##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Cfr10I CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 585 GTAGAC GT^[AC][GT]AC
AcyI 7 GGCGCC G[GA]^CG[CT]C
AflIII 706 ACATGT A^C[GA][CT]GT
AspHI 1007 GAGCTC G[AT]GC[AT]^C
BbeI 7 GGCGCC GGCGC^C
BbrPI 225 CACGTG CAC^GTG
BlpI 897 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 897 GCTCAGC GC^T[ACGT]AGC
BsaAI 225 CACGTG [CT]AC^GT[GA]
BsaHI 7 GGCGCC G[GA]^CG[CT]C
BseRI 891 GAGGAG GAGGAG(10/8)
BsiHKAI 1007 GAGCTC G[AT]GC[AT]^C
BspDI 735 ATCGAT AT^CGAT
BspLU11I 706 ACATGT A^CATGT
BsrDI 748 GCAATG GCAATG(2/0)
BstEII 827 GGTGACC G^GT[ACGT]ACC
BstF5I 288 CATCC GGATG(2/0)
BstPI 827 GGTGACC G^GT[ACGT]ACC
BstXI 224 CCACGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 897 GCTCAGC GC^T[ACGT]AGC
ClaI 735 ATCGAT AT^CGAT
DraIII 1012 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 610 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 322 CCATGG C^C[GA][CT]GG
EarI 644 CTCTTC CTCTTC(1/4)
EcoNI 21 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 827 GGTGACC G^GT[ACGT]ACC
FokI 288 CATCC GGATG(9/13)
HaeII 7 GGCGCC [GA]GCGC^[CT]
HgaI 365 GACGC GACGC(5/10)
Hin1I 7 GGCGCC G[GA]^CG[CT]C
KasI 7 GGCGCC G^GCGCC
Ksp632I 644 CTCTTC CTCTTC(1/4)
MseI 679 TTAA T^TAA
MspA1I 1031 CAGCTG C[AC]G^C[GT]G
NarI 7 GGCGCC GG^CGCC
NcoI 322 CCATGG C^CATGG
PaeR7I 60 CTCGAG C^TCGAG
PmaCI 225 CACGTG CAC^GTG
PmlI 225 CACGTG CAC^GTG
PpuMI 17 AGGACCT [GA]G^G[AT]CC[CT]
PstI 650 CTGCAG CTGCA^G
PvuII 1031 CAGCTG CAG^CTG
SacI 1007 GAGCTC GAGCT^C
SapI 643 GCTCTTC GCTCTTC(1/4)
SexAI 815 ACCTGGT A^CC[AT]GGT
SfaNI 742 GCATC GCATC(5/9)
SfcI 650 CTGCAG C^T[GA][CT]AG
SfoI 7 GGCGCC GGC^GCC
SmlI 60 CTCGAG C^T[CT][GA]AG
Tru9I 679 TTAA T^TAA
XcmI 475 CCACCTGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 60 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
AccII 304 CGCG CG^CG
367 CGCG
AcsI 922 GAATTC [GA]^AATT[CT]
1136 GAATTC
AfaI 704 GTAC GT^AC
710 GTAC
AocI 267 CCTAAGG CC^T[ACGT]AGG
775 CCTCAGG
ApaI 148 GGGCCC GGGCC^C
691 GGGCCC
ApoI 922 GAATTC [GA]^AATT[CT]
1136 GAATTC
AvaI 60 CTCGAG C^[CT]CG[GA]G
666 CCCGAG
BalI 1060 TGGCCA TGG^CCA
1099 TGGCCA
BamHI 351 GGATCC G^GATCC
671 GGATCC
BbsI 297 GAAGAC GAAGAC(2/6)
603 GTCTTC
BcnI 355 CCGGG CC^[GC]GG
689 CCGGG
BglI 396 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1092 GCCCCATTGGC
BpmI 185 CTGGAG CTGGAG(16/14)
934 CTGGAG
BpuAI 297 GAAGAC GAAGAC(2/6)
603 GTCTTC
BsoBI 60 CTCGAG C^[CT]CG[GA]G
666 CCCGAG
BsrI 184 ACTGG ACTGG(1/-1)
553 ACTGG
BstUI 304 CGCG CG^CG
367 CGCG
BstYI 351 GGATCC [GA]^GATC[CT]
671 GGATCC
Bsu36I 267 CCTAAGG CC^T[ACGT]AGG
775 CCTCAGG
CfoI 8 GCGC GCG^C
756 GCGC
DpnII 352 GATC ^GATC
672 GATC
EaeI 1060 TGGCCA [CT]^GGCC[GA]
1099 TGGCCA
Eco81I 267 CCTAAGG CC^T[ACGT]AGG
775 CCTCAGG
EcoRI 922 GAATTC G^AATTC
1136 GAATTC
FauI 540 CCCGC CCCGC(4/6)
568 GCGGG
HapII 355 CCGG C^CGG
689 CCGG
HhaI 8 GCGC GCG^C
756 GCGC
HinP1I 8 GCGC G^CGC
756 GCGC
HpaII 355 CCGG C^CGG
689 CCGG
MaeII 226 ACGT A^CGT
1046 ACGT
MboI 352 GATC ^GATC
672 GATC
MflI 351 GGATCC [GA]^GATC[CT]
671 GGATCC
MluNI 1060 TGGCCA TGG^CCA
1099 TGGCCA
MscI 1060 TGGCCA TGG^CCA
1099 TGGCCA
MslI 1077 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1145 CATCTTGATG
MspI 355 CCGG C^CGG
689 CCGG
MvnI 304 CGCG CG^CG
367 CGCG
NciI 355 CCGGG CC^[GC]GG
689 CCGGG
NdeII 352 GATC ^GATC
672 GATC
NspI 374 ACATGC [GA]CATG^[CT]
706 ACATGT
PflMI 547 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1063 CCAAGTGCTGG
RsaI 704 GTAC GT^AC
710 GTAC
Sau3AI 352 GATC ^GATC
672 GATC
Tsp509I 923 AATT ^AATT
1137 AATT
Van91I 547 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1063 CCAAGTGCTGG
XhoII 351 GGATCC [GA]^GATC[CT]
671 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII EcoT14I
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI StyI TaqI TseI Tsp45I
TspRI TthHB8I