##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI ApaLI
AscI Asp718I AspEI AspHI AviII
AvrII BbeI BciVI BclI BglI
BlnI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRV FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
PvuI RsrII SacI SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AclI 1563 AACGTT AA^CGTT
Aor51HI 656 AGCGCT AGC^GCT
ApaI 1657 GGGCCC GGGCC^C
AvaI 1969 CCCGAG C^[CT]CG[GA]G
BamHI 4175 GGATCC G^GATCC
BbrPI 1013 CACGTG CAC^GTG
BbvCI 3004 GCTGAGG CCTCAGC(-5/-2)
BcgI 1952 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2704 AGATCT A^GATCT
BlpI 3782 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3782 GCTTAGC GC^T[ACGT]AGC
BsoBI 1969 CCCGAG C^[CT]CG[GA]G
CelII 3782 GCTTAGC GC^T[ACGT]AGC
DsaI 4084 CCACGG C^C[GA][CT]GG
Eco47III 656 AGCGCT AGC^GCT
EcoNI 912 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 656 AGCGCT [GA]GCGC^[CT]
HgaI 231 GACGC GACGC(5/10)
NdeI 4514 CATATG CA^TATG
PmaCI 1013 CACGTG CAC^GTG
PmlI 1013 CACGTG CAC^GTG
Psp1406I 1563 AACGTT AA^CGTT
SapI 4482 GAAGAGC GCTCTTC(1/4)
SbfI 251 CCTGCAGG CCTGCA^GG
SexAI 572 ACCTGGT A^CC[AT]GGT
Sse8387I 251 CCTGCAGG CCTGCA^GG
XbaI 3042 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AseI 2570 ATTAAT AT^TAAT
3662 ATTAAT
AsnI 2570 ATTAAT AT^TAAT
3662 ATTAAT
Asp700I 274 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
939 GAAAGAATTC
AspI 984 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1871 GACGGAGTC
AvaII 4019 GGTCC G^G[AT]CC
4746 GGACC
BalI 933 TGGCCA TGG^CCA
2051 TGGCCA
BanI 2986 GGTGCC G^G[CT][GA]CC
3912 GGTGCC
BanII 593 GAGCCC G[GA]GC[CT]^C
1657 GGGCCC
BbsI 3459 GAAGAC GAAGAC(2/6)
4187 GTCTTC
BcnI 697 CCCGG CC^[GC]GG
3955 CCCGG
BpuAI 3459 GAAGAC GAAGAC(2/6)
4187 GTCTTC
BsaAI 1013 CACGTG [CT]AC^GT[GA]
2264 TACGTG
BsaI 669 GGTCTC GGTCTC(1/5)
2061 GGTCTC
BsmI 2600 GCATTC GAATGC(1/-1)
2680 GCATTC
BspLU11I 155 ACATGT A^CATGT
3517 ACATGT
BspMI 1539 ACCTGC ACCTGC(4/8)
254 GCAGGT
BsrDI 3019 CATTGC GCAATG(2/0)
2086 GCAATG
BssSI 1889 CACGAG CACGAG(-5/-1)
2648 CTCGTG
BstAPI 786 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2713 GCACGACGTGC
BstXI 3234 CCAGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3705 CCATTGTTGTGG
DraII 1656 GGGGCCC [GA]G^G[ACGT]CC[CT]
4001 AGGCCCC
DraIII 695 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2714 CACGACGTG
EcoO109I 1656 GGGGCCC [GA]G^G[ACGT]CC[CT]
4001 AGGCCCC
EcoRI 943 GAATTC G^AATTC
1065 GAATTC
EcoT14I 302 CCAAGG C^C[AT][AT]GG
963 CCTTGG
EcoT22I 869 ATGCAT ATGCA^T
2566 ATGCAT
FauI 1751 CCCGC CCCGC(4/6)
3085 CCCGC
HapII 698 CCGG C^CGG
3956 CCGG
HpaII 698 CCGG C^CGG
3956 CCGG
MluNI 933 TGGCCA TGG^CCA
2051 TGGCCA
MscI 933 TGGCCA TGG^CCA
2051 TGGCCA
MspI 698 CCGG C^CGG
3956 CCGG
NciI 697 CCCGG CC^[GC]GG
3955 CCCGG
NsiI 869 ATGCAT ATGCA^T
2566 ATGCAT
PflFI 984 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1871 GACGGAGTC
PshBI 2570 ATTAAT AT^TAAT
3662 ATTAAT
StyI 302 CCAAGG C^C[AT][AT]GG
963 CCTTGG
Tth111I 984 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1871 GACGGAGTC
XmnI 274 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
939 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
BbvI BfaI BfrI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DrdI EaeI EarI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII