##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AhdI Aor51HI AscI AspEI
AvrII BclI BlnI BsaBI BsiWI
BspDI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI Eam1105I Eco47III EcoRV
FbaI FseI HpaI MamI MfeI
MunI NheI NotI NruI NspV
PacI PmeI PshAI PvuI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 3226 GGTACC G^GTACC
AclI 4675 AACGTT AA^CGTT
AflII 1093 CTTAAG C^TTAAG
AgeI 1600 ACCGGT A^CCGGT
AseI 3994 ATTAAT AT^TAAT
AsnI 3994 ATTAAT AT^TAAT
Asp718I 3226 GGTACC G^GTACC
AspI 4174 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4982 TGCGCA TGC^GCA
BbrPI 2428 CACGTG CAC^GTG
BciVI 5356 GGATAC GTATCC(6/5)
BfrI 1093 CTTAAG C^TTAAG
BglII 4028 AGATCT A^GATCT
BlpI 3427 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3427 GCTGAGC GC^T[ACGT]AGC
BspHI 4886 TCATGA T^CATGA
BspLU11I 3293 ACATGT A^CATGT
BsrDI 3689 GCAATG GCAATG(2/0)
BstEII 2804 GGTGACC G^GT[ACGT]ACC
BstPI 2804 GGTGACC G^GT[ACGT]ACC
CelII 3427 GCTGAGC GC^T[ACGT]AGC
CpoI 1675 CGGACCG CG^G[AT]CCG
EagI 2164 CGGCCG C^GGCCG
EclXI 2164 CGGCCG C^GGCCG
Eco52I 2164 CGGCCG C^GGCCG
EcoO65I 2804 GGTGACC G^GT[ACGT]ACC
EcoRI 911 GAATTC G^AATTC
EcoT22I 809 ATGCAT ATGCA^T
FspI 4982 TGCGCA TGC^GCA
HindIII 1753 AAGCTT A^AGCTT
KpnI 3226 GGTACC GGTAC^C
KspI 1813 CCGCGG CCGC^GG
NdeI 761 CATATG CA^TATG
NsiI 809 ATGCAT ATGCA^T
PaeR7I 3345 CTCGAG C^TCGAG
PflFI 4174 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1600 ACCGGT A^CCGGT
PmaCI 2428 CACGTG CAC^GTG
PmlI 2428 CACGTG CAC^GTG
PshBI 3994 ATTAAT AT^TAAT
Psp1406I 4675 AACGTT AA^CGTT
RcaI 4886 TCATGA T^CATGA
RsrII 1675 CGGACCG CG^G[AT]CCG
SacII 1813 CCGCGG CCGC^GG
SalI 4102 GTCGAC G^TCGAC
SapI 2781 GCTCTTC GCTCTTC(1/4)
SexAI 491 ACCTGGT A^CC[AT]GGT
SspI 637 AATATT AAT^ATT
Tth111I 4174 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3345 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3568 GTCTAC GT^[AC][GT]AC
4102 GTCGAC
AccIII 1341 TCCGGA T^CCGGA
1688 TCCGGA
Asp700I 3982 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4942 GAATCCATTC
BamHI 1186 GGATCC G^GATCC
3889 GGATCC
BbeI 5175 GGCGCC GGCGC^C
5211 GGCGCC
BcgI 359 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3812 GCAAGATTGTCG
BseAI 1341 TCCGGA T^CCGGA
1688 TCCGGA
BsiEI 2164 CGGCCG CG[GA][CT]^CG
4817 CGGTCG
BsmI 880 GAATGC GAATGC(1/-1)
846 GCATTC
BspEI 1341 TCCGGA T^CCGGA
1688 TCCGGA
BsrBI 2557 GAGCGG CCGCTC(-3/-3)
2610 GAGCGG
DraIII 1542 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2677 CACAGGGTG
KasI 5175 GGCGCC G^GCGCC
5211 GGCGCC
MluI 3451 ACGCGT A^CGCGT
3560 ACGCGT
MroI 1341 TCCGGA T^CCGGA
1688 TCCGGA
NarI 5175 GGCGCC GG^CGCC
5211 GGCGCC
NcoI 224 CCATGG C^CATGG
2722 CCATGG
SacI 2656 GAGCTC GAGCT^C
4216 GAGCTC
SbfI 4035 CCTGCAGG CCTGCA^GG
4524 CCTGCAGG
SfoI 5175 GGCGCC GGC^GCC
5211 GGCGCC
SmaI 2975 CCCGGG CCC^GGG
3595 CCCGGG
SmlI 1093 CTTAAG C^T[CT][GA]AG
3345 CTCGAG
SphI 720 GCATGC GCATG^C
2455 GCATGC
Sse8387I 4035 CCTGCAGG CCTGCA^GG
4524 CCTGCAGG
XbaI 1233 TCTAGA T^CTAGA
2375 TCTAGA
XmaCI 2975 CCCGGG C^CCGGG
3595 CCCGGG
XmaI 2975 CCCGGG C^CCGGG
3595 CCCGGG
XmnI 3982 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4942 GAATCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII