##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AviII BclI BfrI BglII
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRI EcoRV FbaI FseI
FspI HpaI KpnI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 1280 ACATGT A^C[GA][CT]GT
Alw44I 1744 GTGCAC G^TGCAC
AocI 4540 CCTCAGG CC^T[ACGT]AGG
ApaLI 1744 GTGCAC G^TGCAC
AvrII 4512 CCTAGG C^CTAGG
BamHI 1940 GGATCC G^GATCC
BbvCI 3138 GCTGAGG CCTCAGC(-5/-2)
BcgI 3475 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4512 CCTAGG C^CTAGG
BsaBI 2136 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 5026 GAATGC GAATGC(1/-1)
BspHI 1962 TCATGA T^CATGA
BspLU11I 1280 ACATGT A^CATGT
BsrDI 116 CATTGC GCAATG(2/0)
BssHII 3600 GCGCGC G^CGCGC
BssSI 1543 CACGAG CACGAG(-5/-1)
BstYI 1940 GGATCC [GA]^GATC[CT]
Bsu36I 4540 CCTCAGG CC^T[ACGT]AGG
DraI 3791 TTTAAA TTT^AAA
Eco81I 4540 CCTCAGG CC^T[ACGT]AGG
EcoT22I 5028 ATGCAT ATGCA^T
HincII 4069 GTCGAC GT[CT]^[GA]AC
HindII 4069 GTCGAC GT[CT]^[GA]AC
KspI 453 CCGCGG CCGC^GG
MamI 2136 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1940 GGATCC [GA]^GATC[CT]
NdeI 188 CATATG CA^TATG
NsiI 5028 ATGCAT ATGCA^T
RcaI 1962 TCATGA T^CATGA
SacII 453 CCGCGG CCGC^GG
SalI 4069 GTCGAC G^TCGAC
SbfI 310 CCTGCAGG CCTGCA^GG
Sse8387I 310 CCTGCAGG CCTGCA^GG
XhoII 1940 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 4151 AAATTT [GA]^AATT[CT]
4168 GAATTT
AhdI 908 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1990 GACCTGGAGTC
Aor51HI 2225 AGCGCT AGC^GCT
4528 AGCGCT
ApoI 4151 AAATTT [GA]^AATT[CT]
4168 GAATTT
AspEI 908 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1990 GACCTGGAGTC
BalI 1570 TGGCCA TGG^CCA
2358 TGGCCA
BbrPI 2151 CACGTG CAC^GTG
2251 CACGTG
BbsI 5004 GAAGAC GAAGAC(2/6)
1465 GTCTTC
BciVI 873 GTATCC GTATCC(6/5)
939 GTATCC
BlpI 657 GCTGAGC GC^T[ACGT]AGC
1804 GCTGAGC
Bpu1102I 657 GCTGAGC GC^T[ACGT]AGC
1804 GCTGAGC
BpuAI 5004 GAAGAC GAAGAC(2/6)
1465 GTCTTC
BsaI 3197 GGTCTC GGTCTC(1/5)
3591 GGTCTC
BstEII 2548 GGTCACC G^GT[ACGT]ACC
4423 GGTCACC
BstPI 2548 GGTCACC G^GT[ACGT]ACC
4423 GGTCACC
CelII 657 GCTGAGC GC^T[ACGT]AGC
1804 GCTGAGC
DraIII 167 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2968 CACCCGGTG
Eam1105I 908 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1990 GACCTGGAGTC
Eco47III 2225 AGCGCT AGC^GCT
4528 AGCGCT
EcoO65I 2548 GGTCACC G^GT[ACGT]ACC
4423 GGTCACC
HindIII 4023 AAGCTT A^AGCTT
4043 AAGCTT
MluNI 1570 TGGCCA TGG^CCA
2358 TGGCCA
MscI 1570 TGGCCA TGG^CCA
2358 TGGCCA
NaeI 225 GCCGGC GCC^GGC
3083 GCCGGC
NgoMIV 225 GCCGGC G^CCGGC
3083 GCCGGC
PmaCI 2151 CACGTG CAC^GTG
2251 CACGTG
PmlI 2151 CACGTG CAC^GTG
2251 CACGTG
SacI 3417 GAGCTC GAGCT^C
4103 GAGCTC
SapI 4192 GAAGAGC GCTCTTC(1/4)
4717 GCTCTTC
SfiI 502 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
781 GGCCCCTCTGGCC
SmlI 1324 CTCAAG C^T[CT][GA]AG
4455 CTTGAG
TfiI 3703 GATTC G^A[AT]TC
3721 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SspBI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XmaCI XmaI