##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspI AviII BbeI
BcgI BclI BsaHI BseAI BsiWI
BspDI BspEI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI Eco47III EcoO65I EcoRI
EcoRV FbaI FseI FspI Hin1I
HpaI KasI KspI MfeI MluI
MroI MunI NarI NdeI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I RsrII
SacII SapI SexAI SfoI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 2644 AAATTC [GA]^AATT[CT]
ApoI 2644 AAATTC [GA]^AATT[CT]
AvrII 277 CCTAGG C^CTAGG
BbrPI 3285 CACGTG CAC^GTG
BglII 2480 AGATCT A^GATCT
BlnI 277 CCTAGG C^CTAGG
BsaAI 3285 CACGTG [CT]AC^GT[GA]
BsaBI 3387 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1664 GGTCTC GGTCTC(1/5)
BsaWI 3067 TCCGGT [AT]^CCGG[AT]
BsmBI 5528 GAGACG CGTCTC(1/5)
BsmI 986 GAATGC GAATGC(1/-1)
Bsp1407I 4073 TGTACA T^GTACA
BspHI 5169 TCATGA T^CATGA
BsrBI 4698 CCGCTC CCGCTC(-3/-3)
BsrGI 4073 TGTACA T^GTACA
BstAPI 4044 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5181 TTCGAA TT^CGAA
DraIII 3422 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3845 CGGCCG C^GGCCG
EclXI 3845 CGGCCG C^GGCCG
Eco52I 3845 CGGCCG C^GGCCG
HindIII 1653 AAGCTT A^AGCTT
MamI 3387 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2069 GCCGGC GCC^GGC
NgoMIV 2069 GCCGGC G^CCGGC
NspV 5181 TTCGAA TT^CGAA
PmaCI 3285 CACGTG CAC^GTG
PmlI 3285 CACGTG CAC^GTG
PvuI 3152 CGATCG CGAT^CG
RcaI 5169 TCATGA T^CATGA
SalI 5420 GTCGAC G^TCGAC
SbfI 2053 CCTGCAGG CCTGCA^GG
ScaI 2683 AGTACT AGT^ACT
SfiI 3272 GGCCGTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5181 TTCGAA TT^CGAA
Sse8387I 2053 CCTGCAGG CCTGCA^GG
SspBI 4073 TGTACA T^GTACA
XbaI 464 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 4717 GTCTAC GT^[AC][GT]AC
5420 GTCGAC
AccII 1820 CGCG CG^CG
5350 CGCG
AflII 2393 CTTAAG C^TTAAG
2539 CTTAAG
AflIII 1350 ACATGT A^C[GA][CT]GT
4076 ACATGT
AhdI 1349 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4297 GACCCCATGTC
AspEI 1349 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4297 GACCCCATGTC
BalI 1106 TGGCCA TGG^CCA
3369 TGGCCA
BfrI 2393 CTTAAG C^TTAAG
2539 CTTAAG
BglI 3081 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3273 GCCGTCCTGGC
BsiEI 3152 CGATCG CG[GA][CT]^CG
3845 CGGCCG
BspLU11I 1350 ACATGT A^CATGT
4076 ACATGT
BstUI 1820 CGCG CG^CG
5350 CGCG
Eam1105I 1349 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4297 GACCCCATGTC
HgaI 3192 GACGC GACGC(5/10)
3299 GACGC
MluNI 1106 TGGCCA TGG^CCA
3369 TGGCCA
MscI 1106 TGGCCA TGG^CCA
3369 TGGCCA
MvnI 1820 CGCG CG^CG
5350 CGCG
SmaI 1785 CCCGGG CCC^GGG
4368 CCCGGG
SphI 4662 GCATGC GCATG^C
4929 GCATGC
XmaCI 1785 CCCGGG C^CCGGG
4368 CCCGGG
XmaI 1785 CCCGGG C^CCGGG
4368 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp718I AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NheI NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII