##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccII AclI AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AspEI AviII BamHI BbrPI BcgI
BsiEI BsiWI BspDI BsrBI BsrFI
BssHII Bst1107I BstBI BstUI BstZ17I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
FseI FspI KspI MfeI MluI
MunI MvnI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 514 GTCTAC GT^[AC][GT]AC
AccIII 214 TCCGGA T^CCGGA
AflII 1893 CTTAAG C^TTAAG
AocI 74 CCTTAGG CC^T[ACGT]AGG
ApaI 5587 GGGCCC GGGCC^C
AspI 1458 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1496 GGCGCC GGCGC^C
BciVI 5713 GGATAC GTATCC(6/5)
BclI 2962 TGATCA T^GATCA
BfrI 1893 CTTAAG C^TTAAG
BglI 1499 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4848 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4848 GCTCAGC GC^T[ACGT]AGC
BsaAI 4620 TACGTA [CT]AC^GT[GA]
BsaBI 4804 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 214 TCCGGA [AT]^CCGG[AT]
BseAI 214 TCCGGA T^CCGGA
BsmBI 1456 GAGACG CGTCTC(1/5)
BspEI 214 TCCGGA T^CCGGA
BspMI 2033 ACCTGC ACCTGC(4/8)
BssSI 3672 CACGAG CACGAG(-5/-1)
BstAPI 1109 GCAAGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1171 GGTTACC G^GT[ACGT]ACC
BstPI 1171 GGTTACC G^GT[ACGT]ACC
BstXI 1695 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 74 CCTTAGG CC^T[ACGT]AGG
CelII 4848 GCTCAGC GC^T[ACGT]AGC
CfoI 1497 GCGC GCG^C
DrdI 1200 GACATTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 74 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1171 GGTTACC G^GT[ACGT]ACC
FbaI 2962 TGATCA T^GATCA
HaeII 1496 GGCGCC [GA]GCGC^[CT]
HgaI 5352 GCGTC GACGC(5/10)
HhaI 1497 GCGC GCG^C
HinP1I 1497 GCGC G^CGC
KasI 1496 GGCGCC G^GCGCC
MamI 4804 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 214 TCCGGA T^CCGGA
MspA1I 5327 CAGCTG C[AC]G^C[GT]G
NarI 1496 GGCGCC GG^CGCC
NcoI 2062 CCATGG C^CATGG
NheI 3808 GCTAGC G^CTAGC
PflFI 1458 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2385 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 5327 CAGCTG CAG^CTG
SacI 5525 GAGCTC GAGCT^C
SfoI 1496 GGCGCC GGC^GCC
SmaI 1528 CCCGGG CCC^GGG
SnaBI 4620 TACGTA TAC^GTA
SphI 3046 GCATGC GCATG^C
StuI 3423 AGGCCT AGG^CCT
Tth111I 1458 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1179 TCTAGA T^CTAGA
XcmI 2077 CCAAAACGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1528 CCCGGG C^CCGGG
XmaI 1528 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2921 GGTACC G^GTACC
4144 GGTACC
AcyI 1496 GGCGCC G[GA]^CG[CT]C
5351 GGCGTC
Asp718I 2921 GGTACC G^GTACC
4144 GGTACC
AvaI 1528 CCCGGG C^[CT]CG[GA]G
1560 CCCGAG
AvrII 345 CCTAGG C^CTAGG
1950 CCTAGG
BbvCI 1685 CCTCAGC CCTCAGC(-5/-2)
3260 CCTCAGC
BglII 3171 AGATCT A^GATCT
4128 AGATCT
BlnI 345 CCTAGG C^CTAGG
1950 CCTAGG
BsaHI 1496 GGCGCC G[GA]^CG[CT]C
5351 GGCGTC
BsaI 3689 GAGACC GGTCTC(1/5)
3361 GGTCTC
BsoBI 1528 CCCGGG C^[CT]CG[GA]G
1560 CCCGAG
BspHI 1306 TCATGA T^CATGA
3751 TCATGA
BspLU11I 179 ACATGT A^CATGT
358 ACATGT
DraII 2385 AGGTCCC [GA]G^G[ACGT]CC[CT]
5586 AGGGCCC
DraIII 2542 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3177 CACTCAGTG
EarI 4569 GAAGAG CTCTTC(1/4)
5736 GAAGAG
EcoO109I 2385 AGGTCCC [GA]G^G[ACGT]CC[CT]
5586 AGGGCCC
EcoRI 5211 GAATTC G^AATTC
5277 GAATTC
FauI 1584 CCCGC CCCGC(4/6)
1681 CCCGC
Hin1I 1496 GGCGCC G[GA]^CG[CT]C
5351 GGCGTC
HpaI 795 GTTAAC GTT^AAC
2263 GTTAAC
KpnI 2921 GGTACC GGTAC^C
4144 GGTACC
Ksp632I 4569 GAAGAG CTCTTC(1/4)
5736 GAAGAG
NdeI 985 CATATG CA^TATG
3694 CATATG
PflMI 227 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
640 CCAAAGGATGG
RcaI 1306 TCATGA T^CATGA
3751 TCATGA
SpeI 1314 ACTAGT A^CTAGT
4291 ACTAGT
Van91I 227 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
640 CCAAAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EaeI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI