##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI Asp700I Asp718I
AviII BcgI BciVI BclI BfrI
BglII BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BstAPI BstBI ClaI CpoI
DraI DrdI Eco47III EcoRI EcoT22I
FbaI FspI KpnI KspI MamI
MroI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1647 GAATTT [GA]^AATT[CT]
AflIII 3808 ACGCGT A^C[GA][CT]GT
ApoI 1647 GAATTT [GA]^AATT[CT]
AseI 159 ATTAAT AT^TAAT
AsnI 159 ATTAAT AT^TAAT
AspI 1407 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 948 CACGTG CAC^GTG
BbvCI 4145 CCTCAGC CCTCAGC(-5/-2)
BsaAI 948 CACGTG [CT]AC^GT[GA]
BsaWI 1489 TCCGGT [AT]^CCGG[AT]
BsmBI 3710 GAGACG CGTCTC(1/5)
BsmI 565 GCATTC GAATGC(1/-1)
BssSI 2192 CACGAG CACGAG(-5/-1)
BstEII 2415 GGTTACC G^GT[ACGT]ACC
BstPI 2415 GGTTACC G^GT[ACGT]ACC
EcoO65I 2415 GGTTACC G^GT[ACGT]ACC
EcoRV 4223 GATATC GAT^ATC
FseI 1564 GGCCGGCC GGCCGG^CC
HincII 246 GTTAAC GT[CT]^[GA]AC
HindII 246 GTTAAC GT[CT]^[GA]AC
HpaI 246 GTTAAC GTT^AAC
MfeI 5129 CAATTG C^AATTG
MluI 3808 ACGCGT A^CGCGT
MunI 5129 CAATTG C^AATTG
NheI 4207 GCTAGC G^CTAGC
NspI 1863 GCATGT [GA]CATG^[CT]
PflFI 1407 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 948 CACGTG CAC^GTG
PmlI 948 CACGTG CAC^GTG
PshAI 3525 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 159 ATTAAT AT^TAAT
SapI 2486 GCTCTTC GCTCTTC(1/4)
SexAI 1408 ACCTGGT A^CC[AT]GGT
Tth111I 1407 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 3216 GTAC GT^AC
4688 GTAC
AhdI 1893 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3366 GACTTGGTGTC
Alw44I 2405 GTGCAC G^TGCAC
3445 GTGCAC
ApaLI 2405 GTGCAC G^TGCAC
3445 GTGCAC
AspEI 1893 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3366 GACTTGGTGTC
BamHI 347 GGATCC G^GATCC
4436 GGATCC
BbeI 1246 GGCGCC GGCGC^C
3508 GGCGCC
BbsI 1438 GAAGAC GAAGAC(2/6)
2282 GAAGAC
BpuAI 1438 GAAGAC GAAGAC(2/6)
2282 GAAGAC
BsrDI 241 GCAATG GCAATG(2/0)
271 GCAATG
Bst1107I 210 GTATAC GTA^TAC
833 GTATAC
BstZ17I 210 GTATAC GTA^TAC
833 GTATAC
DraIII 377 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1667 CACCCTGTG
EagI 1567 CGGCCG C^GGCCG
2714 CGGCCG
Eam1105I 1893 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3366 GACTTGGTGTC
EclXI 1567 CGGCCG C^GGCCG
2714 CGGCCG
Eco52I 1567 CGGCCG C^GGCCG
2714 CGGCCG
HaeII 1246 GGCGCC [GA]GCGC^[CT]
3508 GGCGCC
HindIII 1057 AAGCTT A^AGCTT
4812 AAGCTT
KasI 1246 GGCGCC G^GCGCC
3508 GGCGCC
MaeII 949 ACGT A^CGT
2702 ACGT
NarI 1246 GGCGCC GG^CGCC
3508 GGCGCC
PflMI 4550 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4786 CCAGGCCCTGG
RsaI 3216 GTAC GT^AC
4688 GTAC
SfoI 1246 GGCGCC GGC^GCC
3508 GGCGCC
TaqI 3572 TCGA T^CGA
3738 TCGA
Tsp509I 1648 AATT ^AATT
5130 AATT
TthHB8I 3572 TCGA T^CGA
3738 TCGA
Van91I 4550 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4786 CCAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcyI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I TspRI
XcmI XhoII XmaCI XmaI