##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AviII BciVI BclI BfrI
BglII BsaBI BsiWI BspDI BspLU11I
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I Eco47III EcoRV FbaI
FseI FspI HindIII HpaI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
TfiI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1552 GGTACC G^GTACC
AccIII 1141 TCCGGA T^CCGGA
AcsI 3318 GAATTC [GA]^AATT[CT]
Alw44I 365 GTGCAC G^TGCAC
ApaLI 365 GTGCAC G^TGCAC
ApoI 3318 GAATTC [GA]^AATT[CT]
Asp700I 4160 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1552 GGTACC G^GTACC
BbeI 833 GGCGCC GGCGC^C
BsaWI 1141 TCCGGA [AT]^CCGG[AT]
BseAI 1141 TCCGGA T^CCGGA
BsmI 1647 GAATGC GAATGC(1/-1)
Bsp1407I 1719 TGTACA T^GTACA
BspEI 1141 TCCGGA T^CCGGA
BspHI 3895 TCATGA T^CATGA
BsrBI 1773 GAGCGG CCGCTC(-3/-3)
BsrDI 4045 CATTGC GCAATG(2/0)
BsrGI 1719 TGTACA T^GTACA
BssHII 1200 GCGCGC G^CGCGC
BssSI 4701 CTCGTG CACGAG(-5/-1)
DrdI 4665 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1164 CGGCCG C^GGCCG
EclXI 1164 CGGCCG C^GGCCG
Eco52I 1164 CGGCCG C^GGCCG
EcoRI 3318 GAATTC G^AATTC
EcoT22I 1649 ATGCAT ATGCA^T
HincII 296 GTCGAC GT[CT]^[GA]AC
HindII 296 GTCGAC GT[CT]^[GA]AC
KasI 833 GGCGCC G^GCGCC
KpnI 1552 GGTACC GGTAC^C
KspI 483 CCGCGG CCGC^GG
MroI 1141 TCCGGA T^CCGGA
NaeI 2505 GCCGGC GCC^GGC
NarI 833 GGCGCC GG^CGCC
NgoMIV 2505 GCCGGC G^CCGGC
NsiI 1649 ATGCAT ATGCA^T
NspI 5140 ACATGC [GA]CATG^[CT]
RcaI 3895 TCATGA T^CATGA
SacII 483 CCGCGG CCGC^GG
SalI 296 GTCGAC G^TCGAC
SapI 2414 GCTCTTC GCTCTTC(1/4)
SfoI 833 GGCGCC GGC^GCC
SspBI 1719 TGTACA T^GTACA
XmnI 4160 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BbrPI 4815 CACGTG CAC^GTG
5106 CACGTG
BbsI 3584 GTCTTC GAAGAC(2/6)
4569 GTCTTC
BbvCI 2857 GCTGAGG CCTCAGC(-5/-2)
3902 GCTGAGG
BcgI 1075 CGAGGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2036 GCACTCACCTCG
BpuAI 3584 GTCTTC GAAGAC(2/6)
4569 GTCTTC
BsaAI 4815 CACGTG [CT]AC^GT[GA]
5106 CACGTG
BsmBI 2584 CGTCTC CGTCTC(1/5)
4113 CGTCTC
BsrFI 2505 GCCGGC [GA]^CCGG[CT]
3633 GCCGGT
Cfr10I 2505 GCCGGC [GA]^CCGG[CT]
3633 GCCGGT
DraIII 1705 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4574 CACTGAGTG
NcoI 852 CCATGG C^CATGG
1904 CCATGG
PmaCI 4815 CACGTG CAC^GTG
5106 CACGTG
PmlI 4815 CACGTG CAC^GTG
5106 CACGTG
SfiI 340 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4163 GGCCTTCCAGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII AvrII
BalI BamHI BanI BanII BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PaeR7I
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmaI
SmlI Sse8387I StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI