##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BcgI BfrI BglII
BlnI BsaBI BseAI BsiEI BspDI
BspEI BspLU11I Bst1107I BstBI BstYI
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FspI HincII HindII
HpaI KpnI MamI MflI MluI
MroI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XhoII
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 323 GTCTAC GT^[AC][GT]AC
AflIII 2340 ACGTGT A^C[GA][CT]GT
AocI 4425 CCTGAGG CC^T[ACGT]AGG
BbeI 986 GGCGCC GGCGC^C
BbrPI 790 CACGTG CAC^GTG
BbsI 2555 GTCTTC GAAGAC(2/6)
BbvCI 2161 CCTCAGC CCTCAGC(-5/-2)
BclI 646 TGATCA T^GATCA
BpuAI 2555 GTCTTC GAAGAC(2/6)
BsaAI 790 CACGTG [CT]AC^GT[GA]
BsiWI 3282 CGTACG C^GTACG
BsmI 4447 GAATGC GAATGC(1/-1)
Bsp1407I 346 TGTACA T^GTACA
BspHI 1035 TCATGA T^CATGA
BsrDI 454 CATTGC GCAATG(2/0)
BsrGI 346 TGTACA T^GTACA
BssHII 3751 GCGCGC G^CGCGC
BssSI 1437 CACGAG CACGAG(-5/-1)
BstEII 1084 GGTGACC G^GT[ACGT]ACC
BstPI 1084 GGTGACC G^GT[ACGT]ACC
BstXI 598 CCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4425 CCTGAGG CC^T[ACGT]AGG
DraI 4514 TTTAAA TTT^AAA
DraIII 464 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4425 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1084 GGTGACC G^GT[ACGT]ACC
FbaI 646 TGATCA T^GATCA
HindIII 2583 AAGCTT A^AGCTT
KasI 986 GGCGCC G^GCGCC
KspI 439 CCGCGG CCGC^GG
MfeI 180 CAATTG C^AATTG
MunI 180 CAATTG C^AATTG
NarI 986 GGCGCC GG^CGCC
NcoI 411 CCATGG C^CATGG
PaeR7I 1013 CTCGAG C^TCGAG
PmaCI 790 CACGTG CAC^GTG
PmlI 790 CACGTG CAC^GTG
RcaI 1035 TCATGA T^CATGA
SacII 439 CCGCGG CCGC^GG
ScaI 687 AGTACT AGT^ACT
SfoI 986 GGCGCC GGC^GCC
SphI 3114 GCATGC GCATG^C
SspBI 346 TGTACA T^GTACA
XhoI 1013 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 4538 AAATTC [GA]^AATT[CT]
4549 AAATTT
AcyI 986 GGCGCC G[GA]^CG[CT]C
4096 GGCGTC
ApoI 4538 AAATTC [GA]^AATT[CT]
4549 AAATTT
Asp700I 25 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2581 GAAAGCTTTC
BalI 1433 TGGCCA TGG^CCA
2599 TGGCCA
BciVI 4258 GGATAC GTATCC(6/5)
3876 GTATCC
BfaI 1092 CTAG C^TAG
2232 CTAG
BsaHI 986 GGCGCC G[GA]^CG[CT]C
4096 GGCGTC
BsaI 1901 GAGACC GGTCTC(1/5)
2750 GGTCTC
BsaWI 1201 TCCGGT [AT]^CCGG[AT]
2413 ACCGGA
EcoNI 1033 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4475 CCTTGTTTAGG
FseI 61 GGCCGGCC GGCCGG^CC
3409 GGCCGGCC
HaeII 986 GGCGCC [GA]GCGC^[CT]
3967 GGCGCT
HgaI 3131 GACGC GACGC(5/10)
4097 GCGTC
Hin1I 986 GGCGCC G[GA]^CG[CT]C
4096 GGCGTC
MaeI 1092 CTAG C^TAG
2232 CTAG
MluNI 1433 TGGCCA TGG^CCA
2599 TGGCCA
MscI 1433 TGGCCA TGG^CCA
2599 TGGCCA
NspI 1234 ACATGC [GA]CATG^[CT]
3114 GCATGC
SapI 3631 GAAGAGC GCTCTTC(1/4)
2902 GCTCTTC
SfiI 1434 GGCCACGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3640 GGCCCGGCTGGCC
SmlI 431 CTTGAG C^T[CT][GA]AG
1013 CTCGAG
StuI 119 AGGCCT AGG^CCT
213 AGGCCT
TfiI 620 GAATC G^A[AT]TC
2507 GAATC
XmnI 25 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2581 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI ApaI ApaLI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI