##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AclI AgeI ApaI AscI AseI
AsnI Asp700I AviII BamHI BbeI
BbrPI BciVI BsaBI BsiWI BsmBI
BspDI BspLU11I BssHII BstBI BstEII
BstPI ClaI CpoI EcoO65I EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI MamI MluI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2770 GGTACC G^GTACC
AhdI 2355 GACCTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2980 GTGCAC G^TGCAC
ApaLI 2980 GTGCAC G^TGCAC
Asp718I 2770 GGTACC G^GTACC
AspEI 2355 GACCTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1985 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2774 CCTAGG C^CTAGG
BclI 2735 TGATCA T^GATCA
BglII 4958 AGATCT A^GATCT
BlnI 2774 CCTAGG C^CTAGG
Bst1107I 4868 GTATAC GTA^TAC
BstZ17I 4868 GTATAC GTA^TAC
DraI 2933 TTTAAA TTT^AAA
Eam1105I 2355 GACCTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5177 GAATTC G^AATTC
FbaI 2735 TGATCA T^GATCA
KpnI 2770 GGTACC GGTAC^C
KspI 1189 CCGCGG CCGC^GG
MfeI 5373 CAATTG C^AATTG
MunI 5373 CAATTG C^AATTG
NaeI 1242 GCCGGC GCC^GGC
NgoMIV 1242 GCCGGC G^CCGGC
PflFI 1985 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 2818 CAGCTG CAG^CTG
SacII 1189 CCGCGG CCGC^GG
SapI 2370 GAAGAGC GCTCTTC(1/4)
Tth111I 1985 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2706 GACGTC GACGT^C
4973 GACGTC
AccIII 2948 TCCGGA T^CCGGA
4246 TCCGGA
AflIII 1795 ACGTGT A^C[GA][CT]GT
3503 ACACGT
AlwI 3089 GGATC GGATC(4/5)
4129 GGATC
BcgI 50 CGAGTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5219 GCACAGAAGTCG
BglI 958 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1371 GCCAGCAGGGC
BlpI 164 GCTGAGC GC^T[ACGT]AGC
4225 GCTAAGC
Bpu1102I 164 GCTGAGC GC^T[ACGT]AGC
4225 GCTAAGC
BsaAI 2232 TACGTG [CT]AC^GT[GA]
2493 TACGTG
BsaI 2150 GAGACC GGTCTC(1/5)
2410 GAGACC
BseAI 2948 TCCGGA T^CCGGA
4246 TCCGGA
BsmI 4211 GAATGC GAATGC(1/-1)
3423 GCATTC
BspEI 2948 TCCGGA T^CCGGA
4246 TCCGGA
BsrDI 4013 CATTGC GCAATG(2/0)
4268 GCAATG
BssSI 669 CACGAG CACGAG(-5/-1)
1211 CACGAG
BstAPI 2873 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3517 GCATGAACTGC
BstXI 1843 CCAGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3209 CCAGAAGGATGG
BstYI 3089 GGATCT [GA]^GATC[CT]
4958 AGATCT
CelII 164 GCTGAGC GC^T[ACGT]AGC
4225 GCTAAGC
DraIII 2427 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2974 CACAGGGTG
EagI 985 CGGCCG C^GGCCG
2022 CGGCCG
EclXI 985 CGGCCG C^GGCCG
2022 CGGCCG
Eco52I 985 CGGCCG C^GGCCG
2022 CGGCCG
MflI 3089 GGATCT [GA]^GATC[CT]
4958 AGATCT
MroI 2948 TCCGGA T^CCGGA
4246 TCCGGA
PstI 2757 CTGCAG CTGCA^G
3045 CTGCAG
SacI 1667 GAGCTC GAGCT^C
3341 GAGCTC
SalI 1611 GTCGAC G^TCGAC
4852 GTCGAC
StuI 1033 AGGCCT AGG^CCT
4274 AGGCCT
XhoII 3089 GGATCT [GA]^GATC[CT]
4958 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AluI AlwNI AocI
Aor51HI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmaCI XmaI