##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII AclI AflII AhdI AscI
AseI AsnI AspEI BclI BfrI
BglII BspDI Bst1107I BstZ17I ClaI
CpoI Eam1105I EcoRI EcoRV EcoT22I
FbaI HindIII HpaI MluI NcoI
NotI NruI NsiI PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SexAI SfiI SgrAI SpeI SphI
SwaI TfiI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 3624 GGTACC G^GTACC
AccIII 3730 TCCGGA T^CCGGA
AgeI 5095 ACCGGT A^CCGGT
AocI 2192 CCTGAGG CC^T[ACGT]AGG
Asp700I 5004 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3624 GGTACC G^GTACC
AspI 4185 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2662 CCTAGG C^CTAGG
BbrPI 3636 CACGTG CAC^GTG
BlnI 2662 CCTAGG C^CTAGG
BseAI 3730 TCCGGA T^CCGGA
BsiWI 1225 CGTACG C^GTACG
BsmI 1483 GAATGC GAATGC(1/-1)
BspEI 3730 TCCGGA T^CCGGA
BspHI 4853 TCATGA T^CATGA
BspLU11I 3465 ACATGT A^CATGT
BstBI 1480 TTCGAA TT^CGAA
Bsu36I 2192 CCTGAGG CC^T[ACGT]AGG
DraI 5241 TTTAAA TTT^AAA
DrdI 2180 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2192 CCTGAGG CC^T[ACGT]AGG
FseI 101 GGCCGGCC GGCCGG^CC
KpnI 3624 GGTACC GGTAC^C
MfeI 1185 CAATTG C^AATTG
MroI 3730 TCCGGA T^CCGGA
MunI 1185 CAATTG C^AATTG
NheI 3102 GCTAGC G^CTAGC
NspV 1480 TTCGAA TT^CGAA
PflFI 4185 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5095 ACCGGT A^CCGGT
PmaCI 3636 CACGTG CAC^GTG
PmlI 3636 CACGTG CAC^GTG
RcaI 4853 TCATGA T^CATGA
SalI 4182 GTCGAC G^TCGAC
SbfI 2327 CCTGCAGG CCTGCA^GG
SfuI 1480 TTCGAA TT^CGAA
SnaBI 262 TACGTA TAC^GTA
Sse8387I 2327 CCTGCAGG CCTGCA^GG
SspI 5137 AATATT AAT^ATT
Tth111I 4185 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 5004 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BamHI 3112 GGATCC G^GATCC
4695 GGATCC
BcgI 1545 GCAGCGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2260 GCAGGGAGCTCG
BciVI 1330 GGATAC GTATCC(6/5)
925 GTATCC
Bsp1407I 3256 TGTACA T^GTACA
4706 TGTACA
BsrDI 806 CATTGC GCAATG(2/0)
1744 GCAATG
BsrGI 3256 TGTACA T^GTACA
4706 TGTACA
BssHII 227 GCGCGC G^CGCGC
2843 GCGCGC
BssSI 2136 CACGAG CACGAG(-5/-1)
2279 CTCGTG
BstXI 959 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2770 CCACCAAGGTGG
DraIII 89 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2202 CACACTGTG
HincII 4182 GTCGAC GT[CT]^[GA]AC
4845 GTCAAC
HindII 4182 GTCGAC GT[CT]^[GA]AC
4845 GTCAAC
MseI 4753 TTAA T^TAA
5242 TTAA
NaeI 102 GCCGGC GCC^GGC
2805 GCCGGC
NdeI 337 CATATG CA^TATG
4019 CATATG
NgoMIV 102 GCCGGC G^CCGGC
2805 GCCGGC
PpuMI 1003 GGGTCCC [GA]G^G[AT]CC[CT]
3832 GGGACCT
PshAI 4410 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4539 GACCATCGTC
SacI 942 GAGCTC GAGCT^C
2265 GAGCTC
SapI 390 GAAGAGC GCTCTTC(1/4)
231 GCTCTTC
ScaI 1832 AGTACT AGT^ACT
3274 AGTACT
SspBI 3256 TGTACA T^GTACA
4706 TGTACA
Tru9I 4753 TTAA T^TAA
5242 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AspHI AvaI AvaII AviII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI