##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AflIII AgeI AhdI AscI
Asp700I AspEI BamHI BbrPI BcgI
BciVI BsaAI BsaBI Bsp1407I BspDI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eam1105I
EcoRV EcoT22I FseI HpaI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SapI SfuI SnaBI SpeI
SspBI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 547 GACGTC GACGT^C
AccI 3500 GTCGAC GT^[AC][GT]AC
AccIII 809 TCCGGA T^CCGGA
AcsI 519 GAATTC [GA]^AATT[CT]
AflII 304 CTTAAG C^TTAAG
ApoI 519 GAATTC [GA]^AATT[CT]
AseI 5065 ATTAAT AT^TAAT
AsnI 5065 ATTAAT AT^TAAT
AviII 2112 TGCGCA TGC^GCA
AvrII 2591 CCTAGG C^CTAGG
BclI 4273 TGATCA T^GATCA
BfrI 304 CTTAAG C^TTAAG
BlnI 2591 CCTAGG C^CTAGG
BsaWI 809 TCCGGA [AT]^CCGG[AT]
BseAI 809 TCCGGA T^CCGGA
BsiWI 2950 CGTACG C^GTACG
BsmBI 136 GAGACG CGTCTC(1/5)
BspEI 809 TCCGGA T^CCGGA
BssHII 1680 GCGCGC G^CGCGC
BstAPI 3515 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4714 GGTCACC G^GT[ACGT]ACC
BstPI 4714 GGTCACC G^GT[ACGT]ACC
DrdI 547 GACGTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 701 CGGCCG C^GGCCG
EclXI 701 CGGCCG C^GGCCG
Eco52I 701 CGGCCG C^GGCCG
EcoO65I 4714 GGTCACC G^GT[ACGT]ACC
EcoRI 519 GAATTC G^AATTC
FbaI 4273 TGATCA T^GATCA
FspI 2112 TGCGCA TGC^GCA
HindIII 4558 AAGCTT A^AGCTT
MroI 809 TCCGGA T^CCGGA
NaeI 2342 GCCGGC GCC^GGC
NgoMIV 2342 GCCGGC G^CCGGC
PshBI 5065 ATTAAT AT^TAAT
SacI 2721 GAGCTC GAGCT^C
SalI 3500 GTCGAC G^TCGAC
SexAI 3532 ACCTGGT A^CC[AT]GGT
SfiI 2870 GGCCAACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1547 CACCGGCG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 4529 GTGCAC G^TGCAC
5002 GTGCAC
Aor51HI 1859 AGCGCT AGC^GCT
2814 AGCGCT
ApaLI 4529 GTGCAC G^TGCAC
5002 GTGCAC
BbeI 1733 GGCGCC GGCGC^C
1885 GGCGCC
BglII 2321 AGATCT A^GATCT
4633 AGATCT
BsmI 2659 GAATGC GAATGC(1/-1)
3341 GAATGC
BspHI 581 TCATGA T^CATGA
611 TCATGA
DraIII 1584 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4266 CACTCAGTG
EarI 199 CTCTTC CTCTTC(1/4)
4347 GAAGAG
Eco47III 1859 AGCGCT AGC^GCT
2814 AGCGCT
HincII 3500 GTCGAC GT[CT]^[GA]AC
3668 GTTGAC
HindII 3500 GTCGAC GT[CT]^[GA]AC
3668 GTTGAC
KasI 1733 GGCGCC G^GCGCC
1885 GGCGCC
Ksp632I 199 CTCTTC CTCTTC(1/4)
4347 GAAGAG
MslI 205 CACCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3179 CATGCCGGTG
NarI 1733 GGCGCC GG^CGCC
1885 GGCGCC
RcaI 581 TCATGA T^CATGA
611 TCATGA
SbfI 265 CCTGCAGG CCTGCA^GG
2957 CCTGCAGG
ScaI 3273 AGTACT AGT^ACT
3802 AGTACT
SfoI 1733 GGCGCC GGC^GCC
1885 GGCGCC
Sse8387I 265 CCTGCAGG CCTGCA^GG
2957 CCTGCAGG
SspI 803 AATATT AAT^ATT
1009 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI