##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspI AviII BbeI BbvCI
BglII BlpI Bpu1102I BsaBI BseAI
BspDI BspEI BssHII Bst1107I BstBI
BstUI BstZ17I CelII CfoI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PflFI PmeI PshAI Psp1406I RsrII
SacII SapI SbfI SfiI SfoI
SfuI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 3476 CTTAAG C^TTAAG
AgeI 757 ACCGGT A^CCGGT
AocI 3211 CCTGAGG CC^T[ACGT]AGG
Asp700I 4902 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3274 CTCGAG C^[CT]CG[GA]G
AvrII 3070 CCTAGG C^CTAGG
BanI 3291 GGTGCC G^G[CT][GA]CC
BbrPI 5304 CACGTG CAC^GTG
BcgI 157 GCAAATTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3684 TGATCA T^GATCA
BcnI 3152 CCCGG CC^[GC]GG
BfrI 3476 CTTAAG C^TTAAG
BglI 880 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3070 CCTAGG C^CTAGG
BsaAI 5304 CACGTG [CT]AC^GT[GA]
BsaWI 757 ACCGGT [AT]^CCGG[AT]
BsgI 761 GTGCAG GTGCAG(16/14)
BsiEI 2872 CGATCG CG[GA][CT]^CG
BsiWI 2372 CGTACG C^GTACG
BsoBI 3274 CTCGAG C^[CT]CG[GA]G
BspHI 4815 TCATGA T^CATGA
BsrDI 3491 CATTGC GCAATG(2/0)
BsrFI 757 ACCGGT [GA]^CCGG[CT]
BssSI 5184 CACGAG CACGAG(-5/-1)
BstAPI 1129 GCATTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3211 CCTGAGG CC^T[ACGT]AGG
Cfr10I 757 ACCGGT [GA]^CCGG[CT]
DraIII 4285 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3211 CCTGAGG CC^T[ACGT]AGG
FbaI 3684 TGATCA T^GATCA
MfeI 1278 CAATTG C^AATTG
MunI 1278 CAATTG C^AATTG
NciI 3152 CCCGG CC^[GC]GG
PaeR7I 3274 CTCGAG C^TCGAG
PinAI 757 ACCGGT A^CCGGT
PmaCI 5304 CACGTG CAC^GTG
PmlI 5304 CACGTG CAC^GTG
PstI 3505 CTGCAG CTGCA^G
PvuI 2872 CGATCG CGAT^CG
RcaI 4815 TCATGA T^CATGA
SacI 1671 GAGCTC GAGCT^C
SalI 1702 GTCGAC G^TCGAC
SexAI 1884 ACCAGGT A^CC[AT]GGT
SgrAI 756 CACCGGTG C[GA]^CCGG[CT]G
SphI 4513 GCATGC GCATG^C
XhoI 3274 CTCGAG C^TCGAG
XmnI 4902 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1702 GTCGAC GT^[AC][GT]AC
2487 GTCTAC
AcyI 1496 GGCGTC G[GA]^CG[CT]C
1705 GACGCC
AflIII 2570 ACATGT A^C[GA][CT]GT
4810 ACATGT
AhdI 3413 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5104 GACTTCTTGTC
AspEI 3413 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5104 GACTTCTTGTC
BamHI 2247 GGATCC G^GATCC
4689 GGATCC
BanII 563 GGGCTC G[GA]GC[CT]^C
1671 GAGCTC
BciVI 72 GGATAC GTATCC(6/5)
4712 GGATAC
BsaHI 1496 GGCGTC G[GA]^CG[CT]C
1705 GACGCC
BsaI 3279 GAGACC GGTCTC(1/5)
3331 GAGACC
BsmI 2524 GCATTC GAATGC(1/-1)
5237 GCATTC
Bsp1407I 5214 TGTACA T^GTACA
5463 TGTACA
BspLU11I 2570 ACATGT A^CATGT
4810 ACATGT
BsrBI 3141 CCGCTC CCGCTC(-3/-3)
2641 GAGCGG
BsrGI 5214 TGTACA T^GTACA
5463 TGTACA
BstEII 3216 GGTTACC G^GT[ACGT]ACC
4031 GGTCACC
BstPI 3216 GGTTACC G^GT[ACGT]ACC
4031 GGTCACC
Eam1105I 3413 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5104 GACTTCTTGTC
EcoO65I 3216 GGTTACC G^GT[ACGT]ACC
4031 GGTCACC
EcoRI 2297 GAATTC G^AATTC
4532 GAATTC
HapII 758 CCGG C^CGG
3153 CCGG
Hin1I 1496 GGCGTC G[GA]^CG[CT]C
1705 GACGCC
HpaII 758 CCGG C^CGG
3153 CCGG
MspI 758 CCGG C^CGG
3153 CCGG
NheI 1366 GCTAGC G^CTAGC
2237 GCTAGC
PacI 18 TTAATTAA TTAAT^TAA
50 TTAATTAA
PflMI 1539 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4259 CCAGAAGCTGG
PvuII 1049 CAGCTG CAG^CTG
2357 CAGCTG
ScaI 750 AGTACT AGT^ACT
5090 AGTACT
SspBI 5214 TGTACA T^GTACA
5463 TGTACA
StuI 2504 AGGCCT AGG^CCT
3316 AGGCCT
Van91I 1539 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4259 CCAGAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BalI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HgaI HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII