##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI AhdI Aor51HI AscI AspEI
AviII AvrII BalI BamHI BbeI
BcgI BciVI BglII BlnI BsaBI
BsaHI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
FspI Hin1I HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SspBI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 1805 GGGCCC GGGCC^C
BbrPI 2858 CACGTG CAC^GTG
BclI 3337 TGATCA T^GATCA
BsaWI 2245 ACCGGA [AT]^CCGG[AT]
BsmBI 1051 GAGACG CGTCTC(1/5)
BspLU11I 3530 ACATGT A^CATGT
BsrBI 2394 GAGCGG CCGCTC(-3/-3)
DraIII 613 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2987 TGGCCG [CT]^GGCC[GA]
EcoRV 714 GATATC GAT^ATC
FbaI 3337 TGATCA T^GATCA
NcoI 4748 CCATGG C^CATGG
NdeI 2353 CATATG CA^TATG
PmaCI 2858 CACGTG CAC^GTG
PmlI 2858 CACGTG CAC^GTG
SgrAI 2325 CACCGGCG C[GA]^CCGG[CT]G
SphI 3055 GCATGC GCATG^C
TaqI 1085 TCGA T^CGA
TthHB8I 1085 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 454 GGTACC G^GTACC
1346 GGTACC
AflII 3944 CTTAAG C^TTAAG
4629 CTTAAG
AlwNI 115 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2229 CAGCACCTG
AseI 3428 ATTAAT AT^TAAT
3468 ATTAAT
AsnI 3428 ATTAAT AT^TAAT
3468 ATTAAT
Asp718I 454 GGTACC G^GTACC
1346 GGTACC
AvaI 3073 CCCGAG C^[CT]CG[GA]G
4539 CTCGGG
BbvCI 34 CCTCAGC CCTCAGC(-5/-2)
1113 CCTCAGC
BcnI 1252 CCGGG CC^[GC]GG
2260 CCGGG
BfrI 3944 CTTAAG C^TTAAG
4629 CTTAAG
BsoBI 3073 CCCGAG C^[CT]CG[GA]G
4539 CTCGGG
BsrDI 3677 CATTGC GCAATG(2/0)
591 GCAATG
BsrFI 2326 ACCGGC [GA]^CCGG[CT]
2488 ACCGGC
Cfr10I 2326 ACCGGC [GA]^CCGG[CT]
2488 ACCGGC
DrdI 1686 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3999 GACTGCTGTGTC
EcoNI 268 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
424 CCTGGGGAAGG
HaeII 361 GGCGCT [GA]GCGC^[CT]
1757 GGCGCT
HincII 3298 GTCAAC GT[CT]^[GA]AC
3592 GTTGAC
HindII 3298 GTCAAC GT[CT]^[GA]AC
3592 GTTGAC
KpnI 454 GGTACC GGTAC^C
1346 GGTACC
NciI 1252 CCGGG CC^[GC]GG
2260 CCGGG
PshAI 78 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3752 GACCCTTGTC
PshBI 3428 ATTAAT AT^TAAT
3468 ATTAAT
SacI 173 GAGCTC GAGCT^C
1976 GAGCTC
SapI 170 GAAGAGC GCTCTTC(1/4)
2210 GAAGAGC
XbaI 768 TCTAGA T^CTAGA
3378 TCTAGA
XcmI 1208 CCAGAGTGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4594 CCAGCATTCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AocI ApaLI
ApoI Asp700I AspHI AspI AvaII
BanI BanII BbsI BbvI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NsiI NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I Van91I XhoII XmnI