##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BbeI BciVI BglI BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BsrDI
BssHII BstBI BstEII BstPI ClaI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI FspI
HpaI KasI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NspV PacI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RcaI SacII SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 2723 GGTACC G^GTACC
AcsI 3057 AAATTC [GA]^AATT[CT]
AcyI 637 GGCGTC G[GA]^CG[CT]C
AflII 2497 CTTAAG C^TTAAG
AhdI 1694 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3057 AAATTC [GA]^AATT[CT]
Asp718I 2723 GGTACC G^GTACC
AspEI 1694 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2637 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1283 TGGCCA TGG^CCA
BamHI 2492 GGATCC G^GATCC
BbrPI 1407 CACGTG CAC^GTG
BbvCI 2069 CCTCAGC CCTCAGC(-5/-2)
BcgI 965 CGAGTCATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2534 TGATCA T^GATCA
BfrI 2497 CTTAAG C^TTAAG
BglII 1082 AGATCT A^GATCT
BsaAI 1407 CACGTG [CT]AC^GT[GA]
BsaHI 637 GGCGTC G[GA]^CG[CT]C
BsmI 241 GAATGC GAATGC(1/-1)
BssSI 1764 CACGAG CACGAG(-5/-1)
Bst1107I 3084 GTATAC GTA^TAC
BstZ17I 3084 GTATAC GTA^TAC
CpoI 1337 CGGACCG CG^G[AT]CCG
DraIII 31 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1694 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1926 ATGCAT ATGCA^T
FbaI 2534 TGATCA T^GATCA
Hin1I 637 GGCGTC G[GA]^CG[CT]C
HincII 1332 GTCGAC GT[CT]^[GA]AC
HindII 1332 GTCGAC GT[CT]^[GA]AC
HindIII 1949 AAGCTT A^AGCTT
KpnI 2723 GGTACC GGTAC^C
MluNI 1283 TGGCCA TGG^CCA
MscI 1283 TGGCCA TGG^CCA
NheI 2209 GCTAGC G^CTAGC
NsiI 1926 ATGCAT ATGCA^T
PaeR7I 154 CTCGAG C^TCGAG
PflFI 2637 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1407 CACGTG CAC^GTG
PmlI 1407 CACGTG CAC^GTG
PvuII 1435 CAGCTG CAG^CTG
RsrII 1337 CGGACCG CG^G[AT]CCG
SalI 1332 GTCGAC G^TCGAC
SapI 1188 GCTCTTC GCTCTTC(1/4)
SbfI 1426 CCTGCAGG CCTGCA^GG
ScaI 1288 AGTACT AGT^ACT
SmaI 1371 CCCGGG CCC^GGG
Sse8387I 1426 CCTGCAGG CCTGCA^GG
SspI 3189 AATATT AAT^ATT
Tth111I 2637 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 803 CCATGAAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 154 CTCGAG C^TCGAG
XmaCI 1371 CCCGGG C^CCGGG
XmaI 1371 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1332 GTCGAC GT^[AC][GT]AC
3084 GTATAC
AccII 1072 CGCG CG^CG
1592 CGCG
AflIII 1517 ACATGT A^C[GA][CT]GT
3080 ACATGT
ApaI 132 GGGCCC GGGCC^C
1559 GGGCCC
BlpI 2197 GCTCAGC GC^T[ACGT]AGC
2668 GCTGAGC
Bpu1102I 2197 GCTCAGC GC^T[ACGT]AGC
2668 GCTGAGC
BspLU11I 1517 ACATGT A^CATGT
3080 ACATGT
BspMI 524 ACCTGC ACCTGC(4/8)
542 ACCTGC
BstUI 1072 CGCG CG^CG
1592 CGCG
BstXI 1616 CCAGAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2769 CCAGTGCAGTGG
BstYI 1082 AGATCT [GA]^GATC[CT]
2492 GGATCC
CelII 2197 GCTCAGC GC^T[ACGT]AGC
2668 GCTGAGC
DraI 1799 TTTAAA TTT^AAA
3148 TTTAAA
EcoNI 1305 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2868 CCTCTGTGAGG
HaeII 110 AGCGCC [GA]GCGC^[CT]
772 AGCGCC
MflI 1082 AGATCT [GA]^GATC[CT]
2492 GGATCC
MvnI 1072 CGCG CG^CG
1592 CGCG
NaeI 550 GCCGGC GCC^GGC
614 GCCGGC
NcoI 892 CCATGG C^CATGG
2021 CCATGG
NgoMIV 550 GCCGGC G^CCGGC
614 GCCGGC
PpuMI 984 GGGACCT [GA]G^G[AT]CC[CT]
1197 GGGTCCT
PshAI 1462 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1714 GACCGCAGTC
StuI 2355 AGGCCT AGG^CCT
2517 AGGCCT
TfiI 264 GATTC G^A[AT]TC
3106 GATTC
Tsp509I 3058 AATT ^AATT
3174 AATT
XhoII 1082 AGATCT [GA]^GATC[CT]
2492 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI AocI ApaLI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I XmnI