##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AhdI AocI ApaI AscI Asp700I
Asp718I AspEI AspI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspLU11I BsrFI BssHII BstBI
BstEII BstPI Bsu36I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
FseI HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NgoMIV NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I RsrII
SacII SalI SapI SbfI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 4966 GACGTC GACGT^C
AccI 838 GTATAC GT^[AC][GT]AC
AccII 2421 CGCG CG^CG
Aor51HI 4998 AGCGCT AGC^GCT
AseI 2463 ATTAAT AT^TAAT
AsnI 2463 ATTAAT AT^TAAT
AviII 456 TGCGCA TGC^GCA
AvrII 1440 CCTAGG C^CTAGG
BamHI 4725 GGATCC G^GATCC
BbeI 2268 GGCGCC GGCGC^C
BbrPI 2850 CACGTG CAC^GTG
BbsI 3979 GAAGAC GAAGAC(2/6)
BcgI 3280 GCACCAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4539 GTATCC GTATCC(6/5)
BclI 1290 TGATCA T^GATCA
BlnI 1440 CCTAGG C^CTAGG
BpuAI 3979 GAAGAC GAAGAC(2/6)
BsiEI 440 CGATCG CG[GA][CT]^CG
BspHI 2879 TCATGA T^CATGA
Bst1107I 838 GTATAC GTA^TAC
BstUI 2421 CGCG CG^CG
BstZ17I 838 GTATAC GTA^TAC
Eco47III 4998 AGCGCT AGC^GCT
EcoNI 4174 CCTATCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 592 ATGCAT ATGCA^T
FbaI 1290 TGATCA T^GATCA
FspI 456 TGCGCA TGC^GCA
HgaI 3123 GCGTC GACGC(5/10)
HincII 3504 GTCAAC GT[CT]^[GA]AC
HindII 3504 GTCAAC GT[CT]^[GA]AC
KasI 2268 GGCGCC G^GCGCC
MvnI 2421 CGCG CG^CG
NarI 2268 GGCGCC GG^CGCC
NheI 4642 GCTAGC G^CTAGC
NsiI 592 ATGCAT ATGCA^T
PaeR7I 3131 CTCGAG C^TCGAG
PmaCI 2850 CACGTG CAC^GTG
PmlI 2850 CACGTG CAC^GTG
PshBI 2463 ATTAAT AT^TAAT
PvuI 440 CGATCG CGAT^CG
PvuII 4796 CAGCTG CAG^CTG
RcaI 2879 TCATGA T^CATGA
SacI 4607 GAGCTC GAGCT^C
SexAI 3282 ACCAGGT A^CC[AT]GGT
SfiI 5292 GGCCCTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2268 GGCGCC GGC^GCC
SmaI 2216 CCCGGG CCC^GGG
XbaI 169 TCTAGA T^CTAGA
XhoI 3131 CTCGAG C^TCGAG
XmaCI 2216 CCCGGG C^CCGGG
XmaI 2216 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 2268 GGCGCC G[GA]^CG[CT]C
4966 GACGTC
BglI 5011 GCCCGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5293 GCCCTCCTGGC
BsaAI 730 TACGTG [CT]AC^GT[GA]
2850 CACGTG
BsaHI 2268 GGCGCC G[GA]^CG[CT]C
4966 GACGTC
BsmBI 2316 GAGACG CGTCTC(1/5)
4893 GAGACG
BsmI 4529 GAATGC GAATGC(1/-1)
911 GCATTC
BspMI 2686 ACCTGC ACCTGC(4/8)
4824 ACCTGC
BsrBI 3919 GAGCGG CCGCTC(-3/-3)
4376 GAGCGG
BssSI 4702 CACGAG CACGAG(-5/-1)
3517 CTCGTG
EcoRV 1700 GATATC GAT^ATC
1864 GATATC
Hin1I 2268 GGCGCC G[GA]^CG[CT]C
4966 GACGTC
MspA1I 536 CAGCGG C[AC]G^C[GT]G
4796 CAGCTG
NcoI 3219 CCATGG C^CATGG
4419 CCATGG
PshAI 173 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5097 GACTGCAGTC
ScaI 2510 AGTACT AGT^ACT
5194 AGTACT
SphI 590 GCATGC GCATG^C
3948 GCATGC
StuI 2 AGGCCT AGG^CCT
5018 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BfrI BglII BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII