##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BbrPI BcgI BlnI BsaAI BsaBI
BseAI BsiWI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI DrdI
EcoRV FseI FspI KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI TaqI
TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 906 GGCGTC G[GA]^CG[CT]C
AflII 4986 CTTAAG C^TTAAG
AflIII 975 ACATGT A^C[GA][CT]GT
AhdI 5253 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3719 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5253 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3526 GGATCC G^GATCC
BfrI 4986 CTTAAG C^TTAAG
BglII 3827 AGATCT A^GATCT
BsaHI 906 GGCGTC G[GA]^CG[CT]C
BsiEI 5747 CGGCCG CG[GA][CT]^CG
BsmI 5058 GCATTC GAATGC(1/-1)
BspLU11I 975 ACATGT A^CATGT
BstAPI 3377 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 5747 CGGCCG C^GGCCG
Eam1105I 5253 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5747 CGGCCG C^GGCCG
Eco52I 5747 CGGCCG C^GGCCG
EcoT22I 2620 ATGCAT ATGCA^T
Hin1I 906 GGCGTC G[GA]^CG[CT]C
NotI 5746 GCGGCCGC GC^GGCCGC
NsiI 2620 ATGCAT ATGCA^T
SacI 3417 GAGCTC GAGCT^C
SapI 3221 GCTCTTC GCTCTTC(1/4)
SfiI 4503 GGCCTCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 5411 CCCGGG CCC^GGG
SphI 2444 GCATGC GCATG^C
XmaCI 5411 CCCGGG C^CCGGG
XmaI 5411 CCCGGG C^CCGGG
XmnI 3719 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 1260 CCTCAGG CC^T[ACGT]AGG
3631 CCTGAGG
Aor51HI 984 AGCGCT AGC^GCT
1540 AGCGCT
BciVI 137 GGATAC GTATCC(6/5)
3206 GTATCC
BglI 692 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4504 GCCTCAGCGGC
Bsp1407I 1221 TGTACA T^GTACA
3859 TGTACA
BspHI 605 TCATGA T^CATGA
2021 TCATGA
BsrGI 1221 TGTACA T^GTACA
3859 TGTACA
BstEII 1229 GGTGACC G^GT[ACGT]ACC
2794 GGTCACC
BstPI 1229 GGTGACC G^GT[ACGT]ACC
2794 GGTCACC
Bsu36I 1260 CCTCAGG CC^T[ACGT]AGG
3631 CCTGAGG
Eco47III 984 AGCGCT AGC^GCT
1540 AGCGCT
Eco81I 1260 CCTCAGG CC^T[ACGT]AGG
3631 CCTGAGG
EcoNI 1054 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4514 CCTGACACAGG
EcoO65I 1229 GGTGACC G^GT[ACGT]ACC
2794 GGTCACC
EcoRI 4388 GAATTC G^AATTC
5591 GAATTC
HgaI 884 GCGTC GACGC(5/10)
907 GCGTC
KspI 4447 CCGCGG CCGC^GG
5744 CCGCGG
MaeII 598 ACGT A^CGT
998 ACGT
RcaI 605 TCATGA T^CATGA
2021 TCATGA
SacII 4447 CCGCGG CCGC^GG
5744 CCGCGG
SspBI 1221 TGTACA T^GTACA
3859 TGTACA
XcmI 170 CCATCCCTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4653 CCAGGCGCATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I Van91I XhoII