##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI Aor51HI AscI AviII AvrII
BbeI BbrPI BcgI BclI BfrI
BlnI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrFI BsrGI BssHII BstBI BstUI
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRI FbaI FseI
FspI HindIII KasI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 4584 GGTACC G^GTACC
AcyI 8 GACGCC G[GA]^CG[CT]C
AhdI 8 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2522 GTGCAC G^TGCAC
ApaI 1434 GGGCCC GGGCC^C
ApaLI 2522 GTGCAC G^TGCAC
AseI 3105 ATTAAT AT^TAAT
AsnI 3105 ATTAAT AT^TAAT
Asp718I 4584 GGTACC G^GTACC
AspEI 8 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2818 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1550 GGATCC G^GATCC
BsaAI 1528 TACGTG [CT]AC^GT[GA]
BsaBI 2442 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 8 GACGCC G[GA]^CG[CT]C
BsrBI 982 GAGCGG CCGCTC(-3/-3)
Bst1107I 3929 GTATAC GTA^TAC
BstEII 2896 GGTCACC G^GT[ACGT]ACC
BstPI 2896 GGTCACC G^GT[ACGT]ACC
BstZ17I 3929 GTATAC GTA^TAC
DraI 3639 TTTAAA TTT^AAA
DraIII 3548 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 8 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2896 GGTCACC G^GT[ACGT]ACC
EcoRV 2263 GATATC GAT^ATC
HaeII 423 GGCGCT [GA]GCGC^[CT]
HgaI 8 GACGC GACGC(5/10)
Hin1I 8 GACGCC G[GA]^CG[CT]C
KpnI 4584 GGTACC GGTAC^C
MamI 2442 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 938 CTCGAG C^TCGAG
PflFI 2818 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3105 ATTAAT AT^TAAT
PstI 214 CTGCAG CTGCA^G
SexAI 3682 ACCAGGT A^CC[AT]GGT
SmlI 938 CTCGAG C^T[CT][GA]AG
SpeI 3479 ACTAGT A^CTAGT
SphI 3990 GCATGC GCATG^C
TaqI 939 TCGA T^CGA
Tth111I 2818 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 939 TCGA T^CGA
XhoI 938 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 1210 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2011 CAGTATCTG
AvaI 938 CTCGAG C^[CT]CG[GA]G
1225 CCCGAG
BbvCI 342 CCTCAGC CCTCAGC(-5/-2)
229 GCTGAGG
BcnI 609 CCGGG CC^[GC]GG
815 CCGGG
BlpI 205 GCTAAGC GC^T[ACGT]AGC
1282 GCTGAGC
Bpu1102I 205 GCTAAGC GC^T[ACGT]AGC
1282 GCTGAGC
BsoBI 938 CTCGAG C^[CT]CG[GA]G
1225 CCCGAG
BspHI 3342 TCATGA T^CATGA
3622 TCATGA
BspMI 724 ACCTGC ACCTGC(4/8)
3912 GCAGGT
BssSI 454 CACGAG CACGAG(-5/-1)
1570 CACGAG
BstAPI 123 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3903 GCAGAACGTGC
CelII 205 GCTAAGC GC^T[ACGT]AGC
1282 GCTGAGC
CfoI 45 GCGC GCG^C
424 GCGC
DrdI 163 GACTTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
978 GACTGAGCGGTC
EarI 3062 CTCTTC CTCTTC(1/4)
1125 GAAGAG
EcoT22I 1348 ATGCAT ATGCA^T
3992 ATGCAT
HapII 609 CCGG C^CGG
815 CCGG
HhaI 45 GCGC GCG^C
424 GCGC
HinP1I 45 GCGC G^CGC
424 GCGC
HpaI 2501 GTTAAC GTT^AAC
2829 GTTAAC
HpaII 609 CCGG C^CGG
815 CCGG
Ksp632I 3062 CTCTTC CTCTTC(1/4)
1125 GAAGAG
MspI 609 CCGG C^CGG
815 CCGG
NciI 609 CCGGG CC^[GC]GG
815 CCGGG
NsiI 1348 ATGCAT ATGCA^T
3992 ATGCAT
PflMI 1443 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2047 CCACTAGATGG
PpuMI 536 GGGTCCC [GA]G^G[AT]CC[CT]
1220 AGGACCC
RcaI 3342 TCATGA T^CATGA
3622 TCATGA
Van91I 1443 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2047 CCACTAGATGG
XbaI 3279 TCTAGA T^CTAGA
3973 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BciVI BfaI BglI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmnI