##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI Asp718I AviII BamHI BciVI
BclI BfrI BsaBI BsiWI BsmI
BspDI BspLU11I BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HpaI
KpnI MamI MfeI MluI MunI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I PvuI RsrII
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 1777 TCCGGA T^CCGGA
AcsI 4620 AAATTT [GA]^AATT[CT]
AflIII 2130 ACACGT A^C[GA][CT]GT
AhdI 1196 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1967 GTGCAC G^TGCAC
Aor51HI 1011 AGCGCT AGC^GCT
ApaLI 1967 GTGCAC G^TGCAC
ApoI 4620 AAATTT [GA]^AATT[CT]
AspEI 1196 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4365 CCTAGG C^CTAGG
BbeI 1336 GGCGCC GGCGC^C
BbrPI 2131 CACGTG CAC^GTG
BlnI 4365 CCTAGG C^CTAGG
BsaAI 2131 CACGTG [CT]AC^GT[GA]
BsaWI 1777 TCCGGA [AT]^CCGG[AT]
BseAI 1777 TCCGGA T^CCGGA
BspEI 1777 TCCGGA T^CCGGA
BspHI 2157 TCATGA T^CATGA
Eam1105I 1196 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1011 AGCGCT AGC^GCT
FseI 181 GGCCGGCC GGCCGG^CC
HindIII 480 AAGCTT A^AGCTT
KasI 1336 GGCGCC G^GCGCC
KspI 672 CCGCGG CCGC^GG
MroI 1777 TCCGGA T^CCGGA
NarI 1336 GGCGCC GG^CGCC
PaeR7I 1148 CTCGAG C^TCGAG
PmaCI 2131 CACGTG CAC^GTG
PmlI 2131 CACGTG CAC^GTG
PshAI 4385 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2157 TCATGA T^CATGA
SacII 672 CCGCGG CCGC^GG
SalI 459 GTCGAC G^TCGAC
SfoI 1336 GGCGCC GGC^GCC
XhoI 1148 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 427 GTCTAC GT^[AC][GT]AC
459 GTCGAC
AcyI 382 GGCGTC G[GA]^CG[CT]C
1336 GGCGCC
AseI 3385 ATTAAT AT^TAAT
3512 ATTAAT
AsnI 3385 ATTAAT AT^TAAT
3512 ATTAAT
BbsI 1096 GAAGAC GAAGAC(2/6)
3677 GAAGAC
BglII 2143 AGATCT A^GATCT
3050 AGATCT
BpuAI 1096 GAAGAC GAAGAC(2/6)
3677 GAAGAC
BsaHI 382 GGCGTC G[GA]^CG[CT]C
1336 GGCGCC
EagI 13 CGGCCG C^GGCCG
2107 CGGCCG
EclXI 13 CGGCCG C^GGCCG
2107 CGGCCG
Eco52I 13 CGGCCG C^GGCCG
2107 CGGCCG
Hin1I 382 GGCGTC G[GA]^CG[CT]C
1336 GGCGCC
NcoI 4352 CCATGG C^CATGG
5539 CCATGG
NdeI 4581 CATATG CA^TATG
5134 CATATG
PflMI 1737 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4738 CCAGAAGCTGG
PshBI 3385 ATTAAT AT^TAAT
3512 ATTAAT
SapI 802 GAAGAGC GCTCTTC(1/4)
923 GAAGAGC
SbfI 823 CCTGCAGG CCTGCA^GG
1189 CCTGCAGG
SexAI 810 ACCTGGT A^CC[AT]GGT
2941 ACCAGGT
SmaI 2402 CCCGGG CCC^GGG
3454 CCCGGG
SphI 5413 GCATGC GCATG^C
5516 GCATGC
Sse8387I 823 CCTGCAGG CCTGCA^GG
1189 CCTGCAGG
Van91I 1737 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4738 CCAGAAGCTGG
XbaI 2146 TCTAGA T^CTAGA
4717 TCTAGA
XmaCI 2402 CCCGGG C^CCGGG
3454 CCCGGG
XmaI 2402 CCCGGG C^CCGGG
3454 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NheI NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmnI