##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII BalI BamHI BcgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BsrFI BsrGI BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRV EcoT22I
FseI FspI HgaI HindIII HpaI
KpnI KspI MfeI MluI MluNI
MscI MunI NaeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 3246 TCCGGA T^CCGGA
AcyI 897 GGCGCC G[GA]^CG[CT]C
Alw44I 2158 GTGCAC G^TGCAC
ApaLI 2158 GTGCAC G^TGCAC
AseI 4727 ATTAAT AT^TAAT
AsnI 4727 ATTAAT AT^TAAT
Asp700I 2834 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3955 CCCGGG C^[CT]CG[GA]G
BbeI 897 GGCGCC GGCGC^C
BglI 311 GCCTATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 5502 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5502 GCTGAGC GC^T[ACGT]AGC
BsaBI 3765 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 897 GGCGCC G[GA]^CG[CT]C
BsaWI 3246 TCCGGA [AT]^CCGG[AT]
BseAI 3246 TCCGGA T^CCGGA
BsoBI 3955 CCCGGG C^[CT]CG[GA]G
BspEI 3246 TCCGGA T^CCGGA
BspLU11I 2347 ACATGT A^CATGT
BssHII 599 GCGCGC G^CGCGC
BstAPI 73 GCATGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3666 GGTGACC G^GT[ACGT]ACC
BstPI 3666 GGTGACC G^GT[ACGT]ACC
CelII 5502 GCTGAGC GC^T[ACGT]AGC
DrdI 3087 GACGTGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2292 CCATGG C^C[GA][CT]GG
EcoO65I 3666 GGTGACC G^GT[ACGT]ACC
Hin1I 897 GGCGCC G[GA]^CG[CT]C
KasI 897 GGCGCC G^GCGCC
MamI 3765 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3246 TCCGGA T^CCGGA
NarI 897 GGCGCC GG^CGCC
NcoI 2292 CCATGG C^CATGG
NheI 3432 GCTAGC G^CTAGC
PshAI 1570 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4727 ATTAAT AT^TAAT
ScaI 655 AGTACT AGT^ACT
SfoI 897 GGCGCC GGC^GCC
SmaI 3955 CCCGGG CCC^GGG
XmaCI 3955 CCCGGG C^CCGGG
XmaI 3955 CCCGGG C^CCGGG
XmnI 2834 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 1962 CTTAAG C^TTAAG
4868 CTTAAG
AvrII 785 CCTAGG C^CTAGG
929 CCTAGG
BbrPI 564 CACGTG CAC^GTG
2072 CACGTG
BclI 1319 TGATCA T^GATCA
3716 TGATCA
BfrI 1962 CTTAAG C^TTAAG
4868 CTTAAG
BlnI 785 CCTAGG C^CTAGG
929 CCTAGG
BsaAI 564 CACGTG [CT]AC^GT[GA]
2072 CACGTG
BsgI 2364 CTGCAC GTGCAG(16/14)
4584 GTGCAG
BsmI 5222 GAATGC GAATGC(1/-1)
2165 GCATTC
BspMI 771 ACCTGC ACCTGC(4/8)
1619 GCAGGT
BsrBI 5338 CCGCTC CCGCTC(-3/-3)
1233 GAGCGG
DraI 2012 TTTAAA TTT^AAA
4504 TTTAAA
EcoRI 3807 GAATTC G^AATTC
4932 GAATTC
FbaI 1319 TGATCA T^GATCA
3716 TGATCA
NdeI 2339 CATATG CA^TATG
3228 CATATG
PmaCI 564 CACGTG CAC^GTG
2072 CACGTG
PmlI 564 CACGTG CAC^GTG
2072 CACGTG
PstI 773 CTGCAG CTGCA^G
2712 CTGCAG
SacI 498 GAGCTC GAGCT^C
1841 GAGCTC
SapI 834 GAAGAGC GCTCTTC(1/4)
41 GCTCTTC
SexAI 694 ACCTGGT A^CC[AT]GGT
793 ACCTGGT
SmlI 1962 CTTAAG C^T[CT][GA]AG
4868 CTTAAG
XbaI 4442 TCTAGA T^CTAGA
5066 TCTAGA
XcmI 493 CCAAAGAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3529 CCAGATATGGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AspI AvaII BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII