##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccI AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AviII BbeI BbrPI BcgI BciVI
BclI BfrI BglII BsiWI BsmI
Bsp1407I BspDI BspHI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII Eco47III EcoRV EcoT22I FbaI
FspI HindIII HpaI KasI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 1858 TCCGGA T^CCGGA
AhdI 5042 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5042 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5205 CCTAGG C^CTAGG
BamHI 5209 GGATCC G^GATCC
BlnI 5205 CCTAGG C^CTAGG
BsaAI 2387 CACGTA [CT]AC^GT[GA]
BsaBI 449 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1858 TCCGGA [AT]^CCGG[AT]
BseAI 1858 TCCGGA T^CCGGA
BspEI 1858 TCCGGA T^CCGGA
BssSI 4108 CACGAG CACGAG(-5/-1)
DrdI 1114 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5042 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 3569 GGCCGGCC GGCCGG^CC
HincII 782 GTCAAC GT[CT]^[GA]AC
HindII 782 GTCAAC GT[CT]^[GA]AC
KspI 3565 CCGCGG CCGC^GG
MamI 449 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1858 TCCGGA T^CCGGA
PaeR7I 1803 CTCGAG C^TCGAG
SacII 3565 CCGCGG CCGC^GG
SbfI 4296 CCTGCAGG CCTGCA^GG
SexAI 2743 ACCAGGT A^CC[AT]GGT
Sse8387I 4296 CCTGCAGG CCTGCA^GG
SspI 5416 AATATT AAT^ATT
XhoI 1803 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 1120 GACGTC GACGT^C
1977 GACGTC
AflIII 2778 ACATGT A^C[GA][CT]GT
5088 ACATGT
Asp700I 321 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1636 GAACCTGTTC
AspI 1994 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3848 GACAGGGTC
BlpI 248 GCTGAGC GC^T[ACGT]AGC
4077 GCTCAGC
Bpu1102I 248 GCTGAGC GC^T[ACGT]AGC
4077 GCTCAGC
BsiEI 1758 CGGCCG CG[GA][CT]^CG
3568 CGGCCG
BsmBI 1979 CGTCTC CGTCTC(1/5)
2769 GAGACG
BspLU11I 2778 ACATGT A^CATGT
5088 ACATGT
BstEII 3853 GGTCACC G^GT[ACGT]ACC
4721 GGTCACC
BstPI 3853 GGTCACC G^GT[ACGT]ACC
4721 GGTCACC
BstYI 237 AGATCC [GA]^GATC[CT]
5209 GGATCC
CelII 248 GCTGAGC GC^T[ACGT]AGC
4077 GCTCAGC
DraI 4887 TTTAAA TTT^AAA
5376 TTTAAA
EagI 1758 CGGCCG C^GGCCG
3568 CGGCCG
EclXI 1758 CGGCCG C^GGCCG
3568 CGGCCG
Eco52I 1758 CGGCCG C^GGCCG
3568 CGGCCG
EcoO65I 3853 GGTCACC G^GT[ACGT]ACC
4721 GGTCACC
EcoRI 45 GAATTC G^AATTC
998 GAATTC
MflI 237 AGATCC [GA]^GATC[CT]
5209 GGATCC
PflFI 1994 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3848 GACAGGGTC
PshAI 1699 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5087 GACATGTGTC
SphI 1039 GCATGC GCATG^C
1836 GCATGC
Tth111I 1994 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3848 GACAGGGTC
XhoII 237 AGATCC [GA]^GATC[CT]
5209 GGATCC
XmnI 321 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1636 GAACCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI