##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AvaI AviII
BanI BbeI BbrPI BbvCI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BssHII
BstBI BstEII BstPI Bsu36I CelII
ClaI CpoI DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FauI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 4342 CGCG CG^CG
AflII 4078 CTTAAG C^TTAAG
AhdI 4473 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2046 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4473 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 387 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2690 TGGCCA TGG^CCA
BamHI 1729 GGATCC G^GATCC
BcgI 1772 CGAAGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 892 GGATAC GTATCC(6/5)
BfrI 4078 CTTAAG C^TTAAG
BglI 4309 GCCCGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4415 AGATCT A^GATCT
BsaAI 3943 TACGTA [CT]AC^GT[GA]
BseRI 1649 CTCCTC GAGGAG(10/8)
BsgI 3844 CTGCAC GTGCAG(16/14)
Bsp1407I 811 TGTACA T^GTACA
BspHI 74 TCATGA T^CATGA
BsrFI 197 GCCGGT [GA]^CCGG[CT]
BsrGI 811 TGTACA T^GTACA
BssSI 5067 CACGAG CACGAG(-5/-1)
Bst1107I 3768 GTATAC GTA^TAC
BstUI 4342 CGCG CG^CG
BstZ17I 3768 GTATAC GTA^TAC
CfoI 4343 GCGC GCG^C
Cfr10I 197 GCCGGT [GA]^CCGG[CT]
EaeI 2690 TGGCCA [CT]^GGCC[GA]
Eam1105I 4473 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4643 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 4343 GCGC GCG^C
HinP1I 4343 GCGC G^CGC
HincII 3142 GTCAAC GT[CT]^[GA]AC
HindII 3142 GTCAAC GT[CT]^[GA]AC
MluNI 2690 TGGCCA TGG^CCA
MscI 2690 TGGCCA TGG^CCA
MvnI 4342 CGCG CG^CG
NspI 3887 ACATGC [GA]CATG^[CT]
PacI 2379 TTAATTAA TTAAT^TAA
PflFI 387 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 74 TCATGA T^CATGA
SacI 854 GAGCTC GAGCT^C
SapI 3220 GCTCTTC GCTCTTC(1/4)
SnaBI 3943 TACGTA TAC^GTA
SspBI 811 TGTACA T^GTACA
StuI 1516 AGGCCT AGG^CCT
TaqI 2266 TCGA T^CGA
Tth111I 387 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2266 TCGA T^CGA
XmnI 2046 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 2100 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
4788 CAGGGCCTG
AvaII 146 GGACC G^G[AT]CC
3289 GGACC
BbsI 838 GAAGAC GAAGAC(2/6)
1773 GAAGAC
BpuAI 838 GAAGAC GAAGAC(2/6)
1773 GAAGAC
BsmBI 5114 CGTCTC CGTCTC(1/5)
4083 GAGACG
BsrDI 4158 GCAATG GCAATG(2/0)
4191 GCAATG
BstAPI 4169 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4318 GCAGAGATTGC
DraIII 1710 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2762 CACAGTGTG
MaeII 388 ACGT A^CGT
3944 ACGT
MspA1I 2851 CAGCTG C[AC]G^C[GT]G
4858 CAGCTG
NdeI 2158 CATATG CA^TATG
4912 CATATG
PflMI 1709 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3001 CCATCCTATGG
PstI 4264 CTGCAG CTGCA^G
4794 CTGCAG
PvuII 2851 CAGCTG CAG^CTG
4858 CAGCTG
Van91I 1709 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3001 CCATCCTATGG
XcmI 1988 CCATTTGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2095 CCACTCAGCTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApaI ApoI AseI AsnI
AspHI AvrII BanII BbvI BclI
BcnI BfaI BlnI BmyI BpmI
BsaBI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRI EcoRII EcoRV
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XhoII