##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AhdI AscI
Asp718I AspEI BamHI BbrPI BclI
BcnI BsaAI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
KpnI KspI MroI NaeI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 47 GTAGAC GT^[AC][GT]AC
AclI 5864 AACGTT AA^CGTT
AgeI 121 ACCGGT A^CCGGT
Alw44I 5511 GTGCAC G^TGCAC
Aor51HI 2863 AGCGCT AGC^GCT
ApaLI 5511 GTGCAC G^TGCAC
AviII 1861 TGCGCA TGC^GCA
AvrII 3911 CCTAGG C^CTAGG
BalI 4392 TGGCCA TGG^CCA
BbeI 508 GGCGCC GGCGC^C
BcgI 4489 GCACAAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4394 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3911 CCTAGG C^CTAGG
BsaBI 2041 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 121 ACCGGT [AT]^CCGG[AT]
BsrDI 4544 CATTGC GCAATG(2/0)
BstEII 793 GGTGACC G^GT[ACGT]ACC
BstPI 793 GGTGACC G^GT[ACGT]ACC
DrdI 1156 GACAGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2863 AGCGCT AGC^GCT
EcoO65I 793 GGTGACC G^GT[ACGT]ACC
FspI 1861 TGCGCA TGC^GCA
HpaI 4986 GTTAAC GTT^AAC
KasI 508 GGCGCC G^GCGCC
MamI 2041 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 735 ACGCGT A^CGCGT
MluNI 4392 TGGCCA TGG^CCA
MscI 4392 TGGCCA TGG^CCA
NarI 508 GGCGCC GG^CGCC
PinAI 121 ACCGGT A^CCGGT
PshAI 2588 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5864 AACGTT AA^CGTT
ScaI 4179 AGTACT AGT^ACT
SfoI 508 GGCGCC GGC^GCC
SspI 2287 AATATT AAT^ATT
StuI 1644 AGGCCT AGG^CCT
XbaI 5697 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 508 GGCGCC G[GA]^CG[CT]C
5832 GACGCC
AflII 1063 CTTAAG C^TTAAG
3827 CTTAAG
ApaI 1232 GGGCCC GGGCC^C
2730 GGGCCC
AseI 2219 ATTAAT AT^TAAT
5731 ATTAAT
AsnI 2219 ATTAAT AT^TAAT
5731 ATTAAT
AvaI 3226 CTCGGG C^[CT]CG[GA]G
4475 CTCGGG
BbsI 1949 GAAGAC GAAGAC(2/6)
5530 GAAGAC
BfrI 1063 CTTAAG C^TTAAG
3827 CTTAAG
BpuAI 1949 GAAGAC GAAGAC(2/6)
5530 GAAGAC
BsaHI 508 GGCGCC G[GA]^CG[CT]C
5832 GACGCC
BsmBI 4412 CGTCTC CGTCTC(1/5)
2068 GAGACG
BsmI 1741 GAATGC GAATGC(1/-1)
4943 GCATTC
BsoBI 3226 CTCGGG C^[CT]CG[GA]G
4475 CTCGGG
Bsp1407I 2232 TGTACA T^GTACA
4287 TGTACA
BspHI 1818 TCATGA T^CATGA
3636 TCATGA
BsrFI 121 ACCGGT [GA]^CCGG[CT]
5519 GCCGGT
BsrGI 2232 TGTACA T^GTACA
4287 TGTACA
Cfr10I 121 ACCGGT [GA]^CCGG[CT]
5519 GCCGGT
Hin1I 508 GGCGCC G[GA]^CG[CT]C
5832 GACGCC
NcoI 522 CCATGG C^CATGG
3431 CCATGG
PshBI 2219 ATTAAT AT^TAAT
5731 ATTAAT
RcaI 1818 TCATGA T^CATGA
3636 TCATGA
SacI 1369 GAGCTC GAGCT^C
5570 GAGCTC
SapI 5117 GAAGAGC GCTCTTC(1/4)
637 GCTCTTC
SspBI 2232 TGTACA T^GTACA
4287 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AspI AvaII BanI
BanII BbvCI BbvI BciVI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NdeI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI