##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbrPI
BclI BfrI BlnI BsaAI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BsrDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoRV FbaI FseI
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI TfiI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1688 GACGTC GACGT^C
AlwI 1354 GATCC GGATC(4/5)
AspI 2708 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1251 TGCGCA TGC^GCA
BbeI 1747 GGCGCC GGCGC^C
BbsI 2442 GAAGAC GAAGAC(2/6)
BcgI 881 CGAGCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2740 GTATCC GTATCC(6/5)
BglII 945 AGATCT A^GATCT
BpuAI 2442 GAAGAC GAAGAC(2/6)
BsaBI 2019 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1786 CGACCG CG[GA][CT]^CG
BsmBI 2230 GAGACG CGTCTC(1/5)
BsmI 3668 GCATTC GAATGC(1/-1)
Bsp1407I 1650 TGTACA T^GTACA
BsrBI 492 CCGCTC CCGCTC(-3/-3)
BsrGI 1650 TGTACA T^GTACA
DraI 4136 TTTAAA TTT^AAA
EcoRI 1433 GAATTC G^AATTC
EcoT22I 3633 ATGCAT ATGCA^T
FspI 1251 TGCGCA TGC^GCA
KasI 1747 GGCGCC G^GCGCC
MamI 2019 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1747 GGCGCC GG^CGCC
NsiI 3633 ATGCAT ATGCA^T
PaeR7I 1930 CTCGAG C^TCGAG
PflFI 2708 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 1521 AGTACT AGT^ACT
SexAI 1425 ACCTGGT A^CC[AT]GGT
SfiI 3919 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1747 GGCGCC GGC^GCC
SspBI 1650 TGTACA T^GTACA
Tth111I 2708 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1930 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 702 GTCTAC GT^[AC][GT]AC
1099 GTAGAC
AcsI 1433 GAATTC [GA]^AATT[CT]
2609 GAATTT
AflIII 334 ACATGT A^C[GA][CT]GT
1554 ACATGT
Alw44I 330 GTGCAC G^TGCAC
1697 GTGCAC
Aor51HI 2821 AGCGCT AGC^GCT
3623 AGCGCT
ApaLI 330 GTGCAC G^TGCAC
1697 GTGCAC
ApoI 1433 GAATTC [GA]^AATT[CT]
2609 GAATTT
BspLU11I 334 ACATGT A^CATGT
1554 ACATGT
BsrFI 1228 GCCGGC [GA]^CCGG[CT]
1389 GCCGGC
BssSI 16 CACGAG CACGAG(-5/-1)
1307 CTCGTG
BstEII 271 GGTCACC G^GT[ACGT]ACC
2580 GGTCACC
BstPI 271 GGTCACC G^GT[ACGT]ACC
2580 GGTCACC
BstYI 945 AGATCT [GA]^GATC[CT]
1353 AGATCC
Cfr10I 1228 GCCGGC [GA]^CCGG[CT]
1389 GCCGGC
Eco47III 2821 AGCGCT AGC^GCT
3623 AGCGCT
EcoO65I 271 GGTCACC G^GT[ACGT]ACC
2580 GGTCACC
HgaI 2232 GACGC GACGC(5/10)
1799 GCGTC
HincII 2492 GTTGAC GT[CT]^[GA]AC
4052 GTTGAC
HindII 2492 GTTGAC GT[CT]^[GA]AC
4052 GTTGAC
MflI 945 AGATCT [GA]^GATC[CT]
1353 AGATCC
NaeI 1228 GCCGGC GCC^GGC
1389 GCCGGC
NgoMIV 1228 GCCGGC G^CCGGC
1389 GCCGGC
SbfI 1980 CCTGCAGG CCTGCA^GG
3858 CCTGCAGG
SmaI 2110 CCCGGG CCC^GGG
3346 CCCGGG
Sse8387I 1980 CCTGCAGG CCTGCA^GG
3858 CCTGCAGG
XhoII 945 AGATCT [GA]^GATC[CT]
1353 AGATCC
XmaCI 2110 CCCGGG C^CCGGG
3346 CCCGGG
XmaI 2110 CCCGGG C^CCGGG
3346 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI