI BspDI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI Eam1105I EarI Eco47III EcoO65I
EcoRV FbaI FseI FspI KpnI
Ksp632I MluI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI SfuI SnaBI
SpeI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2634 GACGTC GACGT^C
AccIII 3949 TCCGGA T^CCGGA
AgeI 768 ACCGGT A^CCGGT
Asp700I 325 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2992 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1010 CCCGGG C^[CT]CG[GA]G
BalI 2190 TGGCCA TGG^CCA
BbeI 1535 GGCGCC GGCGC^C
BlpI 782 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 782 GCTGAGC GC^T[ACGT]AGC
BsaBI 2710 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1372 GGTCTC GGTCTC(1/5)
BseAI 3949 TCCGGA T^CCGGA
BsmBI 1414 CGTCTC CGTCTC(1/5)
BsmI 991 GAATGC GAATGC(1/-1)
BsoBI 1010 CCCGGG C^[CT]CG[GA]G
Bsp1407I 4388 TGTACA T^GTACA
BspEI 3949 TCCGGA T^CCGGA
BspHI 3103 TCATGA T^CATGA
BsrGI 4388 TGTACA T^GTACA
CelII 782 GCTGAGC GC^T[ACGT]AGC
EcoRI 2837 GAATTC G^AATTC
EcoT22I 3096 ATGCAT ATGCA^T
HpaI 4435 GTTAAC GTT^AAC
KasI 1535 GGCGCC G^GCGCC
MamI 2710 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4090 CAATTG C^AATTG
MluNI 2190 TGGCCA TGG^CCA
MroI 3949 TCCGGA T^CCGGA
MscI 2190 TGGCCA TGG^CCA
MunI 4090 CAATTG C^AATTG
NarI 1535 GGCGCC GG^CGCC
NsiI 3096 ATGCAT ATGCA^T
PflFI 2992 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 214 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 768 ACCGGT A^CCGGT
PshAI 1460 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3103 TCATGA T^CATGA
ScaI 357 AGTACT AGT^ACT
SfiI 2191 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1535 GGCGCC GGC^GCC
SgrAI 767 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1010 CCCGGG CCC^GGG
SphI 714 GCATGC GCATG^C
SspBI 4388 TGTACA T^GTACA
SwaI 4491 ATTTAAAT ATTT^AAAT
Tth111I 2992 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 214 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2386 TCTAGA T^CTAGA
XcmI 1731 CCAAAGGGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1010 CCCGGG C^CCGGG
XmaI 1010 CCCGGG C^CCGGG
XmnI 325 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2637 GTCTAC GT^[AC][GT]AC
3377 GTAGAC
AflIII 2561 ACACGT A^C[GA][CT]GT
3409 ACACGT
AocI 1067 CCTCAGG CC^T[ACGT]AGG
3000 CCTGAGG
ApaI 3492 GGGCCC GGGCC^C
3800 GGGCCC
AvrII 2269 CCTAGG C^CTAGG
3261 CCTAGG
BbrPI 487 CACGTG CAC^GTG
2562 CACGTG
BcgI 3628 CGAAGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
741 GCATCACCATCG
BlnI 2269 CCTAGG C^CTAGG
3261 CCTAGG
BsaWI 768 ACCGGT [AT]^CCGG[AT]
3949 TCCGGA
BsiEI 348 CGGCCG CG[GA][CT]^CG
1944 CGGCCG
BstAPI 1433 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1915 GCAGAGGCTGC
BstYI 756 AGATCC [GA]^GATC[CT]
2584 GGATCT
Bsu36I 1067 CCTCAGG CC^T[ACGT]AGG
3000 CCTGAGG
DrdI 907 GACGGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2089 GACCGGCGCGTC
EagI 348 CGGCCG C^GGCCG
1944 CGGCCG
EclXI 348 CGGCCG C^GGCCG
1944 CGGCCG
Eco52I 348 CGGCCG C^GGCCG
1944 CGGCCG
Eco81I 1067 CCTCAGG CC^T[ACGT]AGG
3000 CCTGAGG
HaeII 865 GGCGCT [GA]GCGC^[CT]
1535 GGCGCC
HincII 2730 GTCAAC GT[CT]^[GA]AC
4435 GTTAAC
HindII 2730 GTCAAC GT[CT]^[GA]AC
4435 GTTAAC
KspI 1028 CCGCGG CCGC^GG
1494 CCGCGG
MflI 756 AGATCC [GA]^GATC[CT]
2584 GGATCT
NaeI 1868 GCCGGC GCC^GGC
1978 GCCGGC
NgoMIV 1868 GCCGGC G^CCGGC
1978 GCCGGC
NspI 234 ACATGC [GA]CATG^[CT]
714 GCATGC
PmaCI 487 CACGTG CAC^GTG
2562 CACGTG
PmlI 487 CACGTG CAC^GTG
2562 CACGTG
SacII 1028 CCGCGG CCGC^GG
1494 CCGCGG
SexAI 213 ACCAGGT A^CC[AT]GGT
3961 ACCAGGT
XhoII 756 AGATCC [GA]^GATC[CT]
2584 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I