##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
AspI AviII AvrII BbeI BbrPI
BcgI BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiWI BspEI BsrBI
BsrDI BssHII Bst1107I BstBI BstZ17I
CelII CpoI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FseI FspI
KasI KspI MluI MroI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 982 CGCG CG^CG
AcsI 721 GAATTT [GA]^AATT[CT]
AcyI 2998 GGCGTC G[GA]^CG[CT]C
AflII 148 CTTAAG C^TTAAG
AhdI 4022 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3008 AGCGCT AGC^GCT
ApoI 721 GAATTT [GA]^AATT[CT]
AspEI 4022 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3054 TGGCCA TGG^CCA
BamHI 738 GGATCC G^GATCC
BciVI 369 GTATCC GTATCC(6/5)
BfrI 148 CTTAAG C^TTAAG
BglII 1554 AGATCT A^GATCT
BsaBI 4707 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2998 GGCGTC G[GA]^CG[CT]C
BsaI 1324 GGTCTC GGTCTC(1/5)
BsaWI 3154 TCCGGT [AT]^CCGG[AT]
BsmBI 3402 CGTCTC CGTCTC(1/5)
BsmI 4644 GAATGC GAATGC(1/-1)
Bsp1407I 1529 TGTACA T^GTACA
BspDI 2321 ATCGAT AT^CGAT
BspHI 1136 TCATGA T^CATGA
BspLU11I 2258 ACATGT A^CATGT
BsrFI 977 ACCGGC [GA]^CCGG[CT]
BsrGI 1529 TGTACA T^GTACA
BssSI 2231 CTCGTG CACGAG(-5/-1)
BstEII 4019 GGTGACC G^GT[ACGT]ACC
BstPI 4019 GGTGACC G^GT[ACGT]ACC
BstUI 982 CGCG CG^CG
Cfr10I 977 ACCGGC [GA]^CCGG[CT]
ClaI 2321 ATCGAT AT^CGAT
DraI 4856 TTTAAA TTT^AAA
Eam1105I 4022 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3008 AGCGCT AGC^GCT
EcoO65I 4019 GGTGACC G^GT[ACGT]ACC
HgaI 2999 GCGTC GACGC(5/10)
Hin1I 2998 GGCGTC G[GA]^CG[CT]C
HpaI 2953 GTTAAC GTT^AAC
MamI 4707 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4285 CAATTG C^AATTG
MluNI 3054 TGGCCA TGG^CCA
MscI 3054 TGGCCA TGG^CCA
MunI 4285 CAATTG C^AATTG
MvnI 982 CGCG CG^CG
PflMI 923 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1136 TCATGA T^CATGA
SexAI 3242 ACCAGGT A^CC[AT]GGT
SgrAI 976 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1529 TGTACA T^GTACA
StuI 2653 AGGCCT AGG^CCT
TaqI 2322 TCGA T^CGA
TthHB8I 2322 TCGA T^CGA
Van91I 923 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1725 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 1046 GGTACC G^GTACC
3824 GGTACC
AflIII 240 ACACGT A^C[GA][CT]GT
2258 ACATGT
ApaI 918 GGGCCC GGGCC^C
3104 GGGCCC
Asp718I 1046 GGTACC G^GTACC
3824 GGTACC
AspHI 1018 GAGCAC G[AT]GC[AT]^C
1878 GTGCTC
AvaI 2907 CTCGGG C^[CT]CG[GA]G
3705 CCCGAG
BbsI 4199 GAAGAC GAAGAC(2/6)
2865 GTCTTC
BbvCI 102 CCTCAGC CCTCAGC(-5/-2)
969 GCTGAGG
BclI 3846 TGATCA T^GATCA
4367 TGATCA
BpuAI 4199 GAAGAC GAAGAC(2/6)
2865 GTCTTC
BsiHKAI 1018 GAGCAC G[AT]GC[AT]^C
1878 GTGCTC
BsoBI 2907 CTCGGG C^[CT]CG[GA]G
3705 CCCGAG
BstAPI 1020 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1390 GCATGGGTTGC
BstXI 3761 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3791 CCAAGAATGTGG
DraIII 2968 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4160 CACAAGGTG
DrdI 799 GACTTGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3289 GACATCGTGGTC
FbaI 3846 TGATCA T^GATCA
4367 TGATCA
HaeII 1000 AGCGCC [GA]GCGC^[CT]
3008 AGCGCT
KpnI 1046 GGTACC GGTAC^C
3824 GGTACC
SapI 3729 GCTCTTC GCTCTTC(1/4)
4588 GCTCTTC
SmlI 148 CTTAAG C^T[CT][GA]AG
1953 CTCAAG
XcmI 70 CCAGGCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3675 CCATCCCCTCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Asp700I AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI