##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI AscI AseI AsnI
Asp718I AspEI AvrII BbeI BfrI
BlnI BsaBI BsaHI BseAI BsiWI
BsmBI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoT22I
FseI HgaI Hin1I KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I RsrII SacI
SapI SbfI SfiI SfoI SfuI
SnaBI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 3745 CGCG CG^CG
AgeI 293 ACCGGT A^CCGGT
Alw44I 297 GTGCAC G^TGCAC
Aor51HI 4682 AGCGCT AGC^GCT
ApaI 2855 GGGCCC GGGCC^C
ApaLI 297 GTGCAC G^TGCAC
AviII 4918 TGCGCA TGC^GCA
BbrPI 3382 CACGTG CAC^GTG
BcgI 2315 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 691 GTATCC GTATCC(6/5)
BglI 392 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3902 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3902 GCTAAGC GC^T[ACGT]AGC
BsaWI 293 ACCGGT [AT]^CCGG[AT]
BsiEI 20 CGATCG CG[GA][CT]^CG
Bsp1407I 4924 TGTACA T^GTACA
BspLU11I 3517 ACATGT A^CATGT
BspMI 1350 ACCTGC ACCTGC(4/8)
BsrGI 4924 TGTACA T^GTACA
BstEII 4974 GGTCACC G^GT[ACGT]ACC
BstPI 4974 GGTCACC G^GT[ACGT]ACC
BstUI 3745 CGCG CG^CG
CelII 3902 GCTAAGC GC^T[ACGT]AGC
DraIII 251 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3974 GACAGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4682 AGCGCT AGC^GCT
EcoNI 1338 CCTGTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4974 GGTCACC G^GT[ACGT]ACC
EcoRI 4066 GAATTC G^AATTC
EcoRV 2078 GATATC GAT^ATC
FspI 4918 TGCGCA TGC^GCA
HindIII 3682 AAGCTT A^AGCTT
HpaI 529 GTTAAC GTT^AAC
KspI 3744 CCGCGG CCGC^GG
MvnI 3745 CGCG CG^CG
NdeI 1507 CATATG CA^TATG
PaeR7I 3458 CTCGAG C^TCGAG
PinAI 293 ACCGGT A^CCGGT
PmaCI 3382 CACGTG CAC^GTG
PmlI 3382 CACGTG CAC^GTG
PvuI 20 CGATCG CGAT^CG
SacII 3744 CCGCGG CCGC^GG
SalI 3104 GTCGAC G^TCGAC
ScaI 794 AGTACT AGT^ACT
SgrAI 292 CACCGGTG C[GA]^CCGG[CT]G
SpeI 555 ACTAGT A^CTAGT
SspBI 4924 TGTACA T^GTACA
XbaI 1563 TCTAGA T^CTAGA
XcmI 446 CCACTCTTCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3458 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2801 GTCTAC GT^[AC][GT]AC
3104 GTCGAC
AspI 1808 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5523 GACCCTGTC
BalI 2417 TGGCCA TGG^CCA
4599 TGGCCA
BclI 4803 TGATCA T^GATCA
5105 TGATCA
BglII 192 AGATCT A^GATCT
4949 AGATCT
BsmI 4372 GAATGC GAATGC(1/-1)
401 GCATTC
BspHI 1790 TCATGA T^CATGA
2848 TCATGA
BsrBI 3467 CCGCTC CCGCTC(-3/-3)
3212 GAGCGG
BstAPI 2290 GCAATCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3552 GCAATCGGTGC
FbaI 4803 TGATCA T^GATCA
5105 TGATCA
MluNI 2417 TGGCCA TGG^CCA
4599 TGGCCA
MscI 2417 TGGCCA TGG^CCA
4599 TGGCCA
PflFI 1808 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5523 GACCCTGTC
PflMI 4319 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5322 CCAGGAGTTGG
PpuMI 1812 AGGTCCT [GA]G^G[AT]CC[CT]
4507 AGGACCT
PstI 4120 CTGCAG CTGCA^G
5471 CTGCAG
RcaI 1790 TCATGA T^CATGA
2848 TCATGA
SexAI 2748 ACCAGGT A^CC[AT]GGT
3268 ACCAGGT
SmaI 4557 CCCGGG CCC^GGG
4848 CCCGGG
SspI 1454 AATATT AAT^ATT
5227 AATATT
Tth111I 1808 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5523 GACCCTGTC
Van91I 4319 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5322 CCAGGAGTTGG
XmaCI 4557 CCCGGG C^CCGGG
4848 CCCGGG
XmaI 4557 CCCGGG C^CCGGG
4848 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI