##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI Eco47III EcoT22I
FbaI HpaI KspI MamI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1095 GACGTC GACGT^C
AccII 1237 CGCG CG^CG
AcsI 4157 GAATTC [GA]^AATT[CT]
AflIII 2367 ACATGT A^C[GA][CT]GT
AhdI 5317 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4157 GAATTC [GA]^AATT[CT]
AspEI 5317 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3529 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 719 TGCGCA TGC^GCA
BamHI 3351 GGATCC G^GATCC
BbeI 774 GGCGCC GGCGC^C
BbrPI 2174 CACGTG CAC^GTG
BsaAI 2174 CACGTG [CT]AC^GT[GA]
BsaWI 1908 ACCGGA [AT]^CCGG[AT]
BsmI 3560 GCATTC GAATGC(1/-1)
BspLU11I 2367 ACATGT A^CATGT
BstUI 1237 CGCG CG^CG
EagI 5113 CGGCCG C^GGCCG
Eam1105I 5317 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5113 CGGCCG C^GGCCG
Eco52I 5113 CGGCCG C^GGCCG
EcoRI 4157 GAATTC G^AATTC
EcoRV 3099 GATATC GAT^ATC
FseI 1707 GGCCGGCC GGCCGG^CC
FspI 719 TGCGCA TGC^GCA
HindIII 1169 AAGCTT A^AGCTT
KasI 774 GGCGCC G^GCGCC
MseI 5022 TTAA T^TAA
MvnI 1237 CGCG CG^CG
NarI 774 GGCGCC GG^CGCC
NheI 1542 GCTAGC G^CTAGC
PaeR7I 5098 CTCGAG C^TCGAG
PflFI 3529 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2174 CACGTG CAC^GTG
PmlI 2174 CACGTG CAC^GTG
SbfI 2766 CCTGCAGG CCTGCA^GG
SfiI 2280 GGCCACTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 774 GGCGCC GGC^GCC
SphI 904 GCATGC GCATG^C
Sse8387I 2766 CCTGCAGG CCTGCA^GG
Tru9I 5022 TTAA T^TAA
Tth111I 3529 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5098 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 1265 GGTACC G^GTACC
5258 GGTACC
Alw44I 2327 GTGCAC G^TGCAC
4368 GTGCAC
ApaLI 2327 GTGCAC G^TGCAC
4368 GTGCAC
Asp718I 1265 GGTACC G^GTACC
5258 GGTACC
AvrII 1547 CCTAGG C^CTAGG
3524 CCTAGG
BcgI 1252 CGATTTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4344 CGACCAGGATGC
BlnI 1547 CCTAGG C^CTAGG
3524 CCTAGG
BsiEI 5113 CGGCCG CG[GA][CT]^CG
5484 CGACCG
BsrDI 3413 CATTGC GCAATG(2/0)
2987 GCAATG
BstYI 3351 GGATCC [GA]^GATC[CT]
4949 GGATCT
DrdI 484 GACCCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5152 GACCTGCAAGTC
HaeII 774 GGCGCC [GA]GCGC^[CT]
5088 AGCGCC
HincII 2586 GTCAAC GT[CT]^[GA]AC
4283 GTTGAC
HindII 2586 GTCAAC GT[CT]^[GA]AC
4283 GTTGAC
KpnI 1265 GGTACC GGTAC^C
5258 GGTACC
MflI 3351 GGATCC [GA]^GATC[CT]
4949 GGATCT
NcoI 46 CCATGG C^CATGG
2025 CCATGG
SmaI 4338 CCCGGG CCC^GGG
4926 CCCGGG
Tsp509I 1189 AATT ^AATT
4158 AATT
XhoII 3351 GGATCC [GA]^GATC[CT]
4949 GGATCT
XmaCI 4338 CCCGGG C^CCGGG
4926 CCCGGG
XmaI 4338 CCCGGG C^CCGGG
4926 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI