##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI Asp700I Asp718I AspI AviII
BamHI BbeI BcgI BfrI BlpI
Bpu1102I BsiWI BspDI BsrBI BstBI
CelII ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI KasI KpnI KspI MluI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI Psp1406I RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1661 GACGTC GACGT^C
Alw44I 4614 GTGCAC G^TGCAC
AocI 88 CCTTAGG CC^T[ACGT]AGG
ApaLI 4614 GTGCAC G^TGCAC
AseI 3085 ATTAAT AT^TAAT
AsnI 3085 ATTAAT AT^TAAT
BclI 4035 TGATCA T^GATCA
BglI 2699 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3437 AGATCT A^GATCT
BsaBI 4030 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4474 CGATCG CG[GA][CT]^CG
Bsp1407I 2272 TGTACA T^GTACA
BsrGI 2272 TGTACA T^GTACA
BssHII 1158 GCGCGC G^CGCGC
Bst1107I 4172 GTATAC GTA^TAC
BstEII 2122 GGTCACC G^GT[ACGT]ACC
BstPI 2122 GGTCACC G^GT[ACGT]ACC
BstZ17I 4172 GTATAC GTA^TAC
Bsu36I 88 CCTTAGG CC^T[ACGT]AGG
Eco81I 88 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2122 GGTCACC G^GT[ACGT]ACC
FbaI 4035 TGATCA T^GATCA
HindIII 2283 AAGCTT A^AGCTT
MamI 4030 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 2948 CTCGAG C^TCGAG
PmeI 4008 GTTTAAAC GTTT^AAAC
PshAI 1297 GACTTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3085 ATTAAT AT^TAAT
PvuI 4474 CGATCG CGAT^CG
PvuII 2276 CAGCTG CAG^CTG
SexAI 286 ACCTGGT A^CC[AT]GGT
SpeI 1825 ACTAGT A^CTAGT
SphI 1732 GCATGC GCATG^C
SspBI 2272 TGTACA T^GTACA
XhoI 2948 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1983 GTCTAC GT^[AC][GT]AC
4172 GTATAC
AccIII 3503 TCCGGA T^CCGGA
3621 TCCGGA
AcyI 1661 GACGTC G[GA]^CG[CT]C
2696 GACGCC
AvrII 1240 CCTAGG C^CTAGG
4812 CCTAGG
BalI 306 TGGCCA TGG^CCA
3587 TGGCCA
BlnI 1240 CCTAGG C^CTAGG
4812 CCTAGG
BsaHI 1661 GACGTC G[GA]^CG[CT]C
2696 GACGCC
BseAI 3503 TCCGGA T^CCGGA
3621 TCCGGA
BseRI 1110 GAGGAG GAGGAG(10/8)
3213 GAGGAG
BsmI 1756 GCATTC GAATGC(1/-1)
4734 GCATTC
BspEI 3503 TCCGGA T^CCGGA
3621 TCCGGA
BspHI 1106 TCATGA T^CATGA
3094 TCATGA
BssSI 3696 CTCGTG CACGAG(-5/-1)
4314 CTCGTG
BstAPI 320 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2017 GCAGGTGCTGC
DraI 2381 TTTAAA TTT^AAA
4009 TTTAAA
EcoRI 419 GAATTC G^AATTC
1619 GAATTC
HaeII 2004 GGCGCT [GA]GCGC^[CT]
2582 GGCGCT
Hin1I 1661 GACGTC G[GA]^CG[CT]C
2696 GACGCC
HpaI 3559 GTTAAC GTT^AAC
4150 GTTAAC
MfeI 1950 CAATTG C^AATTG
4015 CAATTG
MluNI 306 TGGCCA TGG^CCA
3587 TGGCCA
MroI 3503 TCCGGA T^CCGGA
3621 TCCGGA
MscI 306 TGGCCA TGG^CCA
3587 TGGCCA
MunI 1950 CAATTG C^AATTG
4015 CAATTG
PflMI 925 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1392 CCACCCTTTGG
RcaI 1106 TCATGA T^CATGA
3094 TCATGA
SmaI 180 CCCGGG CCC^GGG
196 CCCGGG
Van91I 925 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1392 CCACCCTTTGG
XmaCI 180 CCCGGG C^CCGGG
196 CCCGGG
XmaI 180 CCCGGG C^CCGGG
196 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI ApaI
ApoI AspEI AspHI AvaI AvaII
BanI BanII BbrPI BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII