##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AspI AvaI
BbeI BcgI BfrI BglI BsaBI
BsaHI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
Hin1I KasI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3896 GGTACC G^GTACC
AseI 4162 ATTAAT AT^TAAT
AsnI 4162 ATTAAT AT^TAAT
Asp700I 4099 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3896 GGTACC G^GTACC
AviII 4073 TGCGCA TGC^GCA
AvrII 985 CCTAGG C^CTAGG
BalI 1589 TGGCCA TGG^CCA
BbrPI 1581 CACGTG CAC^GTG
BglII 911 AGATCT A^GATCT
BlnI 985 CCTAGG C^CTAGG
BlpI 3806 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3806 GCTGAGC GC^T[ACGT]AGC
BsaAI 1581 CACGTG [CT]AC^GT[GA]
BsaWI 3303 ACCGGA [AT]^CCGG[AT]
BsmBI 5215 GAGACG CGTCTC(1/5)
Bsp1407I 1821 TGTACA T^GTACA
BspLU11I 1791 ACATGT A^CATGT
BspMI 2050 GCAGGT ACCTGC(4/8)
BsrDI 2111 CATTGC GCAATG(2/0)
BsrGI 1821 TGTACA T^GTACA
BstEII 1833 GGTCACC G^GT[ACGT]ACC
BstPI 1833 GGTCACC G^GT[ACGT]ACC
CelII 3806 GCTGAGC GC^T[ACGT]AGC
EaeI 1589 TGGCCA [CT]^GGCC[GA]
EcoO65I 1833 GGTCACC G^GT[ACGT]ACC
EcoRI 43 GAATTC G^AATTC
EcoRV 2413 GATATC GAT^ATC
FauI 2688 CCCGC CCCGC(4/6)
FspI 4073 TGCGCA TGC^GCA
HaeII 793 AGCGCC [GA]GCGC^[CT]
HgaI 3327 GCGTC GACGC(5/10)
KpnI 3896 GGTACC GGTAC^C
MaeII 1582 ACGT A^CGT
MfeI 5472 CAATTG C^AATTG
MluNI 1589 TGGCCA TGG^CCA
MscI 1589 TGGCCA TGG^CCA
MunI 5472 CAATTG C^AATTG
NheI 3033 GCTAGC G^CTAGC
PmaCI 1581 CACGTG CAC^GTG
PmlI 1581 CACGTG CAC^GTG
PshBI 4162 ATTAAT AT^TAAT
SapI 1111 GAAGAGC GCTCTTC(1/4)
SexAI 3961 ACCAGGT A^CC[AT]GGT
SspBI 1821 TGTACA T^GTACA
TaqI 2663 TCGA T^CGA
TthHB8I 2663 TCGA T^CGA
XmnI 4099 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1582 ACGTGT A^C[GA][CT]GT
1791 ACATGT
AocI 561 CCTGAGG CC^T[ACGT]AGG
2100 CCTGAGG
BamHI 1352 GGATCC G^GATCC
2681 GGATCC
BcnI 2778 CCCGG CC^[GC]GG
5322 CCCGG
BssSI 3624 CTCGTG CACGAG(-5/-1)
5262 CTCGTG
Bsu36I 561 CCTGAGG CC^T[ACGT]AGG
2100 CCTGAGG
DraIII 1444 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2222 CACACAGTG
Eco81I 561 CCTGAGG CC^T[ACGT]AGG
2100 CCTGAGG
HincII 3013 GTTAAC GT[CT]^[GA]AC
3192 GTTAAC
HindII 3013 GTTAAC GT[CT]^[GA]AC
3192 GTTAAC
HpaI 3013 GTTAAC GTT^AAC
3192 GTTAAC
NciI 2778 CCCGG CC^[GC]GG
5322 CCCGG
SphI 330 GCATGC GCATG^C
3101 GCATGC
XbaI 908 TCTAGA T^CTAGA
1675 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BciVI BclI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII