##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
BclI BfrI BglII BspDI Bst1107I
BstZ17I ClaI CpoI Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MluI NcoI NotI
NruI NsiI PacI PaeR7I PmeI
PshBI Psp1406I PvuI RsrII SexAI
SfiI SgrAI SpeI SwaI TfiI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 3636 TCCGGA T^CCGGA
AgeI 5001 ACCGGT A^CCGGT
AocI 2093 CCTGAGG CC^T[ACGT]AGG
Asp700I 4910 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4091 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2563 CCTAGG C^CTAGG
BbrPI 3542 CACGTG CAC^GTG
BlnI 2563 CCTAGG C^CTAGG
BseAI 3636 TCCGGA T^CCGGA
BsiWI 968 CGTACG C^GTACG
BsmI 1226 GAATGC GAATGC(1/-1)
BspEI 3636 TCCGGA T^CCGGA
BspHI 4759 TCATGA T^CATGA
BspLU11I 3366 ACATGT A^CATGT
BssHII 2744 GCGCGC G^CGCGC
BstBI 1223 TTCGAA TT^CGAA
Bsu36I 2093 CCTGAGG CC^T[ACGT]AGG
DraI 5147 TTTAAA TTT^AAA
DraIII 2103 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2081 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2093 CCTGAGG CC^T[ACGT]AGG
MfeI 928 CAATTG C^AATTG
MroI 3636 TCCGGA T^CCGGA
MunI 928 CAATTG C^AATTG
NaeI 2706 GCCGGC GCC^GGC
NgoMIV 2706 GCCGGC G^CCGGC
NheI 3003 GCTAGC G^CTAGC
NspV 1223 TTCGAA TT^CGAA
PflFI 4091 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5001 ACCGGT A^CCGGT
PmaCI 3542 CACGTG CAC^GTG
PmlI 3542 CACGTG CAC^GTG
RcaI 4759 TCATGA T^CATGA
SalI 4088 GTCGAC G^TCGAC
SapI 133 GAAGAGC GCTCTTC(1/4)
SbfI 2228 CCTGCAGG CCTGCA^GG
SfuI 1223 TTCGAA TT^CGAA
SnaBI 5 TACGTA TAC^GTA
SphI 1646 GCATGC GCATG^C
Sse8387I 2228 CCTGCAGG CCTGCA^GG
SspI 5043 AATATT AAT^ATT
Tth111I 4091 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4910 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 4391 AAATTT [GA]^AATT[CT]
4520 AAATTT
ApoI 4391 AAATTT [GA]^AATT[CT]
4520 AAATTT
AviII 2221 TGCGCA TGC^GCA
4924 TGCGCA
BbvCI 5260 CCTCAGC CCTCAGC(-5/-2)
4292 GCTGAGG
BciVI 1073 GGATAC GTATCC(6/5)
668 GTATCC
Bsp1407I 3157 TGTACA T^GTACA
4612 TGTACA
BsrDI 549 CATTGC GCAATG(2/0)
1487 GCAATG
BsrGI 3157 TGTACA T^GTACA
4612 TGTACA
BssSI 2037 CACGAG CACGAG(-5/-1)
2180 CTCGTG
FspI 2221 TGCGCA TGC^GCA
4924 TGCGCA
HincII 4088 GTCGAC GT[CT]^[GA]AC
4751 GTCAAC
HindII 4088 GTCGAC GT[CT]^[GA]AC
4751 GTCAAC
KspI 2245 CCGCGG CCGC^GG
2505 CCGCGG
MseI 4659 TTAA T^TAA
5148 TTAA
NdeI 80 CATATG CA^TATG
3925 CATATG
PshAI 4316 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4445 GACCATCGTC
SacI 685 GAGCTC GAGCT^C
2166 GAGCTC
SacII 2245 CCGCGG CCGC^GG
2505 CCGCGG
ScaI 1576 AGTACT AGT^ACT
3175 AGTACT
SspBI 3157 TGTACA T^GTACA
4612 TGTACA
Tru9I 4659 TTAA T^TAA
5148 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI