##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AvaII
AviII BamHI BbeI BcgI BcnI
BlpI Bpu1102I BsaBI BsaHI BsiEI
BsiWI BsmBI BsmI Bsp1407I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoT22I FauI FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MamI MluI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmeI PpuMI PshAI PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 3423 TCCGGA T^CCGGA
AclI 3689 AACGTT AA^CGTT
AflIII 3157 ACATGT A^C[GA][CT]GT
Asp700I 582 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 364 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3393 CTCGAG C^[CT]CG[GA]G
AvrII 2707 CCTAGG C^CTAGG
BanI 2849 GGTGCC G^G[CT][GA]CC
BbrPI 2751 CACGTG CAC^GTG
BglI 493 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1480 AGATCT A^GATCT
BlnI 2707 CCTAGG C^CTAGG
BsaWI 3423 TCCGGA [AT]^CCGG[AT]
BseAI 3423 TCCGGA T^CCGGA
BseRI 1476 GAGGAG GAGGAG(10/8)
BsoBI 3393 CTCGAG C^[CT]CG[GA]G
BspDI 2885 ATCGAT AT^CGAT
BspEI 3423 TCCGGA T^CCGGA
BspLU11I 3157 ACATGT A^CATGT
BsrDI 2739 GCAATG GCAATG(2/0)
BssSI 3501 CACGAG CACGAG(-5/-1)
BstEII 3809 GGTTACC G^GT[ACGT]ACC
BstPI 3809 GGTTACC G^GT[ACGT]ACC
CfoI 2237 GCGC GCG^C
ClaI 2885 ATCGAT AT^CGAT
DraIII 1892 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1275 GAAGAG CTCTTC(1/4)
EcoO65I 3809 GGTTACC G^GT[ACGT]ACC
EcoRI 2658 GAATTC G^AATTC
EcoRV 532 GATATC GAT^ATC
HhaI 2237 GCGC GCG^C
HinP1I 2237 GCGC G^CGC
HindIII 859 AAGCTT A^AGCTT
Ksp632I 1275 GAAGAG CTCTTC(1/4)
MfeI 953 CAATTG C^AATTG
MroI 3423 TCCGGA T^CCGGA
MspA1I 3470 CAGCTG C[AC]G^C[GT]G
MunI 953 CAATTG C^AATTG
PaeR7I 3393 CTCGAG C^TCGAG
PflFI 364 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2751 CACGTG CAC^GTG
PmlI 2751 CACGTG CAC^GTG
Psp1406I 3689 AACGTT AA^CGTT
PvuII 3470 CAGCTG CAG^CTG
SapI 1275 GAAGAGC GCTCTTC(1/4)
SpeI 2534 ACTAGT A^CTAGT
StuI 2704 AGGCCT AGG^CCT
Tth111I 364 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3393 CTCGAG C^TCGAG
XmnI 582 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 440 CTTAAG C^TTAAG
707 CTTAAG
Alw44I 3078 GTGCAC G^TGCAC
3927 GTGCAC
AlwNI 3051 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3384 CAGAAGCTG
ApaLI 3078 GTGCAC G^TGCAC
3927 GTGCAC
AseI 1697 ATTAAT AT^TAAT
2502 ATTAAT
AsnI 1697 ATTAAT AT^TAAT
2502 ATTAAT
BalI 144 TGGCCA TGG^CCA
1663 TGGCCA
BbsI 1234 GAAGAC GAAGAC(2/6)
3539 GAAGAC
BciVI 1221 GTATCC GTATCC(6/5)
1317 GTATCC
BclI 2866 TGATCA T^GATCA
3139 TGATCA
BfrI 440 CTTAAG C^TTAAG
707 CTTAAG
BpuAI 1234 GAAGAC GAAGAC(2/6)
3539 GAAGAC
BsaAI 2425 TACGTG [CT]AC^GT[GA]
2751 CACGTG
BspHI 1566 TCATGA T^CATGA
3136 TCATGA
EaeI 144 TGGCCA [CT]^GGCC[GA]
1663 TGGCCA
FbaI 2866 TGATCA T^GATCA
3139 TGATCA
HapII 98 CCGG C^CGG
3424 CCGG
HgaI 1249 GACGC GACGC(5/10)
3542 GACGC
HincII 958 GTCAAC GT[CT]^[GA]AC
3655 GTCAAC
HindII 958 GTCAAC GT[CT]^[GA]AC
3655 GTCAAC
HpaII 98 CCGG C^CGG
3424 CCGG
MluNI 144 TGGCCA TGG^CCA
1663 TGGCCA
MscI 144 TGGCCA TGG^CCA
1663 TGGCCA
MspI 98 CCGG C^CGG
3424 CCGG
NcoI 497 CCATGG C^CATGG
2934 CCATGG
PshBI 1697 ATTAAT AT^TAAT
2502 ATTAAT
RcaI 1566 TCATGA T^CATGA
3136 TCATGA
SacI 412 GAGCTC GAGCT^C
3419 GAGCTC
XcmI 3090 CCAGATTTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3628 CCAGCATGGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BanII BbvCI BbvI BfaI
BmyI BpmI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII