##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BcgI BfrI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 5161 GTCTAC GT^[AC][GT]AC
AclI 2758 AACGTT AA^CGTT
AcyI 3558 GGCGCC G[GA]^CG[CT]C
Alw44I 4984 GTGCAC G^TGCAC
AlwI 3686 GGATC GGATC(4/5)
AlwNI 4266 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 4984 GTGCAC G^TGCAC
Asp700I 4646 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3558 GGCGCC GGCGC^C
BciVI 4474 GGATAC GTATCC(6/5)
BglI 3621 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5257 AGATCT A^GATCT
BsaHI 3558 GGCGCC G[GA]^CG[CT]C
BspHI 2763 TCATGA T^CATGA
BsrFI 1990 ACCGGC [GA]^CCGG[CT]
BstAPI 5229 GCAAGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1990 ACCGGC [GA]^CCGG[CT]
DraI 4778 TTTAAA TTT^AAA
DraIII 2861 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 5088 GAAGAG CTCTTC(1/4)
HaeII 3558 GGCGCC [GA]GCGC^[CT]
HgaI 4031 GACGC GACGC(5/10)
Hin1I 3558 GGCGCC G[GA]^CG[CT]C
HpaI 4105 GTTAAC GTT^AAC
KasI 3558 GGCGCC G^GCGCC
Ksp632I 5088 GAAGAG CTCTTC(1/4)
NarI 3558 GGCGCC GG^CGCC
NdeI 4073 CATATG CA^TATG
NheI 3347 GCTAGC G^CTAGC
PflFI 2494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2758 AACGTT AA^CGTT
RcaI 2763 TCATGA T^CATGA
SacI 4404 GAGCTC GAGCT^C
SfiI 3620 GGCCCCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3558 GGCGCC GGC^GCC
SgrAI 1989 CACCGGCG C[GA]^CCGG[CT]G
Tth111I 2494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4646 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 86 AAATTC [GA]^AATT[CT]
4613 AAATTC
AocI 2924 CCTGAGG CC^T[ACGT]AGG
3549 CCTCAGG
ApoI 86 AAATTC [GA]^AATT[CT]
4613 AAATTC
BbrPI 640 CACGTG CAC^GTG
2410 CACGTG
BbsI 280 GTCTTC GAAGAC(2/6)
4432 GTCTTC
BbvCI 2734 CCTCAGC CCTCAGC(-5/-2)
5354 GCTGAGG
BpmI 3980 CTGGAG CTGGAG(16/14)
4496 CTGGAG
BpuAI 280 GTCTTC GAAGAC(2/6)
4432 GTCTTC
BsaAI 640 CACGTG [CT]AC^GT[GA]
2410 CACGTG
BspLU11I 4398 ACATGT A^CATGT
4656 ACATGT
BsrBI 180 CCGCTC CCGCTC(-3/-3)
2675 GAGCGG
BsrDI 40 CATTGC GCAATG(2/0)
4924 CATTGC
BstEII 1986 GGTCACC G^GT[ACGT]ACC
3039 GGTCACC
BstPI 1986 GGTCACC G^GT[ACGT]ACC
3039 GGTCACC
BstYI 3686 GGATCT [GA]^GATC[CT]
5257 AGATCT
Bsu36I 2924 CCTGAGG CC^T[ACGT]AGG
3549 CCTCAGG
Eco81I 2924 CCTGAGG CC^T[ACGT]AGG
3549 CCTCAGG
EcoO65I 1986 GGTCACC G^GT[ACGT]ACC
3039 GGTCACC
MflI 3686 GGATCT [GA]^GATC[CT]
5257 AGATCT
PmaCI 640 CACGTG CAC^GTG
2410 CACGTG
PmlI 640 CACGTG CAC^GTG
2410 CACGTG
PpuMI 2658 GGGACCC [GA]G^G[AT]CC[CT]
3011 GGGTCCC
PshAI 4411 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4942 GACACAAGTC
StuI 105 AGGCCT AGG^CCT
4972 AGGCCT
TaqI 4183 TCGA T^CGA
5169 TCGA
TthHB8I 4183 TCGA T^CGA
5169 TCGA
XcmI 2314 CCACCTGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4323 CCAACTTCCAGGTGG
XhoII 3686 GGATCT [GA]^GATC[CT]
5257 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AflIII AluI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbvI BclI BcnI BfaI
BmyI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I