##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII BbeI BcgI
BciVI BsiEI BsiWI BspDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoT22I FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 5644 GTAGAC GT^[AC][GT]AC
AccIII 3925 TCCGGA T^CCGGA
AcyI 4292 GGCGTC G[GA]^CG[CT]C
AflIII 303 ACATGT A^C[GA][CT]GT
Alw44I 4534 GTGCAC G^TGCAC
AocI 2761 CCTGAGG CC^T[ACGT]AGG
ApaLI 4534 GTGCAC G^TGCAC
AvrII 4866 CCTAGG C^CTAGG
BbrPI 4037 CACGTG CAC^GTG
BbsI 779 GAAGAC GAAGAC(2/6)
BglI 4256 GCCCCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 752 AGATCT A^GATCT
BlnI 4866 CCTAGG C^CTAGG
BpuAI 779 GAAGAC GAAGAC(2/6)
BsaAI 4037 CACGTG [CT]AC^GT[GA]
BsaHI 4292 GGCGTC G[GA]^CG[CT]C
BseAI 3925 TCCGGA T^CCGGA
BspEI 3925 TCCGGA T^CCGGA
BspHI 671 TCATGA T^CATGA
BspLU11I 303 ACATGT A^CATGT
BsrBI 4299 CCGCTC CCGCTC(-3/-3)
BsrFI 5027 GCCGGT [GA]^CCGG[CT]
Bsu36I 2761 CCTGAGG CC^T[ACGT]AGG
Cfr10I 5027 GCCGGT [GA]^CCGG[CT]
DraIII 2393 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2761 CCTGAGG CC^T[ACGT]AGG
EcoNI 3506 CCTAACTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3360 GAATTC G^AATTC
Hin1I 4292 GGCGTC G[GA]^CG[CT]C
MroI 3925 TCCGGA T^CCGGA
NcoI 5560 CCATGG C^CATGG
NdeI 1971 CATATG CA^TATG
PaeR7I 5203 CTCGAG C^TCGAG
PmaCI 4037 CACGTG CAC^GTG
PmlI 4037 CACGTG CAC^GTG
PshAI 1754 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 671 TCATGA T^CATGA
SacI 4871 GAGCTC GAGCT^C
SapI 4836 GAAGAGC GCTCTTC(1/4)
SexAI 4275 ACCTGGT A^CC[AT]GGT
SphI 5062 GCATGC GCATG^C
XcmI 5131 CCAGCTCACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5203 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Asp700I 3862 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5549 GAAAAACTTC
BalI 2525 TGGCCA TGG^CCA
5082 TGGCCA
BamHI 4325 GGATCC G^GATCC
5154 GGATCC
BlpI 3620 GCTGAGC GC^T[ACGT]AGC
4483 GCTCAGC
Bpu1102I 3620 GCTGAGC GC^T[ACGT]AGC
4483 GCTCAGC
BsaBI 1473 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2314 GATCAGTATC
BsaI 2778 GAGACC GGTCTC(1/5)
4626 GGTCTC
BsaWI 943 TCCGGT [AT]^CCGG[AT]
3925 TCCGGA
BsgI 641 GTGCAG GTGCAG(16/14)
5076 GTGCAG
BsmBI 4294 CGTCTC CGTCTC(1/5)
4554 CGTCTC
Bsp1407I 2533 TGTACA T^GTACA
3718 TGTACA
BsrGI 2533 TGTACA T^GTACA
3718 TGTACA
BstXI 2516 CCACAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2728 CCAGCACTTTGG
CelII 3620 GCTGAGC GC^T[ACGT]AGC
4483 GCTCAGC
DsaI 2503 CCACGG C^C[GA][CT]GG
5560 CCATGG
EaeI 2525 TGGCCA [CT]^GGCC[GA]
5082 TGGCCA
EcoRV 346 GATATC GAT^ATC
605 GATATC
HgaI 4293 GCGTC GACGC(5/10)
4553 GCGTC
MamI 1473 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2314 GATCAGTATC
MluNI 2525 TGGCCA TGG^CCA
5082 TGGCCA
MscI 2525 TGGCCA TGG^CCA
5082 TGGCCA
MslI 1124 CATTAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5164 CACTAGCATG
PflMI 4283 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4428 CCAGACTTTGG
PpuMI 1759 AGGTCCC [GA]G^G[AT]CC[CT]
4730 GGGACCT
SpeI 161 ACTAGT A^CTAGT
2413 ACTAGT
SspBI 2533 TGTACA T^GTACA
3718 TGTACA
Van91I 4283 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4428 CCAGACTTTGG
XmnI 3862 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5549 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BclI BcnI BfaI
BfrI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmaCI XmaI