##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AhdI AlwNI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BcgI BciVI BglII
BlpI Bpu1102I BsiWI BspDI BspHI
BsrDI BstBI BstEII BstPI CelII
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoRI EcoT22I FspI HpaI KpnI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI PvuI
RcaI RsrII SalI SbfI ScaI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 4090 GTATAC GT^[AC][GT]AC
AccIII 319 TCCGGA T^CCGGA
AclI 3548 AACGTT AA^CGTT
AflII 3472 CTTAAG C^TTAAG
AgeI 1242 ACCGGT A^CCGGT
Asp700I 3864 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 2291 CACGTG CAC^GTG
BclI 4115 TGATCA T^GATCA
BfrI 3472 CTTAAG C^TTAAG
BpmI 3738 CTCCAG CTGGAG(16/14)
BsaAI 2291 CACGTG [CT]AC^GT[GA]
BsaBI 190 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3404 GAGACC GGTCTC(1/5)
BseAI 319 TCCGGA T^CCGGA
BsmBI 496 GAGACG CGTCTC(1/5)
BsmI 836 GCATTC GAATGC(1/-1)
Bsp1407I 108 TGTACA T^GTACA
BspEI 319 TCCGGA T^CCGGA
BsrBI 1400 GAGCGG CCGCTC(-3/-3)
BsrGI 108 TGTACA T^GTACA
Bst1107I 4090 GTATAC GTA^TAC
BstAPI 367 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4090 GTATAC GTA^TAC
DraIII 368 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1520 CGGCCG C^GGCCG
EclXI 1520 CGGCCG C^GGCCG
Eco52I 1520 CGGCCG C^GGCCG
EcoRV 3795 GATATC GAT^ATC
FbaI 4115 TGATCA T^GATCA
FseI 2137 GGCCGGCC GGCCGG^CC
HincII 911 GTCAAC GT[CT]^[GA]AC
HindII 911 GTCAAC GT[CT]^[GA]AC
HindIII 3883 AAGCTT A^AGCTT
MamI 190 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 319 TCCGGA T^CCGGA
MslI 4262 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2138 GCCGGC GCC^GGC
NdeI 112 CATATG CA^TATG
NgoMIV 2138 GCCGGC G^CCGGC
PflMI 650 CCAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1242 ACCGGT A^CCGGT
PmaCI 2291 CACGTG CAC^GTG
PmlI 2291 CACGTG CAC^GTG
Psp1406I 3548 AACGTT AA^CGTT
SacI 3622 GAGCTC GAGCT^C
SapI 1454 GCTCTTC GCTCTTC(1/4)
SexAI 369 ACCTGGT A^CC[AT]GGT
SfiI 4211 GGCCTGTAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2801 ACTAGT A^CTAGT
SspBI 108 TGTACA T^GTACA
Van91I 650 CCAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1849 TCTAGA T^CTAGA
XmnI 3864 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 985 GACGTC GACGT^C
1904 GACGTC
AcsI 54 AAATTC [GA]^AATT[CT]
3865 AAATTT
AfaI 109 GTAC GT^AC
1425 GTAC
Alw44I 1627 GTGCAC G^TGCAC
1945 GTGCAC
AocI 419 CCTAAGG CC^T[ACGT]AGG
2669 CCTCAGG
ApaI 252 GGGCCC GGGCC^C
2245 GGGCCC
ApaLI 1627 GTGCAC G^TGCAC
1945 GTGCAC
ApoI 54 AAATTC [GA]^AATT[CT]
3865 AAATTT
BalI 1930 TGGCCA TGG^CCA
2437 TGGCCA
BbvCI 305 CCTCAGC CCTCAGC(-5/-2)
4327 GCTGAGG
BsiEI 1520 CGGCCG CG[GA][CT]^CG
2101 CGACCG
BssHII 2110 GCGCGC G^CGCGC
2112 GCGCGC
BssSI 1635 CACGAG CACGAG(-5/-1)
2078 CTCGTG
BstYI 1468 AGATCC [GA]^GATC[CT]
1807 AGATCC
Bsu36I 419 CCTAAGG CC^T[ACGT]AGG
2669 CCTCAGG
DrdI 498 GACGAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1383 GACAGCGGCGTC
Eco81I 419 CCTAAGG CC^T[ACGT]AGG
2669 CCTCAGG
EcoNI 2515 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2665 CCTGCCTCAGG
MflI 1468 AGATCC [GA]^GATC[CT]
1807 AGATCC
MluNI 1930 TGGCCA TGG^CCA
2437 TGGCCA
MscI 1930 TGGCCA TGG^CCA
2437 TGGCCA
NcoI 855 CCATGG C^CATGG
2055 CCATGG
RsaI 109 GTAC GT^AC
1425 GTAC
XhoII 1468 AGATCC [GA]^GATC[CT]
1807 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AlwI AspHI AvaI AvaII AvrII
BanI BanII BbeI BbsI BbvI
BcnI BfaI BglI BlnI BmyI
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI