##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AflII AgeI Aor51HI
AscI Asp700I Asp718I AvrII BfrI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoNI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PvuI RsrII SacII SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 4822 GACGTC GACGT^C
AccI 1572 GTCTAC GT^[AC][GT]AC
AccII 2008 CGCG CG^CG
AclI 3921 AACGTT AA^CGTT
Alw44I 1008 GTGCAC G^TGCAC
ApaLI 1008 GTGCAC G^TGCAC
AspI 558 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4181 TGCGCA TGC^GCA
BamHI 5220 GGATCC G^GATCC
BbeI 4551 GGCGCC GGCGC^C
BbrPI 4629 CACGTG CAC^GTG
BcgI 4942 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3579 GTATCC GTATCC(6/5)
BglI 3714 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1949 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1949 GCTCAGC GC^T[ACGT]AGC
BsaWI 1488 TCCGGT [AT]^CCGG[AT]
Bsp1407I 5073 TGTACA T^GTACA
BsrBI 4105 CCGCTC CCGCTC(-3/-3)
BsrGI 5073 TGTACA T^GTACA
BssSI 76 CACGAG CACGAG(-5/-1)
BstEII 2205 GGTAACC G^GT[ACGT]ACC
BstPI 2205 GGTAACC G^GT[ACGT]ACC
BstUI 2008 CGCG CG^CG
CelII 1949 GCTCAGC GC^T[ACGT]AGC
DrdI 1587 GACTTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2205 GGTAACC G^GT[ACGT]ACC
EcoRI 1387 GAATTC G^AATTC
EcoRV 3401 GATATC GAT^ATC
EcoT22I 896 ATGCAT ATGCA^T
FspI 4181 TGCGCA TGC^GCA
KasI 4551 GGCGCC G^GCGCC
MvnI 2008 CGCG CG^CG
NarI 4551 GGCGCC GG^CGCC
NcoI 919 CCATGG C^CATGG
NsiI 896 ATGCAT ATGCA^T
PflFI 558 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2522 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4629 CACGTG CAC^GTG
PmlI 4629 CACGTG CAC^GTG
PshAI 867 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3921 AACGTT AA^CGTT
SapI 2541 GAAGAGC GCTCTTC(1/4)
SbfI 3170 CCTGCAGG CCTGCA^GG
SfoI 4551 GGCGCC GGC^GCC
Sse8387I 3170 CCTGCAGG CCTGCA^GG
SspBI 5073 TGTACA T^GTACA
SspI 1342 AATATT AAT^ATT
SwaI 5108 ATTTAAAT ATTT^AAAT
Tth111I 558 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2522 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 4551 GGCGCC G[GA]^CG[CT]C
4822 GACGTC
AflIII 3961 ACACGT A^C[GA][CT]GT
4628 ACACGT
AhdI 2629 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3957 GACAACACGTC
AspEI 2629 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3957 GACAACACGTC
AvaI 4127 CCCGGG C^[CT]CG[GA]G
4960 CCCGGG
BbsI 279 GTCTTC GAAGAC(2/6)
1674 GTCTTC
BpuAI 279 GTCTTC GAAGAC(2/6)
1674 GTCTTC
BsaAI 4629 CACGTG [CT]AC^GT[GA]
5149 TACGTG
BsaHI 4551 GGCGCC G[GA]^CG[CT]C
4822 GACGTC
BsmI 1980 GCATTC GAATGC(1/-1)
3496 GCATTC
BsoBI 4127 CCCGGG C^[CT]CG[GA]G
4960 CCCGGG
DraIII 1014 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2523 CACATGGTG
DsaI 919 CCATGG C^C[GA][CT]GG
1862 CCGTGG
Eam1105I 2629 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3957 GACAACACGTC
HaeII 4551 GGCGCC [GA]GCGC^[CT]
4702 GGCGCT
Hin1I 4551 GGCGCC G[GA]^CG[CT]C
4822 GACGTC
PpuMI 1855 GGGACCT [GA]G^G[AT]CC[CT]
2337 GGGACCC
SexAI 125 ACCAGGT A^CC[AT]GGT
5183 ACCAGGT
SmaI 4127 CCCGGG CCC^GGG
4960 CCCGGG
SphI 675 GCATGC GCATG^C
3535 GCATGC
XcmI 1087 CCAGGGCCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3371 CCAACTGTCTAATGG
XmaCI 4127 CCCGGG C^CCGGG
4960 CCCGGG
XmaI 4127 CCCGGG C^CCGGG
4960 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AseI
AsnI AspHI AvaII BalI BanI
BanII BbvCI BbvI BclI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII