##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI AspI AviII BbeI
BbrPI BciVI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FspI Hin1I HindIII
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI ScaI
SfoI SfuI SgrAI SpeI SphI
SspBI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 4037 GTCTAC GT^[AC][GT]AC
AccII 5203 CGCG CG^CG
AflII 2447 CTTAAG C^TTAAG
Alw44I 1980 GTGCAC G^TGCAC
AocI 1486 CCTCAGG CC^T[ACGT]AGG
ApaLI 1980 GTGCAC G^TGCAC
AseI 4500 ATTAAT AT^TAAT
AsnI 4500 ATTAAT AT^TAAT
AvrII 376 CCTAGG C^CTAGG
BamHI 924 GGATCC G^GATCC
BcgI 1048 CGAAGCATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5103 TGATCA T^GATCA
BfrI 2447 CTTAAG C^TTAAG
BlnI 376 CCTAGG C^CTAGG
BsaAI 4854 TACGTA [CT]AC^GT[GA]
BspLU11I 4327 ACATGT A^CATGT
BsrDI 5194 GCAATG GCAATG(2/0)
BstUI 5203 CGCG CG^CG
Bsu36I 1486 CCTCAGG CC^T[ACGT]AGG
DraI 5303 TTTAAA TTT^AAA
DraIII 1999 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1037 GACTGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1486 CCTCAGG CC^T[ACGT]AGG
EcoT22I 5087 ATGCAT ATGCA^T
FbaI 5103 TGATCA T^GATCA
FseI 3297 GGCCGGCC GGCCGG^CC
HaeII 4344 GGCGCT [GA]GCGC^[CT]
MvnI 5203 CGCG CG^CG
NdeI 4143 CATATG CA^TATG
NheI 2686 GCTAGC G^CTAGC
NsiI 5087 ATGCAT ATGCA^T
PaeR7I 5262 CTCGAG C^TCGAG
PpuMI 1352 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 4500 ATTAAT AT^TAAT
SapI 4939 GCTCTTC GCTCTTC(1/4)
SbfI 2601 CCTGCAGG CCTGCA^GG
SfiI 1008 GGCCCAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 4854 TACGTA TAC^GTA
Sse8387I 2601 CCTGCAGG CCTGCA^GG
StuI 987 AGGCCT AGG^CCT
XhoI 5262 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AflIII 4152 ACGTGT A^C[GA][CT]GT
4327 ACATGT
BspHI 865 TCATGA T^CATGA
3262 TCATGA
BsrBI 2315 CCGCTC CCGCTC(-3/-3)
5411 GAGCGG
BstAPI 1973 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3626 GCAGCTCATGC
HgaI 4035 GCGTC GACGC(5/10)
5204 GCGTC
HpaI 627 GTTAAC GTT^AAC
3772 GTTAAC
NaeI 1014 GCCGGC GCC^GGC
3298 GCCGGC
NcoI 909 CCATGG C^CATGG
4243 CCATGG
NgoMIV 1014 GCCGGC G^CCGGC
3298 GCCGGC
RcaI 865 TCATGA T^CATGA
3262 TCATGA
SexAI 1326 ACCTGGT A^CC[AT]GGT
2194 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI