##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI Asp718I AvrII BbeI BbrPI
BbvCI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII BstBI CelII ClaI CpoI
EagI EclXI Eco52I EcoT22I FbaI
FseI KasI KpnI KspI MluI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 1103 CGCG CG^CG
AccIII 1544 TCCGGA T^CCGGA
AcyI 2138 GGCGTC G[GA]^CG[CT]C
AflIII 4223 ACACGT A^C[GA][CT]GT
AhdI 1329 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4325 GTGCAC G^TGCAC
Aor51HI 4934 AGCGCT AGC^GCT
ApaLI 4325 GTGCAC G^TGCAC
AspEI 1329 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3382 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 161 TGCGCA TGC^GCA
BanI 12 GGCACC G^G[CT][GA]CC
BsaHI 2138 GGCGTC G[GA]^CG[CT]C
BsaWI 1544 TCCGGA [AT]^CCGG[AT]
BseAI 1544 TCCGGA T^CCGGA
BsmI 1634 GAATGC GAATGC(1/-1)
BspEI 1544 TCCGGA T^CCGGA
BspHI 249 TCATGA T^CATGA
BsrDI 4653 CATTGC GCAATG(2/0)
Bst1107I 2853 GTATAC GTA^TAC
BstEII 2076 GGTGACC G^GT[ACGT]ACC
BstPI 2076 GGTGACC G^GT[ACGT]ACC
BstUI 1103 CGCG CG^CG
BstXI 1750 CCAACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2853 GTATAC GTA^TAC
DraIII 2971 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1329 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4934 AGCGCT AGC^GCT
EcoO65I 2076 GGTGACC G^GT[ACGT]ACC
EcoRV 1522 GATATC GAT^ATC
FspI 161 TGCGCA TGC^GCA
Hin1I 2138 GGCGTC G[GA]^CG[CT]C
HpaI 3675 GTTAAC GTT^AAC
MfeI 1889 CAATTG C^AATTG
MroI 1544 TCCGGA T^CCGGA
MunI 1889 CAATTG C^AATTG
MvnI 1103 CGCG CG^CG
NaeI 283 GCCGGC GCC^GGC
NgoMIV 283 GCCGGC G^CCGGC
NspI 128 ACATGC [GA]CATG^[CT]
PflFI 3382 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 249 TCATGA T^CATGA
SapI 3793 GAAGAGC GCTCTTC(1/4)
ScaI 2423 AGTACT AGT^ACT
SexAI 1133 ACCTGGT A^CC[AT]GGT
SmaI 515 CCCGGG CCC^GGG
SspI 1384 AATATT AAT^ATT
Tth111I 3382 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4054 TCTAGA T^CTAGA
XmaCI 515 CCCGGG C^CCGGG
XmaI 515 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AocI 1647 CCTGAGG CC^T[ACGT]AGG
4495 CCTGAGG
ApaI 4142 GGGCCC GGGCC^C
4647 GGGCCC
AseI 535 ATTAAT AT^TAAT
5026 ATTAAT
AsnI 535 ATTAAT AT^TAAT
5026 ATTAAT
AvaI 515 CCCGGG C^[CT]CG[GA]G
2914 CCCGAG
BamHI 1541 GGATCC G^GATCC
3830 GGATCC
BbsI 499 GTCTTC GAAGAC(2/6)
1358 GTCTTC
BciVI 4980 GGATAC GTATCC(6/5)
3022 GTATCC
BglI 1021 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2664 GCCTGTTCGGC
BpuAI 499 GTCTTC GAAGAC(2/6)
1358 GTCTTC
BsaBI 2302 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3721 GATGGACATC
BsgI 2842 CTGCAC GTGCAG(16/14)
3548 GTGCAG
BsoBI 515 CCCGGG C^[CT]CG[GA]G
2914 CCCGAG
BsrBI 3219 CCGCTC CCGCTC(-3/-3)
1171 GAGCGG
BsrFI 283 GCCGGC [GA]^CCGG[CT]
2210 ACCGGC
BstAPI 164 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1217 GCACTGACTGC
Bsu36I 1647 CCTGAGG CC^T[ACGT]AGG
4495 CCTGAGG
Cfr10I 283 GCCGGC [GA]^CCGG[CT]
2210 ACCGGC
DraI 1801 TTTAAA TTT^AAA
2958 TTTAAA
Eco81I 1647 CCTGAGG CC^T[ACGT]AGG
4495 CCTGAGG
EcoRI 220 GAATTC G^AATTC
1141 GAATTC
HaeII 2570 GGCGCT [GA]GCGC^[CT]
4934 AGCGCT
HincII 1942 GTTGAC GT[CT]^[GA]AC
3675 GTTAAC
HindII 1942 GTTGAC GT[CT]^[GA]AC
3675 GTTAAC
MamI 2302 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3721 GATGGACATC
NdeI 821 CATATG CA^TATG
1716 CATATG
PshBI 535 ATTAAT AT^TAAT
5026 ATTAAT
SbfI 261 CCTGCAGG CCTGCA^GG
597 CCTGCAGG
Sse8387I 261 CCTGCAGG CCTGCA^GG
597 CCTGCAGG
XcmI 701 CCAGCCTCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1750 CCAACAACTTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanII BbvI BcnI
BfaI BglII BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI