##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII BcgI
BfrI BsaBI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EcoRI
EcoRV FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MunI
NotI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 4998 GACGTC GACGT^C
Alw44I 2441 GTGCAC G^TGCAC
ApaLI 2441 GTGCAC G^TGCAC
AvrII 1880 CCTAGG C^CTAGG
BamHI 4082 GGATCC G^GATCC
BciVI 1585 GGATAC GTATCC(6/5)
BclI 2971 TGATCA T^GATCA
BglII 5688 AGATCT A^GATCT
BlnI 1880 CCTAGG C^CTAGG
BsmBI 4508 CGTCTC CGTCTC(1/5)
BsrBI 5023 CCGCTC CCGCTC(-3/-3)
BsrFI 4925 GCCGGC [GA]^CCGG[CT]
BssHII 575 GCGCGC G^CGCGC
BstEII 5509 GGTCACC G^GT[ACGT]ACC
BstPI 5509 GGTCACC G^GT[ACGT]ACC
Cfr10I 4925 GCCGGC [GA]^CCGG[CT]
DraI 1906 TTTAAA TTT^AAA
EcoO65I 5509 GGTCACC G^GT[ACGT]ACC
FbaI 2971 TGATCA T^GATCA
HincII 5337 GTCAAC GT[CT]^[GA]AC
HindII 5337 GTCAAC GT[CT]^[GA]AC
NaeI 4925 GCCGGC GCC^GGC
NdeI 1430 CATATG CA^TATG
NgoMIV 4925 GCCGGC G^CCGGC
NheI 896 GCTAGC G^CTAGC
NruI 828 TCGCGA TCG^CGA
PaeR7I 4500 CTCGAG C^TCGAG
SphI 4019 GCATGC GCATG^C
XhoI 4500 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 497 GTCTAC GT^[AC][GT]AC
2992 GTAGAC
AccIII 2178 TCCGGA T^CCGGA
4908 TCCGGA
AflIII 881 ACACGT A^C[GA][CT]GT
2794 ACGTGT
AhdI 4374 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5460 GACCGTGGGTC
AspEI 4374 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5460 GACCGTGGGTC
AspI 1897 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3598 GACTTGGTC
BbrPI 882 CACGTG CAC^GTG
2793 CACGTG
BsaAI 882 CACGTG [CT]AC^GT[GA]
2793 CACGTG
BseAI 2178 TCCGGA T^CCGGA
4908 TCCGGA
BsiEI 4664 CGGCCG CG[GA][CT]^CG
4927 CGGCCG
BsmI 5553 GAATGC GAATGC(1/-1)
1707 GCATTC
BspEI 2178 TCCGGA T^CCGGA
4908 TCCGGA
BssSI 2267 CACGAG CACGAG(-5/-1)
4743 CACGAG
DraIII 1598 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
3259 CACTCTGTG
EagI 4664 CGGCCG C^GGCCG
4927 CGGCCG
Eam1105I 4374 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5460 GACCGTGGGTC
EclXI 4664 CGGCCG C^GGCCG
4927 CGGCCG
Eco52I 4664 CGGCCG C^GGCCG
4927 CGGCCG
EcoT22I 1224 ATGCAT ATGCA^T
5555 ATGCAT
MroI 2178 TCCGGA T^CCGGA
4908 TCCGGA
NsiI 1224 ATGCAT ATGCA^T
5555 ATGCAT
PflFI 1897 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3598 GACTTGGTC
PmaCI 882 CACGTG CAC^GTG
2793 CACGTG
PmlI 882 CACGTG CAC^GTG
2793 CACGTG
Tth111I 1897 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3598 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI