##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI Aor51HI ApaI AscI AviII
BamHI BbeI BclI BglI BglII
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DrdI EagI
EarI EclXI Eco47III Eco52I EcoRV
FbaI FseI FspI Hin1I HindIII
HpaI KasI Ksp632I KspI MluI
MroI MspA1I NaeI NarI NgoMIV
NheI NotI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
PvuII RsrII SacII SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 87 GGTACC G^GTACC
AhdI 5353 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3367 ATTAAT AT^TAAT
AsnI 3367 ATTAAT AT^TAAT
Asp700I 795 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 87 GGTACC G^GTACC
AspEI 5353 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2535 CACGTG CAC^GTG
BsaAI 2535 CACGTG [CT]AC^GT[GA]
BsaBI 5266 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1461 TCATGA T^CATGA
BsrFI 2687 GCCGGT [GA]^CCGG[CT]
BstEII 1111 GGTAACC G^GT[ACGT]ACC
BstPI 1111 GGTAACC G^GT[ACGT]ACC
BstYI 1360 AGATCC [GA]^GATC[CT]
Cfr10I 2687 GCCGGT [GA]^CCGG[CT]
Eam1105I 5353 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 346 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1111 GGTAACC G^GT[ACGT]ACC
EcoT22I 700 ATGCAT ATGCA^T
HaeII 4784 AGCGCC [GA]GCGC^[CT]
HgaI 3828 GCGTC GACGC(5/10)
HincII 4326 GTCGAC GT[CT]^[GA]AC
HindII 4326 GTCGAC GT[CT]^[GA]AC
KpnI 87 GGTACC GGTAC^C
MamI 5266 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1410 CAATTG C^AATTG
MflI 1360 AGATCC [GA]^GATC[CT]
MunI 1410 CAATTG C^AATTG
NcoI 4130 CCATGG C^CATGG
NruI 3235 TCGCGA TCG^CGA
NsiI 700 ATGCAT ATGCA^T
PflMI 2361 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2535 CACGTG CAC^GTG
PmlI 2535 CACGTG CAC^GTG
PshBI 3367 ATTAAT AT^TAAT
PstI 4333 CTGCAG CTGCA^G
RcaI 1461 TCATGA T^CATGA
SalI 4326 GTCGAC G^TCGAC
SmaI 4732 CCCGGG CCC^GGG
SspI 3450 AATATT AAT^ATT
Van91I 2361 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4184 TCTAGA T^CTAGA
XhoII 1360 AGATCC [GA]^GATC[CT]
XmaCI 4732 CCCGGG C^CCGGG
XmaI 4732 CCCGGG C^CCGGG
XmnI 795 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 3288 GTCTAC GT^[AC][GT]AC
4326 GTCGAC
AflII 1959 CTTAAG C^TTAAG
2868 CTTAAG
AocI 2189 CCTCAGG CC^T[ACGT]AGG
4491 CCTGAGG
AvrII 346 CCTAGG C^CTAGG
3267 CCTAGG
BcgI 3256 CGATCCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4318 GCATGAATGTCG
BciVI 3976 GGATAC GTATCC(6/5)
5338 GGATAC
BfrI 1959 CTTAAG C^TTAAG
2868 CTTAAG
BlnI 346 CCTAGG C^CTAGG
3267 CCTAGG
Bsp1407I 318 TGTACA T^GTACA
2836 TGTACA
BspMI 3707 ACCTGC ACCTGC(4/8)
352 GCAGGT
BsrGI 318 TGTACA T^GTACA
2836 TGTACA
BssSI 5039 CTCGTG CACGAG(-5/-1)
5172 CTCGTG
Bsu36I 2189 CCTCAGG CC^T[ACGT]AGG
4491 CCTGAGG
DraI 3336 TTTAAA TTT^AAA
3376 TTTAAA
DraIII 1531 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4647 CACTTTGTG
DsaI 3075 CCGTGG C^C[GA][CT]GG
4130 CCATGG
Eco81I 2189 CCTCAGG CC^T[ACGT]AGG
4491 CCTGAGG
EcoRI 1721 GAATTC G^AATTC
5554 GAATTC
NdeI 1490 CATATG CA^TATG
3966 CATATG
SexAI 4995 ACCAGGT A^CC[AT]GGT
5704 ACCTGGT
SspBI 318 TGTACA T^GTACA
2836 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I