##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
AclI AflII AscI AseI AsnI
BclI BfrI BglII BspDI Bst1107I
BstZ17I ClaI CpoI EcoRI EcoRV
FbaI FseI HindIII HpaI MluI
NotI NruI PacI PaeR7I PmeI
PshBI Psp1406I PvuI RsrII SapI
SfiI SgrAI SnaBI SpeI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 3273 GGTACC G^GTACC
AccIII 3907 TCCGGA T^CCGGA
AgeI 4825 ACCGGT A^CCGGT
AhdI 406 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4734 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3273 GGTACC G^GTACC
AspEI 406 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2151 CCTAGG C^CTAGG
BciVI 1052 GGATAC GTATCC(6/5)
BlnI 2151 CCTAGG C^CTAGG
BseAI 3907 TCCGGA T^CCGGA
BsiWI 947 CGTACG C^GTACG
BsmI 1205 GAATGC GAATGC(1/-1)
BspEI 3907 TCCGGA T^CCGGA
BspHI 4583 TCATGA T^CATGA
BspLU11I 3114 ACATGT A^CATGT
BsrDI 1466 GCAATG GCAATG(2/0)
BssHII 2332 GCGCGC G^CGCGC
BstBI 1202 TTCGAA TT^CGAA
DraIII 1691 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1669 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 406 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2675 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1207 ATGCAT ATGCA^T
KpnI 3273 GGTACC GGTAC^C
MfeI 907 CAATTG C^AATTG
MroI 3907 TCCGGA T^CCGGA
MunI 907 CAATTG C^AATTG
NcoI 2580 CCATGG C^CATGG
NdeI 3671 CATATG CA^TATG
NheI 2751 GCTAGC G^CTAGC
NsiI 1207 ATGCAT ATGCA^T
NspV 1202 TTCGAA TT^CGAA
PinAI 4825 ACCGGT A^CCGGT
PshAI 4269 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4583 TCATGA T^CATGA
SbfI 1816 CCTGCAGG CCTGCA^GG
SexAI 112 ACCTGGT A^CC[AT]GGT
SfuI 1202 TTCGAA TT^CGAA
SphI 2509 GCATGC GCATG^C
Sse8387I 1816 CCTGCAGG CCTGCA^GG
SspI 4867 AATATT AAT^ATT
TfiI 4154 GATTC G^A[AT]TC
XmnI 4734 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 69 GACGTC GACGT^C
3972 GACGTC
AocI 103 CCTCAGG CC^T[ACGT]AGG
1681 CCTGAGG
AspI 379 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3837 GACACAGTC
AviII 1809 TGCGCA TGC^GCA
4748 TGCGCA
BamHI 2761 GGATCC G^GATCC
4425 GGATCC
BbrPI 496 CACGTG CAC^GTG
3285 CACGTG
BcgI 1267 GCAGCGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1749 GCAGGGAGCTCG
BsaWI 3907 TCCGGA [AT]^CCGG[AT]
4825 ACCGGT
Bsp1407I 2905 TGTACA T^GTACA
4436 TGTACA
BsrGI 2905 TGTACA T^GTACA
4436 TGTACA
BstEII 2439 GGTCACC G^GT[ACGT]ACC
3131 GGTGACC
BstPI 2439 GGTCACC G^GT[ACGT]ACC
3131 GGTGACC
BstXI 337 CCACCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2259 CCACCAAGGTGG
Bsu36I 103 CCTCAGG CC^T[ACGT]AGG
1681 CCTGAGG
Eco81I 103 CCTCAGG CC^T[ACGT]AGG
1681 CCTGAGG
EcoO65I 2439 GGTCACC G^GT[ACGT]ACC
3131 GGTGACC
FspI 1809 TGCGCA TGC^GCA
4748 TGCGCA
NaeI 370 GCCGGC GCC^GGC
2294 GCCGGC
NgoMIV 370 GCCGGC G^CCGGC
2294 GCCGGC
PflFI 379 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3837 GACACAGTC
PmaCI 496 CACGTG CAC^GTG
3285 CACGTG
PmlI 496 CACGTG CAC^GTG
3285 CACGTG
SacI 1754 GAGCTC GAGCT^C
4184 GAGCTC
SalI 3834 GTCGAC G^TCGAC
3975 GTCGAC
ScaI 1554 AGTACT AGT^ACT
2923 AGTACT
SspBI 2905 TGTACA T^GTACA
4436 TGTACA
Tth111I 379 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3837 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI