##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Aor51HI AscI AspEI AspI
BciVI BclI BfrI BsaBI BsaWI
BseAI Bsp1407I BspDI BspEI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI Eam1105I Eco47III EcoO65I EcoRV
FbaI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3312 GACGTC GACGT^C
Acc65I 3478 GGTACC G^GTACC
AccII 1512 CGCG CG^CG
AflIII 1630 ACATGT A^C[GA][CT]GT
Alw44I 1828 GTGCAC G^TGCAC
AocI 4003 CCTGAGG CC^T[ACGT]AGG
ApaLI 1828 GTGCAC G^TGCAC
AseI 389 ATTAAT AT^TAAT
AsnI 389 ATTAAT AT^TAAT
Asp700I 3947 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3478 GGTACC G^GTACC
BbrPI 4199 CACGTG CAC^GTG
BbvCI 1259 CCTCAGC CCTCAGC(-5/-2)
BsaAI 4199 CACGTG [CT]AC^GT[GA]
BsiWI 3442 CGTACG C^GTACG
BsmBI 3037 GAGACG CGTCTC(1/5)
BsmI 4431 GCATTC GAATGC(1/-1)
BspHI 4243 TCATGA T^CATGA
BspLU11I 1630 ACATGT A^CATGT
BstUI 1512 CGCG CG^CG
BstXI 1546 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4003 CCTGAGG CC^T[ACGT]AGG
DrdI 4669 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4003 CCTGAGG CC^T[ACGT]AGG
EcoRI 5004 GAATTC G^AATTC
EcoT22I 4772 ATGCAT ATGCA^T
FseI 1060 GGCCGGCC GGCCGG^CC
HindIII 1364 AAGCTT A^AGCTT
HpaI 3652 GTTAAC GTT^AAC
KpnI 3478 GGTACC GGTAC^C
MvnI 1512 CGCG CG^CG
NsiI 4772 ATGCAT ATGCA^T
PaeR7I 4115 CTCGAG C^TCGAG
PmaCI 4199 CACGTG CAC^GTG
PmlI 4199 CACGTG CAC^GTG
PshBI 389 ATTAAT AT^TAAT
RcaI 4243 TCATGA T^CATGA
SbfI 3165 CCTGCAGG CCTGCA^GG
ScaI 4569 AGTACT AGT^ACT
SexAI 3819 ACCTGGT A^CC[AT]GGT
SphI 3562 GCATGC GCATG^C
Sse8387I 3165 CCTGCAGG CCTGCA^GG
StuI 4165 AGGCCT AGG^CCT
XhoI 4115 CTCGAG C^TCGAG
XmnI 3947 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 45 AAATTC [GA]^AATT[CT]
5004 GAATTC
ApoI 45 AAATTC [GA]^AATT[CT]
5004 GAATTC
AvrII 243 CCTAGG C^CTAGG
3330 CCTAGG
BalI 1423 TGGCCA TGG^CCA
3696 TGGCCA
BamHI 3422 GGATCC G^GATCC
4375 GGATCC
BglII 3198 AGATCT A^GATCT
4449 AGATCT
BlnI 243 CCTAGG C^CTAGG
3330 CCTAGG
BsiEI 3288 CGGCCG CG[GA][CT]^CG
3882 CGGCCG
BsrI 3174 ACTGG ACTGG(1/-1)
4848 CCAGT
EagI 3288 CGGCCG C^GGCCG
3882 CGGCCG
EclXI 3288 CGGCCG C^GGCCG
3882 CGGCCG
Eco52I 3288 CGGCCG C^GGCCG
3882 CGGCCG
EcoNI 19 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
175 CCTCTGGAAGG
MluNI 1423 TGGCCA TGG^CCA
3696 TGGCCA
MscI 1423 TGGCCA TGG^CCA
3696 TGGCCA
NspI 1630 ACATGT [GA]CATG^[CT]
3562 GCATGC
PflMI 1438 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1716 CCACTTCCTGG
Van91I 1438 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1716 CCACTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
AviII BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII XmaCI XmaI