##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BciVI
BfrI BglI BlnI BsaBI BseAI
BsiWI BspDI BspEI BspLU11I BsrFI
BstBI Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco52I FseI FspI
KspI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 195 GGTACC G^GTACC
AccI 4099 GTATAC GT^[AC][GT]AC
AcyI 2975 GGCGCC G[GA]^CG[CT]C
AflIII 4904 ACGTGT A^C[GA][CT]GT
Aor51HI 673 AGCGCT AGC^GCT
Asp700I 692 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 195 GGTACC G^GTACC
BamHI 3304 GGATCC G^GATCC
BbeI 2975 GGCGCC GGCGC^C
BbrPI 3790 CACGTG CAC^GTG
BclI 3774 TGATCA T^GATCA
BsaAI 3790 CACGTG [CT]AC^GT[GA]
BsaHI 2975 GGCGCC G[GA]^CG[CT]C
BsaWI 2883 TCCGGT [AT]^CCGG[AT]
BsiEI 5356 CGACCG CG[GA][CT]^CG
BsmBI 4792 CGTCTC CGTCTC(1/5)
BsmI 2745 GAATGC GAATGC(1/-1)
BspHI 233 TCATGA T^CATGA
BsrDI 5152 GCAATG GCAATG(2/0)
BssHII 2936 GCGCGC G^CGCGC
Bst1107I 4099 GTATAC GTA^TAC
BstEII 3703 GGTGACC G^GT[ACGT]ACC
BstPI 3703 GGTGACC G^GT[ACGT]ACC
BstZ17I 4099 GTATAC GTA^TAC
Eco47III 673 AGCGCT AGC^GCT
EcoNI 973 CCTAACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3703 GGTGACC G^GT[ACGT]ACC
EcoRI 5004 GAATTC G^AATTC
EcoRV 4280 GATATC GAT^ATC
FbaI 3774 TGATCA T^GATCA
Hin1I 2975 GGCGCC G[GA]^CG[CT]C
HpaI 5320 GTTAAC GTT^AAC
KasI 2975 GGCGCC G^GCGCC
KpnI 195 GGTACC GGTAC^C
NarI 2975 GGCGCC GG^CGCC
PflMI 5463 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3790 CACGTG CAC^GTG
PmlI 3790 CACGTG CAC^GTG
RcaI 233 TCATGA T^CATGA
SapI 695 GCTCTTC GCTCTTC(1/4)
ScaI 4248 AGTACT AGT^ACT
SexAI 5568 ACCAGGT A^CC[AT]GGT
SfoI 2975 GGCGCC GGC^GCC
Van91I 5463 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2450 TCTAGA T^CTAGA
XmnI 692 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaI 476 CCCGGG C^[CT]CG[GA]G
854 CCCGGG
BcgI 5565 CGAACCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4974 GCATGCAGCTCG
BglII 745 AGATCT A^GATCT
2077 AGATCT
BsoBI 476 CCCGGG C^[CT]CG[GA]G
854 CCCGGG
Bsp1407I 3425 TGTACA T^GTACA
4236 TGTACA
BsrGI 3425 TGTACA T^GTACA
4236 TGTACA
BstXI 1755 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4546 CCAGCACTTTGG
DraIII 2999 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
3055 CACGGTGTG
DrdI 3071 GACAGGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4854 GACAAGCTTGTC
EcoT22I 3517 ATGCAT ATGCA^T
5415 ATGCAT
NcoI 2768 CCATGG C^CATGG
5633 CCATGG
NsiI 3517 ATGCAT ATGCA^T
5415 ATGCAT
SacI 616 GAGCTC GAGCT^C
3890 GAGCTC
SmaI 476 CCCGGG CCC^GGG
854 CCCGGG
SphI 4974 GCATGC GCATG^C
5417 GCATGC
SspBI 3425 TGTACA T^GTACA
4236 TGTACA
XmaCI 476 CCCGGG C^CCGGG
854 CCCGGG
XmaI 476 CCCGGG C^CCGGG
854 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII