##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Alw44I
ApaLI AscI Asp700I Asp718I AspEI
AspI BbrPI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
FseI HpaI KpnI KspI MroI
NdeI NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 3965 GACGTC GACGT^C
AccII 1639 CGCG CG^CG
AclI 499 AACGTT AA^CGTT
AflII 1669 CTTAAG C^TTAAG
AflIII 1638 ACGCGT A^C[GA][CT]GT
Aor51HI 4814 AGCGCT AGC^GCT
AseI 3312 ATTAAT AT^TAAT
AsnI 3312 ATTAAT AT^TAAT
AviII 4736 TGCGCA TGC^GCA
AvrII 3822 CCTAGG C^CTAGG
BamHI 3051 GGATCC G^GATCC
BbeI 261 GGCGCC GGCGC^C
BbvCI 4262 CCTCAGC CCTCAGC(-5/-2)
BcgI 4251 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2432 GTATCC GTATCC(6/5)
BclI 2677 TGATCA T^GATCA
BfrI 1669 CTTAAG C^TTAAG
BglI 618 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 832 AGATCT A^GATCT
BlnI 3822 CCTAGG C^CTAGG
BsaBI 2672 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 2784 TGTACA T^GTACA
BsrGI 2784 TGTACA T^GTACA
BssSI 3872 CACGAG CACGAG(-5/-1)
BstBI 2026 TTCGAA TT^CGAA
BstEII 3831 GGTGACC G^GT[ACGT]ACC
BstPI 3831 GGTGACC G^GT[ACGT]ACC
BstUI 1639 CGCG CG^CG
DraI 1139 TTTAAA TTT^AAA
Eco47III 4814 AGCGCT AGC^GCT
EcoO65I 3831 GGTGACC G^GT[ACGT]ACC
FauI 5289 GCGGG CCCGC(4/6)
FbaI 2677 TGATCA T^GATCA
FspI 4736 TGCGCA TGC^GCA
KasI 261 GGCGCC G^GCGCC
MamI 2672 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1790 CAATTG C^AATTG
MluI 1638 ACGCGT A^CGCGT
MunI 1790 CAATTG C^AATTG
MvnI 1639 CGCG CG^CG
NaeI 5327 GCCGGC GCC^GGC
NarI 261 GGCGCC GG^CGCC
NgoMIV 5327 GCCGGC G^CCGGC
NspV 2026 TTCGAA TT^CGAA
PshAI 4707 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3312 ATTAAT AT^TAAT
Psp1406I 499 AACGTT AA^CGTT
SacI 95 GAGCTC GAGCT^C
SfoI 261 GGCGCC GGC^GCC
SfuI 2026 TTCGAA TT^CGAA
SphI 5019 GCATGC GCATG^C
SspBI 2784 TGTACA T^GTACA
TaqI 2027 TCGA T^CGA
TthHB8I 2027 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BsmI 2373 GCATTC GAATGC(1/-1)
3800 GCATTC
BspHI 2674 TCATGA T^CATGA
5430 TCATGA
BsrFI 2872 ACCGGC [GA]^CCGG[CT]
5327 GCCGGC
BstAPI 2964 GCAGCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3656 GCAGAAAGTGC
Cfr10I 2872 ACCGGC [GA]^CCGG[CT]
5327 GCCGGC
DraIII 15 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
4560 CACTGAGTG
EcoT22I 1086 ATGCAT ATGCA^T
3174 ATGCAT
HaeII 261 GGCGCC [GA]GCGC^[CT]
4814 AGCGCT
HincII 4714 GTCAAC GT[CT]^[GA]AC
4850 GTCAAC
HindII 4714 GTCAAC GT[CT]^[GA]AC
4850 GTCAAC
HindIII 2692 AAGCTT A^AGCTT
3733 AAGCTT
NsiI 1086 ATGCAT ATGCA^T
3174 ATGCAT
RcaI 2674 TCATGA T^CATGA
5430 TCATGA
SmaI 2337 CCCGGG CCC^GGG
2932 CCCGGG
SspI 4098 AATATT AAT^ATT
5486 AATATT
StuI 2411 AGGCCT AGG^CCT
3750 AGGCCT
XbaI 4083 TCTAGA T^CTAGA
4375 TCTAGA
XmaCI 2337 CCCGGG C^CCGGG
2932 CCCGGG
XmaI 2337 CCCGGG C^CCGGG
2932 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII