##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
ApaI AscI AseI AsnI Asp700I
Asp718I AviII BbrPI BciVI BclI
BfrI BsaBI BsiWI BspDI BspLU11I
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV EcoT22I FbaI FspI
HpaI KpnI MamI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SapI ScaI SexAI SfuI
SgrAI SpeI SphI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1961 GACGTC GACGT^C
AccIII 4288 TCCGGA T^CCGGA
AcsI 4833 GAATTC [GA]^AATT[CT]
AflIII 2587 ACACGT A^C[GA][CT]GT
AhdI 2191 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1064 GTGCAC G^TGCAC
AocI 104 CCTGAGG CC^T[ACGT]AGG
ApaLI 1064 GTGCAC G^TGCAC
ApoI 4833 GAATTC [GA]^AATT[CT]
AspEI 2191 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4659 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3820 CCTAGG C^CTAGG
BbeI 5059 GGCGCC GGCGC^C
BbsI 1188 GTCTTC GAAGAC(2/6)
BcgI 227 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3786 AGATCT A^GATCT
BlnI 3820 CCTAGG C^CTAGG
BpuAI 1188 GTCTTC GAAGAC(2/6)
BsaWI 4288 TCCGGA [AT]^CCGG[AT]
BseAI 4288 TCCGGA T^CCGGA
BsiEI 3799 CGGTCG CG[GA][CT]^CG
BspEI 4288 TCCGGA T^CCGGA
BspHI 3891 TCATGA T^CATGA
Bsu36I 104 CCTGAGG CC^T[ACGT]AGG
Eam1105I 2191 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 104 CCTGAGG CC^T[ACGT]AGG
EcoRI 4833 GAATTC G^AATTC
FseI 2034 GGCCGGCC GGCCGG^CC
HindIII 2536 AAGCTT A^AGCTT
KasI 5059 GGCGCC G^GCGCC
MroI 4288 TCCGGA T^CCGGA
NarI 5059 GGCGCC GG^CGCC
NcoI 2692 CCATGG C^CATGG
NheI 462 GCTAGC G^CTAGC
PaeR7I 2786 CTCGAG C^TCGAG
PflFI 4659 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1349 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3891 TCATGA T^CATGA
SfiI 3531 GGCCCTTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5059 GGCGCC GGC^GCC
SnaBI 4871 TACGTA TAC^GTA
SspI 1782 AATATT AAT^ATT
Tth111I 4659 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2786 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Aor51HI 2272 AGCGCT AGC^GCT
3959 AGCGCT
BamHI 369 GGATCC G^GATCC
1162 GGATCC
BbvCI 3097 CCTCAGC CCTCAGC(-5/-2)
1394 GCTGAGG
BlpI 1590 GCTCAGC GC^T[ACGT]AGC
5084 GCTGAGC
Bpu1102I 1590 GCTCAGC GC^T[ACGT]AGC
5084 GCTGAGC
BsmBI 639 GAGACG CGTCTC(1/5)
3933 GAGACG
BsmI 825 GAATGC GAATGC(1/-1)
4529 GCATTC
Bsp1407I 1237 TGTACA T^GTACA
1745 TGTACA
BsrBI 618 GAGCGG CCGCTC(-3/-3)
2611 GAGCGG
BsrFI 2035 GCCGGC [GA]^CCGG[CT]
2738 GCCGGC
BsrGI 1237 TGTACA T^GTACA
1745 TGTACA
CelII 1590 GCTCAGC GC^T[ACGT]AGC
5084 GCTGAGC
Cfr10I 2035 GCCGGC [GA]^CCGG[CT]
2738 GCCGGC
DraI 1619 TTTAAA TTT^AAA
3295 TTTAAA
Eco47III 2272 AGCGCT AGC^GCT
3959 AGCGCT
HincII 55 GTTGAC GT[CT]^[GA]AC
3900 GTCAAC
HindII 55 GTTGAC GT[CT]^[GA]AC
3900 GTCAAC
KspI 1221 CCGCGG CCGC^GG
4559 CCGCGG
NaeI 2035 GCCGGC GCC^GGC
2738 GCCGGC
NgoMIV 2035 GCCGGC G^CCGGC
2738 GCCGGC
NspI 754 ACATGC [GA]CATG^[CT]
1550 GCATGT
PpuMI 600 AGGTCCT [GA]G^G[AT]CC[CT]
1448 GGGTCCT
SacII 1221 CCGCGG CCGC^GG
4559 CCGCGG
SspBI 1237 TGTACA T^GTACA
1745 TGTACA
XbaI 553 TCTAGA T^CTAGA
3981 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmaI SmlI Sse8387I StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI