##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI BfrI
BglII BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrFI BssHII BstEII
BstPI Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FseI HpaI MfeI MluI
MroI MunI NaeI NgoMIV NotI
NruI PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfiI SgrAI SpeI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AhdI 3794 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 806 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3794 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2896 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2846 TGCGCA TGC^GCA
BbvCI 3143 CCTCAGC CCTCAGC(-5/-2)
BcgI 2303 GCATCTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2324 TGATCA T^GATCA
Bsp1407I 4526 TGTACA T^GTACA
BsrGI 4526 TGTACA T^GTACA
Bst1107I 2671 GTATAC GTA^TAC
BstAPI 409 GCACGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4042 TTCGAA TT^CGAA
BstZ17I 2671 GTATAC GTA^TAC
DraI 4036 TTTAAA TTT^AAA
DraIII 2873 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3794 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 794 ATGCAT ATGCA^T
FbaI 2324 TGATCA T^GATCA
FspI 2846 TGCGCA TGC^GCA
HincII 225 GTCAAC GT[CT]^[GA]AC
HindII 225 GTCAAC GT[CT]^[GA]AC
HindIII 2602 AAGCTT A^AGCTT
KspI 3599 CCGCGG CCGC^GG
NdeI 2739 CATATG CA^TATG
NheI 4623 GCTAGC G^CTAGC
NsiI 794 ATGCAT ATGCA^T
NspV 4042 TTCGAA TT^CGAA
PflFI 2896 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3719 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2094 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3599 CCGCGG CCGC^GG
SexAI 2813 ACCTGGT A^CC[AT]GGT
SfuI 4042 TTCGAA TT^CGAA
SnaBI 2277 TACGTA TAC^GTA
SspBI 4526 TGTACA T^GTACA
Tth111I 2896 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 806 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 3360 GGTACC G^GTACC
4701 GGTACC
AccI 2569 GTCTAC GT^[AC][GT]AC
2671 GTATAC
AccII 2502 CGCG CG^CG
3600 CGCG
Alw44I 2764 GTGCAC G^TGCAC
4338 GTGCAC
ApaLI 2764 GTGCAC G^TGCAC
4338 GTGCAC
Asp718I 3360 GGTACC G^GTACC
4701 GGTACC
AvrII 1240 CCTAGG C^CTAGG
3483 CCTAGG
BalI 1190 TGGCCA TGG^CCA
2528 TGGCCA
BbeI 643 GGCGCC GGCGC^C
4172 GGCGCC
BbrPI 575 CACGTG CAC^GTG
1635 CACGTG
BciVI 2378 GTATCC GTATCC(6/5)
3488 GTATCC
BlnI 1240 CCTAGG C^CTAGG
3483 CCTAGG
BsaBI 1255 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2526 GATGGCCATC
BsaI 980 GGTCTC GGTCTC(1/5)
1104 GGTCTC
BsmBI 968 CGTCTC CGTCTC(1/5)
24 GAGACG
BspHI 4090 TCATGA T^CATGA
4104 TCATGA
BspLU11I 1137 ACATGT A^CATGT
2732 ACATGT
BsrBI 3057 CCGCTC CCGCTC(-3/-3)
2860 GAGCGG
BssSI 1506 CTCGTG CACGAG(-5/-1)
2290 CTCGTG
BstUI 2502 CGCG CG^CG
3600 CGCG
HgaI 443 GACGC GACGC(5/10)
2966 GCGTC
KasI 643 GGCGCC G^GCGCC
4172 GGCGCC
KpnI 3360 GGTACC GGTAC^C
4701 GGTACC
MamI 1255 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2526 GATGGCCATC
MluNI 1190 TGGCCA TGG^CCA
2528 TGGCCA
MscI 1190 TGGCCA TGG^CCA
2528 TGGCCA
MvnI 2502 CGCG CG^CG
3600 CGCG
NarI 643 GGCGCC GG^CGCC
4172 GGCGCC
NcoI 1588 CCATGG C^CATGG
1711 CCATGG
PmaCI 575 CACGTG CAC^GTG
1635 CACGTG
PmlI 575 CACGTG CAC^GTG
1635 CACGTG
RcaI 4090 TCATGA T^CATGA
4104 TCATGA
SapI 1057 GCTCTTC GCTCTTC(1/4)
4519 GCTCTTC
SfoI 643 GGCGCC GGC^GCC
4172 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmaCI XmaI