##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BcgI BclI BglII
BlnI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoRV
EcoT22I FbaI FseI FspI MamI
MluI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SfiI SfuI SnaBI
SpeI SphI SspBI SwaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1475 GTAGAC GT^[AC][GT]AC
AccIII 3675 TCCGGA T^CCGGA
AflII 489 CTTAAG C^TTAAG
AocI 2596 CCTCAGG CC^T[ACGT]AGG
Asp700I 4434 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1691 TGGCCA TGG^CCA
BciVI 86 GGATAC GTATCC(6/5)
BfrI 489 CTTAAG C^TTAAG
BsaWI 3675 TCCGGA [AT]^CCGG[AT]
BseAI 3675 TCCGGA T^CCGGA
BspEI 3675 TCCGGA T^CCGGA
BsrBI 3531 GAGCGG CCGCTC(-3/-3)
BssHII 3553 GCGCGC G^CGCGC
Bsu36I 2596 CCTCAGG CC^T[ACGT]AGG
EarI 4056 GAAGAG CTCTTC(1/4)
Eco81I 2596 CCTCAGG CC^T[ACGT]AGG
EcoRI 4377 GAATTC G^AATTC
HindIII 4683 AAGCTT A^AGCTT
HpaI 3599 GTTAAC GTT^AAC
Ksp632I 4056 GAAGAG CTCTTC(1/4)
MfeI 4297 CAATTG C^AATTG
MluNI 1691 TGGCCA TGG^CCA
MroI 3675 TCCGGA T^CCGGA
MscI 1691 TGGCCA TGG^CCA
MunI 4297 CAATTG C^AATTG
NdeI 1202 CATATG CA^TATG
NheI 3847 GCTAGC G^CTAGC
PaeR7I 3759 CTCGAG C^TCGAG
SexAI 10 ACCAGGT A^CC[AT]GGT
SspI 4697 AATATT AAT^ATT
XbaI 4442 TCTAGA T^CTAGA
XhoI 3759 CTCGAG C^TCGAG
XmnI 4434 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1410 GACGTC GACGT^C
1583 GACGTC
Acc65I 723 GGTACC G^GTACC
1246 GGTACC
Asp718I 723 GGTACC G^GTACC
1246 GGTACC
AspI 2112 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2574 GACTCTGTC
BlpI 420 GCTCAGC GC^T[ACGT]AGC
2406 GCTGAGC
Bpu1102I 420 GCTCAGC GC^T[ACGT]AGC
2406 GCTGAGC
CelII 420 GCTCAGC GC^T[ACGT]AGC
2406 GCTGAGC
EagI 1764 CGGCCG C^GGCCG
2247 CGGCCG
EclXI 1764 CGGCCG C^GGCCG
2247 CGGCCG
Eco52I 1764 CGGCCG C^GGCCG
2247 CGGCCG
KpnI 723 GGTACC GGTAC^C
1246 GGTACC
NspI 617 GCATGT [GA]CATG^[CT]
1099 GCATGT
PflFI 2112 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2574 GACTCTGTC
PflMI 1976 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3123 CCACTCTGTGG
SacI 532 GAGCTC GAGCT^C
3326 GAGCTC
SbfI 715 CCTGCAGG CCTGCA^GG
2469 CCTGCAGG
SgrAI 2499 CGCCGGCG C[GA]^CCGG[CT]G
2866 CGCCGGCG
SmaI 2169 CCCGGG CCC^GGG
2562 CCCGGG
SmlI 489 CTTAAG C^T[CT][GA]AG
3759 CTCGAG
Sse8387I 715 CCTGCAGG CCTGCA^GG
2469 CCTGCAGG
StuI 120 AGGCCT AGG^CCT
1793 AGGCCT
Tth111I 2112 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2574 GACTCTGTC
Van91I 1976 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3123 CCACTCTGTGG
XmaCI 2169 CCCGGG C^CCGGG
2562 CCCGGG
XmaI 2169 CCCGGG C^CCGGG
2562 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII